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The map label for this gene is dppA [H]

Identifier: 227080404

GI number: 227080404

Start: 170519

End: 172147

Strand: Direct

Name: dppA [H]

Synonym: VCM66_0170

Alternate gene names: 227080404

Gene position: 170519-172147 (Clockwise)

Preceding gene: 227080403

Following gene: 227080405

Centisome position: 5.9

GC content: 47.7

Gene sequence:

>1629_bases
TTGGGTTGGCGATTGTTACAAAAGCAAATACAACAAACTAGGGATCTGGATTCCCGCATAAGGAGTTATGCAATGAAAAC
CATGAAAAACAAACTGGCGTTGGCTTTGATAGCGGCTGGCTTGAGTTTTGGTGCGATGGCAGCGGATATTACCGTGGCTT
ACGACGCTGACCCGGTCTCAATGGACCCACATGAGCAGCTGTCAGGCGGTACTCTGCAACTGTCGCACATGGTCTTTGAT
CCACTTATTCGCTTTACACAAAAAATGGAATTTGAACCTCGTCTGGCCGAATCTTGGCAGCGTGTGGATAACGAAACCAT
GCGTTTCACTTTGCGCAAAGGCGTGAAGTTCCACTCTGGCAATGATTTTACTGCGGATGACGTGGTATGGACGTTCAATC
GTTTGAAAGAATCGGCGGATTTTAAAGGGATTTTTGAACCTCTGGTCTCTTTGACTAAGGTGGATGATTACACCATCGAA
ATCAAAACGGCTGGCACTTACCCGCTGATTGAAAACGTGGCGACCTACATTTTCCCGATGGACAGCAAGTTCTACACCGG
CACCACGGCGGATGGTAAAAACAAAGCAGAAATCGTTAAGCACGGTAACTCTTTTGCCTCTGAAAATATCTCTGGTACCG
GGCCATTTACGGTGACGGCTCGTGAGCAAGGCGTGAAACTTGAGTTCCAACGCAACCCCAATTACTGGGATAAAGCGTCG
AAAGGCAATGTGGACAAACTGACGCTGGCAGTGATTAAAGAAGATGCAACTCGCGTTGCGGCACTGCTGTCTGGCGGTGT
GGATATGATTGCACCGGTTTCTCCGAATGATTACCAACGCATCAAAGATGCGAAAGGTGTAGATCTCTTCACTATGCCGG
GCACTCGTGTGATTACCTTCCAAATGAACCAAAACAGTAACCCAGCGTTGAAAGATGTGCGTGTGCGTCAAGCGATTGTC
TACGCCATCAACAATGAAGGGATTGTCGATAAGGTCATGAAAGGGGCAGCTACCACGGCTGCGCAGCAAAGCCCAGTCGG
TTATGCCGGTTACAATGAGAACCTAAAGCCACGTTTTGATCTCAACAAAGCGAAAGAGCTGATGAAAGAAGCAGGTTATG
AGAAAGGCTTCAACCTGACCATGATCGCGCCAAACAACCGTTACGTGAACGATGAGAAGATCGCGCAAGCGGCATCGGCG
ATGTTGTCAAAAATCGGGATTAAAGTTGACCTAAAAACCATGCCAAAAGCGCAATACTGGCCTGAGTTTGATAAGTGTGC
GGCAGATATGCTGATGATTGGTTGGCACCCAGATACAGAAGACTCAGCAAACTTTACTGAGTTCCTGACCATGACCCGTG
ATTCCAATACGGGTAAAGGTCAGTACAACTGTGGTTACTACTCGAATGCGGAAGTCGATAAGTTAGTCAATGCCGCGAAC
GAGGAAACCGATCTAGAAAAACGCAGCACCATGCTGAAGAAAGTGGAAGAGATCCTGTATAACGAAGCGGCGTTTGTGCC
TCTTCACTTCCAAGATCCGTCATGGGCAGCGAAAAATACGCTAGATATCGCACCTATCATCAACGGTATGGACTTCCCAT
ACTTTGGCGATCTGGTGGTGAAAGAGTAA

Upstream 100 bases:

