Click here to switch to the map view.

The map label for this gene is ksgA [H]

Identifier: 227080654

GI number: 227080654

Start: 456443

End: 457291

Strand: Reverse

Name: ksgA [H]

Synonym: VCM66_0428

Alternate gene names: 227080654

Gene position: 457291-456443 (Counterclockwise)

Preceding gene: 227080655

Following gene: 227080653

Centisome position: 15.81

GC content: 47.7

Gene sequence:

>849_bases
TTGATAAAAAAGCGCAATAACTTAGAGAAAACTATGAGAAATGATGTCCACTTAGGGCACAAAGCGCGTAAACGCTTTGG
TCAGAACTTCCTCAATGATCCTTACATCATTGATGGTATTGTGTCGGCAATTAATCCCAAACCGGGGCAAAACTTGGTTG
AAATCGGCCCAGGGTTAGGCGCCATCACAGAACCGGTAGGACGTGAAGTGGATAAGTTCACTGTCATTGAGCTTGACCGC
GATCTGGCAGAACGCCTGCGTAATCATCCAGAATTAGCCAGCAAGTTGACCATTCATGAAGGCGATGCGATGCGTTTCGA
CTTCAAGCAGTTGGTGAAACCCAATAACAAACTTCGCGTGTTTGGTAACTTACCTTACAACATTTCAACGCCGCTGATGT
TCCACTTGTTTGAATTCCACCGTGATATTCAAGACATGCACTTCATGCTGCAAAAAGAGGTGGTGAACCGATTAGCCGCA
GGTCCGGGAACCAAGGCCTACGGTCGACTAACCGTGATGGCGCAATACTACTGTAAAGTGGTTCCGGTACTAGAAGTGCC
ACCTAGCGCATTTGTGCCACCACCAAAAGTGGATTCGGCCGTGGTACGTTTGGTGCCTTATGAAGATCTCCCTCATCCAG
CAACCAGCTTAGAGTGGTTAGATCGCGTGGTACGTGAAGGCTTTAACCAACGCCGTAAAACGGTGCGTAACTGTTATAAA
GGTTTGGCTGAACCGGAAACACTGGAAACTCTGGGCATCAACCCAGGTATGCGCCCAGAAAATCTGACCTTGGCTCAATT
TGTGGCATTAGCGAACTGGCTTGATGCCACACACAAAACTCACGCATAA

Upstream 100 bases:

>100_bases
CGCACTTCGGTCGATCACGGTACGGCACTCGATCTGGCTGGAACGGGTCAAGCCGATGCGGGAAGCTTTTGGACTGCATT
AGCTTACGCAATTGAATTAG

Downstream 100 bases:

>100_bases
TCGATAAGGACGACATTCAGTCGTCCTTTGTTTATCTGCCAATATCTGACTACTAAGCTGAGGAGTCGCTCATGGATGTT
TCGCTGCCCTGTATCAAAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MIKKRNNLEKTMRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDR
DLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAA
GPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYK
GLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA

Sequences:

>Translated_282_residues
MIKKRNNLEKTMRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDR
DLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAA
GPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYK
GLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
>Mature_282_residues
MIKKRNNLEKTMRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDR
DLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAA
GPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYK
GLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=293, Percent_Identity=32.4232081911263, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI7657198, Length=251, Percent_Identity=32.6693227091633, Blast_Score=114, Evalue=7e-26,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=66.2878787878788, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=35.1598173515982, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=29.4915254237288, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=233, Percent_Identity=30.4721030042918, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=34.375, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21358017, Length=241, Percent_Identity=34.0248962655602, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32046; Mature: 32046

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRNNLEKTMRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCCCCC
AITEPVGREVDKFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRV
HHHHHHCCCCCCEEEEECCHHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHCCCCCCEEE
FGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYK
HHHEECCCCCCCCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
CCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKKRNNLEKTMRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCCCCC
AITEPVGREVDKFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRV
HHHHHHCCCCCCEEEEECCHHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHCCCCCCEEE
FGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYK
HHHEECCCCCCCCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
CCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA