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The map label for this gene is dusB [H]

Identifier: 227080505

GI number: 227080505

Start: 284935

End: 285930

Strand: Reverse

Name: dusB [H]

Synonym: VCM66_0276

Alternate gene names: 227080505

Gene position: 285930-284935 (Counterclockwise)

Preceding gene: 227080506

Following gene: 227080504

Centisome position: 9.89

GC content: 50.0

Gene sequence:

>996_bases
TTGCTGGTACGAAGCTGTGAAGACGTTTTGAAAATCGGAAATCATCAACTTAAGAACAATCTGATCGTCGCCCCGATGGC
GGGTGTCACCGACAGACCCTTTCGAGAGTTGTGTTTACGCTACGGGGCAGGAATGGCGGTCAGTGAAATGATGTCATGCA
ATCCTGCGTTGTGGAAAACGGCCAAATCGCAAAACCGCATGGTACATGAAGGCGAATCGGGCATTCGCTCAGTACAAATT
GCGGGCAGCGATCCACAGCTGATGGCCGAAGCAGCGCAGTTCAGCGTTGAAAACGGTGCGCAAATCATCGATATCAACAT
GGGCTGCCCAGCCAAAAAAGTAAATAAAAAGCTCGCGGGCTCTGCACTGCTGCGCTATCCGGATGTGATTGAAGACATCT
TGAAAGCGGTAGTGAATGCAGTGAATGTGCCAGTCACACTCAAAACCCGAACGGGTTGGGATACAGACAATAAAAACTGT
CTCTCGATTGCTCAATTAGCCGAAGACTGCGGCATACAAGCACTGGCTCTCCATGGACGAACTCGTGCCTGCATGTACAA
AGGTGAGGCGGAATACGACAGCATTAAAGCGGTCAAAGCGGCTGTGAGCATTCCGGTTATCGCTAACGGTGATATCGATA
GCCCGGAGAAAGCACGCTACGTACTGGAGTACACCGGTGCAGACGCTTTAATGATTGGCCGTCCTGCCCAAGGACGACCA
TGGATTTTTCAGGAAATCCAACACTTTTTGGAACACGGCACCACAATGCCAGAACTTCCAATTTCGGAAGTGAAAGACAT
CATGCTTGGTCATGTAACCGCACTCCATCAGTTTTATGGAGAGTACTTAGGCCCACGAATTGCGCGCAAACACGTGAGTT
GGTACCTGCAAGAGCATGAACAAGCGAGTGCGTTTCGCCGTACCTTCAACGCCATTGAGACTGCTGACCAGCAACTCGAT
GCGCTCGAAGGGTATTTTGATAACGTTGCATCATAA

Upstream 100 bases:

>100_bases
GGCCTAATTGACTGATAAATCGACTATTTGGCAAAACAATTTGCCAATGCTCAAATATTAGTCTTTTCAGGCATCAAAAA
AATGCGTAAAATGCGCGCCC

Downstream 100 bases:

>100_bases
TTAAGAGAAGAGCTAGACCGAATATGTTCGAACAAAATCTGACTTCAGAAGCTTTAACCGTTACTACCGTAACTTCACAA
GACCAAATCACGCAAAAGCC

Product: NifR3/Smm1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MLVRSCEDVLKIGNHQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSCNPALWKTAKSQNRMVHEGESGIRSVQI
AGSDPQLMAEAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLRYPDVIEDILKAVVNAVNVPVTLKTRTGWDTDNKNC
LSIAQLAEDCGIQALALHGRTRACMYKGEAEYDSIKAVKAAVSIPVIANGDIDSPEKARYVLEYTGADALMIGRPAQGRP
WIFQEIQHFLEHGTTMPELPISEVKDIMLGHVTALHQFYGEYLGPRIARKHVSWYLQEHEQASAFRRTFNAIETADQQLD
ALEGYFDNVAS

Sequences:

