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The map label for this gene is ubiE

Identifier: 227080319

GI number: 227080319

Start: 81628

End: 82410

Strand: Direct

Name: ubiE

Synonym: VCM66_0083

Alternate gene names: 227080319

Gene position: 81628-82410 (Clockwise)

Preceding gene: 227080318

Following gene: 227080320

Centisome position: 2.82

GC content: 49.55

Gene sequence:

>783_bases
ATGACGGACACCAACGTGCTAGCAAACTCTGCAACAGACAATCAGGAAACGACCCACTTTGGGTTTGAAACCGTACGTAA
AGATGAAAAAGTGCATAAAGTCGCGCAAGTGTTTCACTCGGTTGCGGCCAAATACGACATCATGAATGACCTGATGTCGG
GCGGTATCCACCGTTTATGGAAGCGCTTTACCATCGATTGTTCAGGCGCACGTCCGGGACAACGCATTCTCGATTTGGGC
GGCGGTACAGGCGATTTAACCGCGAAATTCTCGCGTATTGTGGGCGAGAAAGGCCATGTGATTTTGGCCGATATCAATAA
CTCTATGCTCAATGTTGGCCGCGATAAACTGCGCGACAGCGGCGTGGTCGGTAATGTGCATTACGTGCAAGCCAACGCCG
AAGAACTGCCTTTCCCAGACAACTATTTTGACTGCATCACCATCAGTTTCTGTCTGCGTAACGTGACCGACAAAGATAAA
GCGCTGCGTTCGATGTTCCGTGTATTGAAACCCGGCGGCCGCCTGTTGGTGCTGGAGTTTTCTAAGCCGATCCTTGAGCC
GCTGTCTAAGCTTTATGACACTTACTCGTTCCATATTTTGCCTAAGATGGGGCAACTGATTGCCAATGATGCAGACAGCT
ATCGCTATCTTGCTGAGTCGATCCGCATGCATCCGGATCAAGAGACTCTAAAAGGCATGATGGAAGAGGCGGGTTTTGAA
CAAACCACTTATTACAACCTGACCGGCGGCATTGTGGCGCTGCATCGTGGTTACAAATTTTAA

Upstream 100 bases:

>100_bases
ATCAGGAAAATGCCCCTTTAGTAGAAAGACATCCGATTGAGGATAAAGTAAACTAATCGCCCGCAGTCGCCGCAGTCATG
ACGGCCAAAGAGGAAAAATA

Downstream 100 bases:

>100_bases
GGGCTGCTATGTCATTGCTTGCAACCATGCCACTGTCTCCTTTGCTGACCGCGGTGATTGAAACCACGCTCAATACGCTC
ATCAACGATGATCCGGCGTT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTDTNVLANSATDNQETTHFGFETVRKDEKVHKVAQVFHSVAAKYDIMNDLMSGGIHRLWKRFTIDCSGARPGQRILDLG
GGTGDLTAKFSRIVGEKGHVILADINNSMLNVGRDKLRDSGVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTDKDK
ALRSMFRVLKPGGRLLVLEFSKPILEPLSKLYDTYSFHILPKMGQLIANDADSYRYLAESIRMHPDQETLKGMMEEAGFE
QTTYYNLTGGIVALHRGYKF

Sequences:

>Translated_260_residues
MTDTNVLANSATDNQETTHFGFETVRKDEKVHKVAQVFHSVAAKYDIMNDLMSGGIHRLWKRFTIDCSGARPGQRILDLG
GGTGDLTAKFSRIVGEKGHVILADINNSMLNVGRDKLRDSGVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTDKDK
ALRSMFRVLKPGGRLLVLEFSKPILEPLSKLYDTYSFHILPKMGQLIANDADSYRYLAESIRMHPDQETLKGMMEEAGFE
QTTYYNLTGGIVALHRGYKF
>Mature_259_residues
TDTNVLANSATDNQETTHFGFETVRKDEKVHKVAQVFHSVAAKYDIMNDLMSGGIHRLWKRFTIDCSGARPGQRILDLGG
GTGDLTAKFSRIVGEKGHVILADINNSMLNVGRDKLRDSGVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTDKDKA
LRSMFRVLKPGGRLLVLEFSKPILEPLSKLYDTYSFHILPKMGQLIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQ
TTYYNLTGGIVALHRGYKF

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=45.6521739130435, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI48994983, Length=247, Percent_Identity=79.7570850202429, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557039, Length=254, Percent_Identity=42.9133858267717, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323526, Length=260, Percent_Identity=43.0769230769231, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24641702, Length=266, Percent_Identity=46.6165413533835, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_VIBC3 (A5F4E5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4E5
- SMR:   A5F4E5
- STRING:   A5F4E5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2432
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   VCHO345073:VC0395_A2432-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 29218; Mature: 29087

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTNVLANSATDNQETTHFGFETVRKDEKVHKVAQVFHSVAAKYDIMNDLMSGGIHRLW
CCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRFTIDCSGARPGQRILDLGGGTGDLTAKFSRIVGEKGHVILADINNSMLNVGRDKLRDS
HHHEEECCCCCCCHHHEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHHCC
GVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTDKDKALRSMFRVLKPGGRLLVLEF
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEC
SKPILEPLSKLYDTYSFHILPKMGQLIANDADSYRYLAESIRMHPDQETLKGMMEEAGFE
CCHHHHHHHHHHHHHHEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
QTTYYNLTGGIVALHRGYKF
CCEEEECCCCEEEECCCCCC
>Mature Secondary Structure 
TDTNVLANSATDNQETTHFGFETVRKDEKVHKVAQVFHSVAAKYDIMNDLMSGGIHRLW
CCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRFTIDCSGARPGQRILDLGGGTGDLTAKFSRIVGEKGHVILADINNSMLNVGRDKLRDS
HHHEEECCCCCCCHHHEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHHCC
GVVGNVHYVQANAEELPFPDNYFDCITISFCLRNVTDKDKALRSMFRVLKPGGRLLVLEF
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEC
SKPILEPLSKLYDTYSFHILPKMGQLIANDADSYRYLAESIRMHPDQETLKGMMEEAGFE
CCHHHHHHHHHHHHHHEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
QTTYYNLTGGIVALHRGYKF
CCEEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA