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The map label for this gene is yfgF [C]

Identifier: 227080366

GI number: 227080366

Start: 123737

End: 126226

Strand: Direct

Name: yfgF [C]

Synonym: VCM66_0130

Alternate gene names: 227080366

Gene position: 123737-126226 (Clockwise)

Preceding gene: 227080365

Following gene: 227080369

Centisome position: 4.28

GC content: 52.09

Gene sequence:

>2490_bases
ATGTTTACGGTCTCGCGTCTCATCCCAGATTTAGCATCGGCCAATCAGGTGCTCGAAACGATGGATCTTCCTCATGGGCA
ATCCATCTTAGTGCAAATTTTCTCTCCTTTGAGTCGCGAGCATGTCGTGCAGCTTGCGCGGCTCATCCGTAGTCGCCATC
CACAAGCTTGCCTGCTTGGATGCAGTACCGAAGAGGTGATTTTCCAAGGGGAAGTGCACCATCAGGTGACGTTGCTGCAA
ATCACCGTGTTTGAGCAAACTTATCTTAGCCGTGCTGTTGTGGATTACAGTGACGATGAGGCTGCCGACGCGGAGCGTTT
AGCTCGCCAGCTCGAGCTCACTAGCATGTCTCGAGCAGTGGTCTGTTTTTCTTGGCAGATGGATACCTTGCAAGTCGCAC
GATTTGCCTTACGCGACACGCAGGGTGCGCCAGTGCCCGTCGCTGGTGGAGCCGCCAAACAAACTCCCAGTGGTCGCTGG
GTTTTGCTCGATGAGGCTTGTTATCAAAACGCCAGTGTGGCTATTGCGCTGCATGGCGAAGCGCTTTATGTCGAGACGGG
TGGTTACACTGAGTGGCAGCCTGTTGGGCGCACCTATCGGGTCACAGCCGTGGAGGGAGATCGGGTCTTACGTTTGGATG
ATGAGCCGATAGAGGCGATTTATCAGCGCAATTTGGGCGCGCAAGCCGACTTACCCCATGACTGGCTGATCAGTTTTCCA
CTGATGAAAGGGGAATGCCGCCACCAAGATCTTTATCTGCCGCTCGGGCTGGCGGAGGAAGGCGGACTGAGGTTTAACCG
CCCACTGGCGCTGCAAGATGAAGTGCGCTTTTGTTTTGATCACCCGAGTTTAACCCTAGAGCGGGTCTATCTCACCGCGC
AGCAACTACAAGCCAAGCAGTGCCAACAAGTGTGGGTGTTTAACTGTGCGCTGCGTTTAAACTTCATGCATGAGAATCAC
GAGTTACAACCGCTTCAGGCGGTGGCGCCGACCGATGGTTGTTACTGTTGGGGAGAGCTGCTGTATGAGCATGGTCAGCA
GCAAGTGATGCATCATAGCATGACGTTTCTTGCCTTGCGTGAAGGGGCGGTGCGTGACGATCTTGTCCCAATCCCCTTGC
CGAGTTATCCGGAAGGGATGACGTCCCCTCTGTTTAATTTGATCCGCCATGCGTTTCATGATTTGGATGCCATGACGGAC
AATTTGGCACAGCAGATCCGCGCTCAAACCTCATTACTGACGGCGAGTTACCGGCGTGACCGACGTACCGGCTTACCCAA
TCGAGTGGTGTTACGTGAGCGATTGGCGAACTTCGCGGCCAATGAGCACCTAATCGCCCTGAAAGTGACCAACTTCAATC
AGATCAATGAGAAGTACGGTTATCCGGTAGGCGATAAGCTGCTTCGTGATTTGAGTGAACAGTTTCAGGTCTTTTTGGAT
CAGAAATTGGCAGGGCAGAGCGGTTTGTATGCGATTGGGGTCGGTGAGTGGGCAACGGTTTTTCGTGCCAAGCTGGACGG
GAAAAGTATTCATAGCCATTTCTATCAGTTTGTGGAGCAGTTGGAGCACGTCAACTTCGAGCCCTACGGTTTACCGAATG
TGGATTATCTGTCGATCTCGTTGTGCGCTGGGTTAGTCAGTCAGGGCGATTTTGCAGAGCATTCGCCGGATGAGCTATTG
CTGCGTGCTATCGAAGCACGCCGCTACGCGTTTAACAACAACCACCATTTCTGTAATGCAGCGCGTTTAAAAGTGCAAGA
AAGTGTTCGTCAGGAGCGGCTGAATTGGCTATCGCGGGTTAGCCGCGCGGTGGTACGTGATGATGTGGTGGTTTATGCGC
AGCCGATTTGTCAGGCGCGTAGTCACATTGTGGCTTCCTACGAATGTTTAGTGCGGATTGAAGATGAAGGGGAAATTATC
TTGCCTGGGAATTTTCTCCCGATCATCACCGATACTCACCTCTATACTCGTTTGAGCCGGCAGATGATAACGCATACCTT
CAATATGATGCGTCATCGCCCGGAGGCGTTTTCGATCAATCTTTCACCGCAAGATTTGATGAGTGAGCGCACCTTGCAGC
ATTTGGAAGCGGCAATCAAATCGGTGGCCGATCCCGCCCGTGTCGGGCTCGAAGTGTTGGAGAGCGAACAAATCAAAGAT
TACGGGCGGATGATTGAAGTGTGTAACCATTTCCGCACACTGGGCGCGACCATCATTGTCGATGATTTTGGCAGTGGTTA
TTCCAATATTGATGAGATAGTGAAGCTTGAGCCGCAGGTGATTAAGCTCGATGGCAGCTTGATCCGCAATATTGATCAGG
ATGTGAAGCAGCGCCGGATTGCCGAGCAGCTCGTGAAATTGTGTCAGGTACTGAATGCGAAAACCGTGGCGGAGTTTGTC
CATAACCAGACGGTTTGTCGCATCAGTGAAGATATGGGTGTGGATTATTTACAAGGCTATTTCTTGGGTCGACCTTCACG
CTTGGGTTAA

Upstream 100 bases:

>100_bases
ATCGAGTCACAAAAATCACATTGCATGGTTATTGAGTATAGTCTGCGGTAACGATTGCATTGCATAATGCCTAAACATTT
CACTGGCTTATCAGCTCTTT

Downstream 100 bases:

>100_bases
CGCAAGATAGACGCGTCATCCCATCTTGATATTTACCGTTCAATCCAACGATGACCGCTTTTGTCGGCTTTAAAAGCGTT
CATCGAATAGTGATAAAACT

Product: GGDEF/EAL family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MFTVSRLIPDLASANQVLETMDLPHGQSILVQIFSPLSREHVVQLARLIRSRHPQACLLGCSTEEVIFQGEVHHQVTLLQ
ITVFEQTYLSRAVVDYSDDEAADAERLARQLELTSMSRAVVCFSWQMDTLQVARFALRDTQGAPVPVAGGAAKQTPSGRW
VLLDEACYQNASVAIALHGEALYVETGGYTEWQPVGRTYRVTAVEGDRVLRLDDEPIEAIYQRNLGAQADLPHDWLISFP
LMKGECRHQDLYLPLGLAEEGGLRFNRPLALQDEVRFCFDHPSLTLERVYLTAQQLQAKQCQQVWVFNCALRLNFMHENH
ELQPLQAVAPTDGCYCWGELLYEHGQQQVMHHSMTFLALREGAVRDDLVPIPLPSYPEGMTSPLFNLIRHAFHDLDAMTD
NLAQQIRAQTSLLTASYRRDRRTGLPNRVVLRERLANFAANEHLIALKVTNFNQINEKYGYPVGDKLLRDLSEQFQVFLD
QKLAGQSGLYAIGVGEWATVFRAKLDGKSIHSHFYQFVEQLEHVNFEPYGLPNVDYLSISLCAGLVSQGDFAEHSPDELL
LRAIEARRYAFNNNHHFCNAARLKVQESVRQERLNWLSRVSRAVVRDDVVVYAQPICQARSHIVASYECLVRIEDEGEII
LPGNFLPIITDTHLYTRLSRQMITHTFNMMRHRPEAFSINLSPQDLMSERTLQHLEAAIKSVADPARVGLEVLESEQIKD
YGRMIEVCNHFRTLGATIIVDDFGSGYSNIDEIVKLEPQVIKLDGSLIRNIDQDVKQRRIAEQLVKLCQVLNAKTVAEFV
HNQTVCRISEDMGVDYLQGYFLGRPSRLG

Sequences:

