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The map label for this gene is gntR [H]

Identifier: 227080503

GI number: 227080503

Start: 283479

End: 284477

Strand: Reverse

Name: gntR [H]

Synonym: VCM66_0274

Alternate gene names: 227080503

Gene position: 284477-283479 (Counterclockwise)

Preceding gene: 227080504

Following gene: 227080500

Centisome position: 9.83

GC content: 51.55

Gene sequence:

>999_bases
ATGACAAAAAAACAGCGCGCGACATTGCAAGATGTGGCGGATCAAGTTGGCGTAACAAAAATGACTGTGTCGCGTTATCT
GAGAAGCCCTGATTCCGTGGCTGCGGCCACGCGGGAGAAGATAGCGCTCGCCGTTGAGGCTTTGGGATACATTGAAAATC
GAGCCCCTGCGATGCTTTCTAAATCATCCAGTAAGGCGATTGGTATTTTGCTTCCTTCCTTGTCGAACCAAATTTTTGCC
TCTTTTGTGCAAGGGATTGAAGCGGTGACCAAAGCCAATGGCTATGAAACCCTACTCGCCCACTTTGGTTACGATGAGGA
GGAAGAAGAGCGCAAAATTGCTTCGCTGCTCGCGTATCAAGTCGATGGTCTTATTCTCACCGAAAGTCATCACACTCAGC
GCACTTTGCAGATGATTGCCAGCTCTGGCGTGCCGGTAGTAGAAACCATGGAGCTTCCCGCCAACCCAATTGACATGGCA
GTAGGCATGGATCACGTTGAAGCCTCATATCAAGCGGTGAAAAAAATTATCGCGGCGGGTAAACGTTCGATTGCCTATTT
CGGCGCACGGCTTGATACCCGCACCAAACTGCGTATGCAGGGCTATGACCAAGCGATGCAAGAAGCCGGGCTGCCTATCA
AGCACGTGCTGACTGGCTCACACTCCAGTTTCTCTCTCGCCGCTCAGTTGCTTGATGAAGCCTTCGCGCGTTACCCCGAT
CTGGATGGTGTGTTCTGCACCAACGATGACATCGCGATTGGTACTCTACTTGTGGCGCAGCAGCGTGGGATTCGGGTACC
CGAACAGCTTTCCGTGATTGGCTATAACGCGCTGGACATCGGCCGCACCATCACGCCAAAGCTGACTAGCGTTGATTCGC
CACGTTATGCGATTGGTGAAAAAAGCGCTGAGCTACTGATTGCCGCACTCAAAGGTGAGCGCGCTGAAGAACATATAGTG
GATATGGGTTATCGTTTTACTGCGGGTGAAAGTGTGTAA

Upstream 100 bases:

>100_bases
CTTTTTTATTACCTTTTATTCACCACACACAATGGTTGTACCCATACCGTCACTTCTTCATAATGTTACTCATAACATTT
TATAAAAGACTCAATAACCT

Downstream 100 bases:

>100_bases
CACCAGCACTCCACAAATGATAAAGATGAAAAAGGCGGTATACACCGCCTTTTCTATGTTGTACATCCAGCAAGGTTAAA
CAATAAAGCTCGCCCCTTGT

Product: gluconate utilization system gnt-I transcriptional repressor

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTKKQRATLQDVADQVGVTKMTVSRYLRSPDSVAAATREKIALAVEALGYIENRAPAMLSKSSSKAIGILLPSLSNQIFA
SFVQGIEAVTKANGYETLLAHFGYDEEEEERKIASLLAYQVDGLILTESHHTQRTLQMIASSGVPVVETMELPANPIDMA
VGMDHVEASYQAVKKIIAAGKRSIAYFGARLDTRTKLRMQGYDQAMQEAGLPIKHVLTGSHSSFSLAAQLLDEAFARYPD
LDGVFCTNDDIAIGTLLVAQQRGIRVPEQLSVIGYNALDIGRTITPKLTSVDSPRYAIGEKSAELLIAALKGERAEEHIV
DMGYRFTAGESV

Sequences:

>Translated_332_residues
MTKKQRATLQDVADQVGVTKMTVSRYLRSPDSVAAATREKIALAVEALGYIENRAPAMLSKSSSKAIGILLPSLSNQIFA
SFVQGIEAVTKANGYETLLAHFGYDEEEEERKIASLLAYQVDGLILTESHHTQRTLQMIASSGVPVVETMELPANPIDMA
VGMDHVEASYQAVKKIIAAGKRSIAYFGARLDTRTKLRMQGYDQAMQEAGLPIKHVLTGSHSSFSLAAQLLDEAFARYPD
LDGVFCTNDDIAIGTLLVAQQRGIRVPEQLSVIGYNALDIGRTITPKLTSVDSPRYAIGEKSAELLIAALKGERAEEHIV
DMGYRFTAGESV
>Mature_331_residues
TKKQRATLQDVADQVGVTKMTVSRYLRSPDSVAAATREKIALAVEALGYIENRAPAMLSKSSSKAIGILLPSLSNQIFAS
FVQGIEAVTKANGYETLLAHFGYDEEEEERKIASLLAYQVDGLILTESHHTQRTLQMIASSGVPVVETMELPANPIDMAV
GMDHVEASYQAVKKIIAAGKRSIAYFGARLDTRTKLRMQGYDQAMQEAGLPIKHVLTGSHSSFSLAAQLLDEAFARYPDL
DGVFCTNDDIAIGTLLVAQQRGIRVPEQLSVIGYNALDIGRTITPKLTSVDSPRYAIGEKSAELLIAALKGERAEEHIVD
MGYRFTAGESV

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=50.3030303030303, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=43.8066465256798, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1790369, Length=325, Percent_Identity=28.6153846153846, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790194, Length=316, Percent_Identity=29.746835443038, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=29.9319727891156, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=24.8275862068966, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=25.7328990228013, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=24.6153846153846, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787906, Length=262, Percent_Identity=27.8625954198473, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=25.9615384615385, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=23.8853503184713, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36050; Mature: 35918

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKQRATLQDVADQVGVTKMTVSRYLRSPDSVAAATREKIALAVEALGYIENRAPAMLS
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
KSSSKAIGILLPSLSNQIFASFVQGIEAVTKANGYETLLAHFGYDEEEEERKIASLLAYQ
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
VDGLILTESHHTQRTLQMIASSGVPVVETMELPANPIDMAVGMDHVEASYQAVKKIIAAG
HCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHCC
KRSIAYFGARLDTRTKLRMQGYDQAMQEAGLPIKHVLTGSHSSFSLAAQLLDEAFARYPD
CHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHHHCCC
LDGVFCTNDDIAIGTLLVAQQRGIRVPEQLSVIGYNALDIGRTITPKLTSVDSPRYAIGE
CCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHCC
KSAELLIAALKGERAEEHIVDMGYRFTAGESV
CHHHHHHHHHCCCCHHHHHHHCCCEECCCCCC
>Mature Secondary Structure 
TKKQRATLQDVADQVGVTKMTVSRYLRSPDSVAAATREKIALAVEALGYIENRAPAMLS
CCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
KSSSKAIGILLPSLSNQIFASFVQGIEAVTKANGYETLLAHFGYDEEEEERKIASLLAYQ
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHH
VDGLILTESHHTQRTLQMIASSGVPVVETMELPANPIDMAVGMDHVEASYQAVKKIIAAG
HCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHCC
KRSIAYFGARLDTRTKLRMQGYDQAMQEAGLPIKHVLTGSHSSFSLAAQLLDEAFARYPD
CHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHHHCCC
LDGVFCTNDDIAIGTLLVAQQRGIRVPEQLSVIGYNALDIGRTITPKLTSVDSPRYAIGE
CCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHCC
KSAELLIAALKGERAEEHIVDMGYRFTAGESV
CHHHHHHHHHCCCCHHHHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]