>100_bases
CCGCAGCATGAATACAGCAAGAAATTGATTTCCTTAATGCCTGAATTTACAGGCTTACGCGAAAGCACTACGGCAGCATA
GCCGTTAACCATGGACCAAC

Downstream 100 bases:

>100_bases
TCTCGGACCACTCTGCTCTGTAGGCGTTGGTCTACAGAGCTTCTCTTCGGGTACAGGGTTTTGTTTCAGTCGGGCCCCGT
ACCCTTTTTCAGACTGAAAG

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MGWRLLQKQIQQTRDLDSRIRSYAMKTMKNKLALALIAAGLSFGAMAADITVAYDADPVSMDPHEQLSGGTLQLSHMVFD
PLIRFTQKMEFEPRLAESWQRVDNETMRFTLRKGVKFHSGNDFTADDVVWTFNRLKESADFKGIFEPLVSLTKVDDYTIE
IKTAGTYPLIENVATYIFPMDSKFYTGTTADGKNKAEIVKHGNSFASENISGTGPFTVTAREQGVKLEFQRNPNYWDKAS
KGNVDKLTLAVIKEDATRVAALLSGGVDMIAPVSPNDYQRIKDAKGVDLFTMPGTRVITFQMNQNSNPALKDVRVRQAIV
YAINNEGIVDKVMKGAATTAAQQSPVGYAGYNENLKPRFDLNKAKELMKEAGYEKGFNLTMIAPNNRYVNDEKIAQAASA
MLSKIGIKVDLKTMPKAQYWPEFDKCAADMLMIGWHPDTEDSANFTEFLTMTRDSNTGKGQYNCGYYSNAEVDKLVNAAN
EETDLEKRSTMLKKVEEILYNEAAFVPLHFQDPSWAAKNTLDIAPIINGMDFPYFGDLVVKE

Sequences:

>Translated_542_residues
MGWRLLQKQIQQTRDLDSRIRSYAMKTMKNKLALALIAAGLSFGAMAADITVAYDADPVSMDPHEQLSGGTLQLSHMVFD
PLIRFTQKMEFEPRLAESWQRVDNETMRFTLRKGVKFHSGNDFTADDVVWTFNRLKESADFKGIFEPLVSLTKVDDYTIE
IKTAGTYPLIENVATYIFPMDSKFYTGTTADGKNKAEIVKHGNSFASENISGTGPFTVTAREQGVKLEFQRNPNYWDKAS
KGNVDKLTLAVIKEDATRVAALLSGGVDMIAPVSPNDYQRIKDAKGVDLFTMPGTRVITFQMNQNSNPALKDVRVRQAIV
YAINNEGIVDKVMKGAATTAAQQSPVGYAGYNENLKPRFDLNKAKELMKEAGYEKGFNLTMIAPNNRYVNDEKIAQAASA
MLSKIGIKVDLKTMPKAQYWPEFDKCAADMLMIGWHPDTEDSANFTEFLTMTRDSNTGKGQYNCGYYSNAEVDKLVNAAN
EETDLEKRSTMLKKVEEILYNEAAFVPLHFQDPSWAAKNTLDIAPIINGMDFPYFGDLVVKE
>Mature_541_residues
GWRLLQKQIQQTRDLDSRIRSYAMKTMKNKLALALIAAGLSFGAMAADITVAYDADPVSMDPHEQLSGGTLQLSHMVFDP
LIRFTQKMEFEPRLAESWQRVDNETMRFTLRKGVKFHSGNDFTADDVVWTFNRLKESADFKGIFEPLVSLTKVDDYTIEI
KTAGTYPLIENVATYIFPMDSKFYTGTTADGKNKAEIVKHGNSFASENISGTGPFTVTAREQGVKLEFQRNPNYWDKASK
GNVDKLTLAVIKEDATRVAALLSGGVDMIAPVSPNDYQRIKDAKGVDLFTMPGTRVITFQMNQNSNPALKDVRVRQAIVY
AINNEGIVDKVMKGAATTAAQQSPVGYAGYNENLKPRFDLNKAKELMKEAGYEKGFNLTMIAPNNRYVNDEKIAQAASAM
LSKIGIKVDLKTMPKAQYWPEFDKCAADMLMIGWHPDTEDSANFTEFLTMTRDSNTGKGQYNCGYYSNAEVDKLVNAANE
ETDLEKRSTMLKKVEEILYNEAAFVPLHFQDPSWAAKNTLDIAPIINGMDFPYFGDLVVKE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=505, Percent_Identity=29.5049504950495, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=24.3852459016393, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787762, Length=495, Percent_Identity=26.0606060606061, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87081878, Length=542, Percent_Identity=25.4612546125461, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=24.6628131021195, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=24.7191011235955, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787551, Length=447, Percent_Identity=23.7136465324385, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789887, Length=523, Percent_Identity=23.9005736137667, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60616; Mature: 60484

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWRLLQKQIQQTRDLDSRIRSYAMKTMKNKLALALIAAGLSFGAMAADITVAYDADPVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
MDPHEQLSGGTLQLSHMVFDPLIRFTQKMEFEPRLAESWQRVDNETMRFTLRKGVKFHSG
CCCHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEHHHHCCEEECC
NDFTADDVVWTFNRLKESADFKGIFEPLVSLTKVDDYTIEIKTAGTYPLIENVATYIFPM
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHEEEC
DSKFYTGTTADGKNKAEIVKHGNSFASENISGTGPFTVTAREQGVKLEFQRNPNYWDKAS
CCCEEECCCCCCCCHHHHHHCCCCHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCC
KGNVDKLTLAVIKEDATRVAALLSGGVDMIAPVSPNDYQRIKDAKGVDLFTMPGTRVITF
CCCCCEEEEEEEHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCEEEECCCCEEEEE
QMNQNSNPALKDVRVRQAIVYAINNEGIVDKVMKGAATTAAQQSPVGYAGYNENLKPRFD
EECCCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LNKAKELMKEAGYEKGFNLTMIAPNNRYVNDEKIAQAASAMLSKIGIKVDLKTMPKAQYW
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PEFDKCAADMLMIGWHPDTEDSANFTEFLTMTRDSNTGKGQYNCGYYSNAEVDKLVNAAN
CCHHHHHHCEEEEECCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCC
EETDLEKRSTMLKKVEEILYNEAAFVPLHFQDPSWAAKNTLDIAPIINGMDFPYFGDLVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCCCCCCEEE
KE
CC
>Mature Secondary Structure 
GWRLLQKQIQQTRDLDSRIRSYAMKTMKNKLALALIAAGLSFGAMAADITVAYDADPVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
MDPHEQLSGGTLQLSHMVFDPLIRFTQKMEFEPRLAESWQRVDNETMRFTLRKGVKFHSG
CCCHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEHHHHCCEEECC
NDFTADDVVWTFNRLKESADFKGIFEPLVSLTKVDDYTIEIKTAGTYPLIENVATYIFPM
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHEEEC
DSKFYTGTTADGKNKAEIVKHGNSFASENISGTGPFTVTAREQGVKLEFQRNPNYWDKAS
CCCEEECCCCCCCCHHHHHHCCCCHHCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCC
KGNVDKLTLAVIKEDATRVAALLSGGVDMIAPVSPNDYQRIKDAKGVDLFTMPGTRVITF
CCCCCEEEEEEEHHHHHHHHHHHHCCCCEEECCCCCHHHHHHCCCCCEEEECCCCEEEEE
QMNQNSNPALKDVRVRQAIVYAINNEGIVDKVMKGAATTAAQQSPVGYAGYNENLKPRFD
EECCCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LNKAKELMKEAGYEKGFNLTMIAPNNRYVNDEKIAQAASAMLSKIGIKVDLKTMPKAQYW
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PEFDKCAADMLMIGWHPDTEDSANFTEFLTMTRDSNTGKGQYNCGYYSNAEVDKLVNAAN
CCHHHHHHCEEEEECCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCC
EETDLEKRSTMLKKVEEILYNEAAFVPLHFQDPSWAAKNTLDIAPIINGMDFPYFGDLVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCCCCCCEEE
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]