>Translated_331_residues
MLVRSCEDVLKIGNHQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSCNPALWKTAKSQNRMVHEGESGIRSVQI
AGSDPQLMAEAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLRYPDVIEDILKAVVNAVNVPVTLKTRTGWDTDNKNC
LSIAQLAEDCGIQALALHGRTRACMYKGEAEYDSIKAVKAAVSIPVIANGDIDSPEKARYVLEYTGADALMIGRPAQGRP
WIFQEIQHFLEHGTTMPELPISEVKDIMLGHVTALHQFYGEYLGPRIARKHVSWYLQEHEQASAFRRTFNAIETADQQLD
ALEGYFDNVAS
>Mature_331_residues
MLVRSCEDVLKIGNHQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSCNPALWKTAKSQNRMVHEGESGIRSVQI
AGSDPQLMAEAAQFSVENGAQIIDINMGCPAKKVNKKLAGSALLRYPDVIEDILKAVVNAVNVPVTLKTRTGWDTDNKNC
LSIAQLAEDCGIQALALHGRTRACMYKGEAEYDSIKAVKAAVSIPVIANGDIDSPEKARYVLEYTGADALMIGRPAQGRP
WIFQEIQHFLEHGTTMPELPISEVKDIMLGHVTALHQFYGEYLGPRIARKHVSWYLQEHEQASAFRRTFNAIETADQQLD
ALEGYFDNVAS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=31.6239316239316, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI239788462, Length=260, Percent_Identity=31.1538461538462, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI31742496, Length=310, Percent_Identity=29.0322580645161, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=34, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI40807366, Length=277, Percent_Identity=28.158844765343, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=69.7819314641744, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1788462, Length=297, Percent_Identity=30.976430976431, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693211, Length=308, Percent_Identity=25, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=251, Percent_Identity=31.8725099601594, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=266, Percent_Identity=32.3308270676692, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=31.3636363636364, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=246, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=31.8777292576419, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=322, Percent_Identity=25.776397515528, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=32.7731092436975, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=29.5154185022026, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=29.5154185022026, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=31.0204081632653, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921524, Length=310, Percent_Identity=24.1935483870968, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36488; Mature: 36488

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVRSCEDVLKIGNHQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSCNPALWKT
CCCCCHHHHHHHCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AKSQNRMVHEGESGIRSVQIAGSDPQLMAEAAQFSVENGAQIIDINMGCPAKKVNKKLAG
HHHCCCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
SALLRYPDVIEDILKAVVNAVNVPVTLKTRTGWDTDNKNCLSIAQLAEDCGIQALALHGR
HHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
TRACMYKGEAEYDSIKAVKAAVSIPVIANGDIDSPEKARYVLEYTGADALMIGRPAQGRP
CCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHEEEEEECCCCEEEECCCCCCCC
WIFQEIQHFLEHGTTMPELPISEVKDIMLGHVTALHQFYGEYLGPRIARKHVSWYLQEHE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QASAFRRTFNAIETADQQLDALEGYFDNVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVRSCEDVLKIGNHQLKNNLIVAPMAGVTDRPFRELCLRYGAGMAVSEMMSCNPALWKT
CCCCCHHHHHHHCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHH
AKSQNRMVHEGESGIRSVQIAGSDPQLMAEAAQFSVENGAQIIDINMGCPAKKVNKKLAG
HHHCCCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
SALLRYPDVIEDILKAVVNAVNVPVTLKTRTGWDTDNKNCLSIAQLAEDCGIQALALHGR
HHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
TRACMYKGEAEYDSIKAVKAAVSIPVIANGDIDSPEKARYVLEYTGADALMIGRPAQGRP
CCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHEEEEEECCCCEEEECCCCCCCC
WIFQEIQHFLEHGTTMPELPISEVKDIMLGHVTALHQFYGEYLGPRIARKHVSWYLQEHE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QASAFRRTFNAIETADQQLDALEGYFDNVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]