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The map label for this gene is proC [H]

Identifier: 227080670

GI number: 227080670

Start: 474458

End: 475276

Strand: Reverse

Name: proC [H]

Synonym: VCM66_0445

Alternate gene names: 227080670

Gene position: 475276-474458 (Counterclockwise)

Preceding gene: 227080671

Following gene: 227080669

Centisome position: 16.43

GC content: 53.85

Gene sequence:

>819_bases
ATGGAACAGAGAAGCATCGCCTTTATTGGCGCAGGTAACATGGCACGCGCCATCATTGCGGGATTGATTGCCAGTGGTTA
TGCCGCGAATAACATCGTTGCAACCGCCCCTTCACTCACGCGCCGCGAGCCTCTTGAAGCGCAATACGGTATTCGTACCA
CCAGCGATAACCTTGCTGCGGCGCAACAAGCGGATGTGGTGGTGTTAGCGGTCAAACCGCAGCTGATGGCTGAGGTGTGT
AAACCGCTGCAAGCGGTCGATTTCTCTGGAAAGTTGGTTATCTCGATTGCGGCGGGTGTGTCAGTCAAAAGACTCAATGA
GATGCTGGCCACCCAGCTCAATTTAGTGCGTGTGATGCCCAATACGCCATCACTGGTAAACCTTGGCATGAGCGGTTTGT
ACGCCCCTGATTCGGTGAGCGAAGTGGATAAAGCCTTTACCACACAACTGATGCAAAGCGTGGGCGAAGTGTGCTGGGTA
GACAACGAATCTGGCATCAACAGCGTGATTGCTGCCGCAGGGAGTGCTCCTGCTTATTTCTTCTTGTTTATGCAAGCTAT
GCAGCAAGAGGCCATGGCGCAAGGCTTTGATGAAGCCACCGCCCGTTTGTTAGTACAGCAATCGGCTCTCGGTGCGGCCG
CCATGGTCAAAGCCAACCCAGAACTCTCACTGACCACTTTGCGTGAACAAGTGACGTCAAAAGGCGGCACCACGGCGCAA
GCGATCCAGACGTTTAACGATCACCAACTCAGCGACATCGTGGCCAAAGCGATGCAAGCGGCGGTAACACGCGCGCAAGA
GATGGAACAACTGTTTTAA

Upstream 100 bases:

>100_bases
ATTGGTACCGCGATTTTCGGCGAGCGCGATTACAGCCGCAACGCTTAACGTTTGGGATGCTCGTTGCAACGAGTGCCCCT
TTTAGATTTGAGAGTATCGA

Downstream 100 bases:

>100_bases
TGTTTAAGGCGTCCACTCAAACTGAGCGGTCGCCAAATCAATAAGGGCAACTTATGAATTCACTGAGCTTTCTCATCAAC
ACCCTATTTGACCTCTACAT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MEQRSIAFIGAGNMARAIIAGLIASGYAANNIVATAPSLTRREPLEAQYGIRTTSDNLAAAQQADVVVLAVKPQLMAEVC
KPLQAVDFSGKLVISIAAGVSVKRLNEMLATQLNLVRVMPNTPSLVNLGMSGLYAPDSVSEVDKAFTTQLMQSVGEVCWV
DNESGINSVIAAAGSAPAYFFLFMQAMQQEAMAQGFDEATARLLVQQSALGAAAMVKANPELSLTTLREQVTSKGGTTAQ
AIQTFNDHQLSDIVAKAMQAAVTRAQEMEQLF

Sequences:

>Translated_272_residues
MEQRSIAFIGAGNMARAIIAGLIASGYAANNIVATAPSLTRREPLEAQYGIRTTSDNLAAAQQADVVVLAVKPQLMAEVC
KPLQAVDFSGKLVISIAAGVSVKRLNEMLATQLNLVRVMPNTPSLVNLGMSGLYAPDSVSEVDKAFTTQLMQSVGEVCWV
DNESGINSVIAAAGSAPAYFFLFMQAMQQEAMAQGFDEATARLLVQQSALGAAAMVKANPELSLTTLREQVTSKGGTTAQ
AIQTFNDHQLSDIVAKAMQAAVTRAQEMEQLF
>Mature_272_residues
MEQRSIAFIGAGNMARAIIAGLIASGYAANNIVATAPSLTRREPLEAQYGIRTTSDNLAAAQQADVVVLAVKPQLMAEVC
KPLQAVDFSGKLVISIAAGVSVKRLNEMLATQLNLVRVMPNTPSLVNLGMSGLYAPDSVSEVDKAFTTQLMQSVGEVCWV
DNESGINSVIAAAGSAPAYFFLFMQAMQQEAMAQGFDEATARLLVQQSALGAAAMVKANPELSLTTLREQVTSKGGTTAQ
AIQTFNDHQLSDIVAKAMQAAVTRAQEMEQLF

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=274, Percent_Identity=31.021897810219, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI24797095, Length=274, Percent_Identity=31.021897810219, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI198041662, Length=274, Percent_Identity=29.9270072992701, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI21361454, Length=274, Percent_Identity=31.021897810219, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=34.2007434944238, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569021, Length=265, Percent_Identity=33.2075471698113, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17540664, Length=287, Percent_Identity=30.3135888501742, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320861, Length=290, Percent_Identity=25.8620689655172, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=32.8301886792453, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=29.2418772563177, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24647700, Length=170, Percent_Identity=31.1764705882353, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 28786; Mature: 28786

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQRSIAFIGAGNMARAIIAGLIASGYAANNIVATAPSLTRREPLEAQYGIRTTSDNLAA
CCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHCCCEECCCCHHH
AQQADVVVLAVKPQLMAEVCKPLQAVDFSGKLVISIAAGVSVKRLNEMLATQLNLVRVMP
HHHCCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEECC
NTPSLVNLGMSGLYAPDSVSEVDKAFTTQLMQSVGEVCWVDNESGINSVIAAAGSAPAYF
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCHHHH
FLFMQAMQQEAMAQGFDEATARLLVQQSALGAAAMVKANPELSLTTLREQVTSKGGTTAQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHCCCCHHH
AIQTFNDHQLSDIVAKAMQAAVTRAQEMEQLF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEQRSIAFIGAGNMARAIIAGLIASGYAANNIVATAPSLTRREPLEAQYGIRTTSDNLAA
CCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHCCCEECCCCHHH
AQQADVVVLAVKPQLMAEVCKPLQAVDFSGKLVISIAAGVSVKRLNEMLATQLNLVRVMP
HHHCCEEEEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEECC
NTPSLVNLGMSGLYAPDSVSEVDKAFTTQLMQSVGEVCWVDNESGINSVIAAAGSAPAYF
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCHHHH
FLFMQAMQQEAMAQGFDEATARLLVQQSALGAAAMVKANPELSLTTLREQVTSKGGTTAQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHCCCCHHH
AIQTFNDHQLSDIVAKAMQAAVTRAQEMEQLF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8982386 [H]