>Translated_829_residues
MFTVSRLIPDLASANQVLETMDLPHGQSILVQIFSPLSREHVVQLARLIRSRHPQACLLGCSTEEVIFQGEVHHQVTLLQ
ITVFEQTYLSRAVVDYSDDEAADAERLARQLELTSMSRAVVCFSWQMDTLQVARFALRDTQGAPVPVAGGAAKQTPSGRW
VLLDEACYQNASVAIALHGEALYVETGGYTEWQPVGRTYRVTAVEGDRVLRLDDEPIEAIYQRNLGAQADLPHDWLISFP
LMKGECRHQDLYLPLGLAEEGGLRFNRPLALQDEVRFCFDHPSLTLERVYLTAQQLQAKQCQQVWVFNCALRLNFMHENH
ELQPLQAVAPTDGCYCWGELLYEHGQQQVMHHSMTFLALREGAVRDDLVPIPLPSYPEGMTSPLFNLIRHAFHDLDAMTD
NLAQQIRAQTSLLTASYRRDRRTGLPNRVVLRERLANFAANEHLIALKVTNFNQINEKYGYPVGDKLLRDLSEQFQVFLD
QKLAGQSGLYAIGVGEWATVFRAKLDGKSIHSHFYQFVEQLEHVNFEPYGLPNVDYLSISLCAGLVSQGDFAEHSPDELL
LRAIEARRYAFNNNHHFCNAARLKVQESVRQERLNWLSRVSRAVVRDDVVVYAQPICQARSHIVASYECLVRIEDEGEII
LPGNFLPIITDTHLYTRLSRQMITHTFNMMRHRPEAFSINLSPQDLMSERTLQHLEAAIKSVADPARVGLEVLESEQIKD
YGRMIEVCNHFRTLGATIIVDDFGSGYSNIDEIVKLEPQVIKLDGSLIRNIDQDVKQRRIAEQLVKLCQVLNAKTVAEFV
HNQTVCRISEDMGVDYLQGYFLGRPSRLG
>Mature_829_residues
MFTVSRLIPDLASANQVLETMDLPHGQSILVQIFSPLSREHVVQLARLIRSRHPQACLLGCSTEEVIFQGEVHHQVTLLQ
ITVFEQTYLSRAVVDYSDDEAADAERLARQLELTSMSRAVVCFSWQMDTLQVARFALRDTQGAPVPVAGGAAKQTPSGRW
VLLDEACYQNASVAIALHGEALYVETGGYTEWQPVGRTYRVTAVEGDRVLRLDDEPIEAIYQRNLGAQADLPHDWLISFP
LMKGECRHQDLYLPLGLAEEGGLRFNRPLALQDEVRFCFDHPSLTLERVYLTAQQLQAKQCQQVWVFNCALRLNFMHENH
ELQPLQAVAPTDGCYCWGELLYEHGQQQVMHHSMTFLALREGAVRDDLVPIPLPSYPEGMTSPLFNLIRHAFHDLDAMTD
NLAQQIRAQTSLLTASYRRDRRTGLPNRVVLRERLANFAANEHLIALKVTNFNQINEKYGYPVGDKLLRDLSEQFQVFLD
QKLAGQSGLYAIGVGEWATVFRAKLDGKSIHSHFYQFVEQLEHVNFEPYGLPNVDYLSISLCAGLVSQGDFAEHSPDELL
LRAIEARRYAFNNNHHFCNAARLKVQESVRQERLNWLSRVSRAVVRDDVVVYAQPICQARSHIVASYECLVRIEDEGEII
LPGNFLPIITDTHLYTRLSRQMITHTFNMMRHRPEAFSINLSPQDLMSERTLQHLEAAIKSVADPARVGLEVLESEQIKD
YGRMIEVCNHFRTLGATIIVDDFGSGYSNIDEIVKLEPQVIKLDGSLIRNIDQDVKQRRIAEQLVKLCQVLNAKTVAEFV
HNQTVCRISEDMGVDYLQGYFLGRPSRLG

Specific function: Unknown

COG id: COG3287

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI87082096, Length=240, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788849, Length=245, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1790496, Length=243, Percent_Identity=27.1604938271605, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787541, Length=445, Percent_Identity=24.9438202247191, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87081743, Length=233, Percent_Identity=26.1802575107296, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI87081980, Length=241, Percent_Identity=25.7261410788382, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081921, Length=427, Percent_Identity=24.1217798594848, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI226510982, Length=421, Percent_Identity=22.8028503562945, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI87081845, Length=232, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788502, Length=239, Percent_Identity=25.1046025104602, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786507, Length=283, Percent_Identity=22.6148409893993, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1787055, Length=326, Percent_Identity=25.1533742331288, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 94056; Mature: 94056

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTVSRLIPDLASANQVLETMDLPHGQSILVQIFSPLSREHVVQLARLIRSRHPQACLLG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEC
CSTEEVIFQGEVHHQVTLLQITVFEQTYLSRAVVDYSDDEAADAERLARQLELTSMSRAV
CCCHHEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEE
VCFSWQMDTLQVARFALRDTQGAPVPVAGGAAKQTPSGRWVLLDEACYQNASVAIALHGE
EEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCEEEEEECC
ALYVETGGYTEWQPVGRTYRVTAVEGDRVLRLDDEPIEAIYQRNLGAQADLPHDWLISFP
EEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCHHHHEECC
LMKGECRHQDLYLPLGLAEEGGLRFNRPLALQDEVRFCFDHPSLTLERVYLTAQQLQAKQ
CCCCCCCCCCEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
CQQVWVFNCALRLNFMHENHELQPLQAVAPTDGCYCWGELLYEHGQQQVMHHSMTFLALR
HHHHHHHEEEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
EGAVRDDLVPIPLPSYPEGMTSPLFNLIRHAFHDLDAMTDNLAQQIRAQTSLLTASYRRD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTGLPNRVVLRERLANFAANEHLIALKVTNFNQINEKYGYPVGDKLLRDLSEQFQVFLD
HHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QKLAGQSGLYAIGVGEWATVFRAKLDGKSIHSHFYQFVEQLEHVNFEPYGLPNVDYLSIS
HHHCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LCAGLVSQGDFAEHSPDELLLRAIEARRYAFNNNHHFCNAARLKVQESVRQERLNWLSRV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
SRAVVRDDVVVYAQPICQARSHIVASYECLVRIEDEGEIILPGNFLPIITDTHLYTRLSR
HHHHHHCCHHEEEHHHHHHHHHHHEEEEEEEEECCCCCEEECCCEEEEEECHHHHHHHHH
QMITHTFNMMRHRPEAFSINLSPQDLMSERTLQHLEAAIKSVADPARVGLEVLESEQIKD
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YGRMIEVCNHFRTLGATIIVDDFGSGYSNIDEIVKLEPQVIKLDGSLIRNIDQDVKQRRI
HHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
AEQLVKLCQVLNAKTVAEFVHNQTVCRISEDMGVDYLQGYFLGRPSRLG
HHHHHHHHHHHCHHHHHHHHCCCCEEEECHHCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFTVSRLIPDLASANQVLETMDLPHGQSILVQIFSPLSREHVVQLARLIRSRHPQACLLG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEC
CSTEEVIFQGEVHHQVTLLQITVFEQTYLSRAVVDYSDDEAADAERLARQLELTSMSRAV
CCCHHEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEE
VCFSWQMDTLQVARFALRDTQGAPVPVAGGAAKQTPSGRWVLLDEACYQNASVAIALHGE
EEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCEEEEEECC
ALYVETGGYTEWQPVGRTYRVTAVEGDRVLRLDDEPIEAIYQRNLGAQADLPHDWLISFP
EEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHCCCCCCCCCHHHHEECC
LMKGECRHQDLYLPLGLAEEGGLRFNRPLALQDEVRFCFDHPSLTLERVYLTAQQLQAKQ
CCCCCCCCCCEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
CQQVWVFNCALRLNFMHENHELQPLQAVAPTDGCYCWGELLYEHGQQQVMHHSMTFLALR
HHHHHHHEEEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
EGAVRDDLVPIPLPSYPEGMTSPLFNLIRHAFHDLDAMTDNLAQQIRAQTSLLTASYRRD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRTGLPNRVVLRERLANFAANEHLIALKVTNFNQINEKYGYPVGDKLLRDLSEQFQVFLD
HHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QKLAGQSGLYAIGVGEWATVFRAKLDGKSIHSHFYQFVEQLEHVNFEPYGLPNVDYLSIS
HHHCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LCAGLVSQGDFAEHSPDELLLRAIEARRYAFNNNHHFCNAARLKVQESVRQERLNWLSRV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
SRAVVRDDVVVYAQPICQARSHIVASYECLVRIEDEGEIILPGNFLPIITDTHLYTRLSR
HHHHHHCCHHEEEHHHHHHHHHHHEEEEEEEEECCCCCEEECCCEEEEEECHHHHHHHHH
QMITHTFNMMRHRPEAFSINLSPQDLMSERTLQHLEAAIKSVADPARVGLEVLESEQIKD
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YGRMIEVCNHFRTLGATIIVDDFGSGYSNIDEIVKLEPQVIKLDGSLIRNIDQDVKQRRI
HHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
AEQLVKLCQVLNAKTVAEFVHNQTVCRISEDMGVDYLQGYFLGRPSRLG
HHHHHHHHHHHCHHHHHHHHCCCCEEEECHHCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]