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The map label for this gene is epsC [H]

Identifier: 226950539

GI number: 226950539

Start: 3538373

End: 3540238

Strand: Reverse

Name: epsC [H]

Synonym: CLM_3515

Alternate gene names: 226950539

Gene position: 3540238-3538373 (Counterclockwise)

Preceding gene: 226950540

Following gene: 226950538

Centisome position: 85.2

GC content: 27.38

Gene sequence:

>1866_bases
ATGAGAAAAGATAGGGAATTGATTCTTTATGATATATTATTAATAATTTTATCATTATATTTAGCAATTTTATTAAGATT
CGATTTTGGTATGGCTTCTATGGCTTCGGAATTTAAAAAATATATGATATTTTTTAAGTTATCAGTAATTCCAGTTATAA
TAATAACCTTATTTTTTAATAAAATATTTAATCTCTATAGTAGTATATGGAAATATGCTTCAGTAGAAGAACTTATGTCT
ATAATTTATTCAGTTTCTATATCTAATATTATATTCATAATTTATAGTTATTTTATTAATTATAAAATTCTAGAAAGTAA
TTATTATAGATTCCCATTTACTGTACATATAATATTTTGGATTCTATCTATAGTATCCCTAGGAGGTACAAGGTTTATAT
ATAGAGTTATAGAAGATAAAGAATCTAATAAGAACAAATGTAACTGTGATTCTAAAGAAAAGAAATTACTCATAATAGGG
GCGGGGGATGCAGCAGCTCTTATTATAAAAGAGATAAAAAGAAATAAGGAACTAAATTATAATATAATAGGTCTTATAGA
TGATGATATAGAAAAGCTAGGCAAGAGAATAAATGGTATACCTGTTTTAGGTGGAAGAGAAAGTATTATACGTATGTCTA
AAAATAAAAAAATAGATGAAATAATATTGGCTATACCTTCGGCATCATCTGCAACAAAAAGCGAAATAGTGTCTATATGT
AAAGAAACTAGTTGTAAATTAAAAACATTACCAAGCATGGATAAGGTAATACAGGGCAAGTTTAATATGAGTAAGTTAAG
AGATGTAAGTATTGAGGATTTGCTTGGAAGAGAAGAAGTTAAATTAGATGATAGTTGTATAAACAAATATATAAAGGATA
AAGTGGTTTTAGTTACTGGTGGAGGGGGATCTATTGGGTCAGAACTTTGTAGACAAATAGTTAAATTTTCACCTAAAAAG
CTTTTAATATTAGATATATATGAAAACAATGTCTATGATGTTCAAATGGAACTAAATTATAAATATCCAGAACTGGACAA
AGCTGTTATAATAGCTTCTATAAGGGATGAAAAGAGACTAAAAGATATATTTTCATTATATAAACCAGATGTAGTTTTTC
ATGCAGCAGCTCATAAGCATGTTCCACTAATGGAAGAGAATCCAGCAGAATCTATTAAAAATAATGTAATAGGTACCTAT
AATTTAATAAAATGTGCCCATGAATTTGGGATTGAGCGTTTCGTACAAATAAGTACAGACAAAGCTGTTAATCCAACTAA
TATAATGGGAGCTACAAAAAGATTTTGTGAAATAATGATTCAGGCATTTGATAAAATAAGTAAAACTGAGTTTGTAGCAG
TTAGATTTGGGAATGTTTTAGGAAGTAATGGATCTGTTATTCCATTATTTAAAAAACAAATAACTCATGGAGGTCCTGTT
ACTGTAACTCATCCAGAAATAAATAGATATTTTATGACTATACCAGAAGCAGCTCAATTAGTTATACAAGCAGGAGCTAT
GGCAAAAGGTGGAGAAATATTTGTTTTGGATATGGGAAAGCCAGTTAAAATTGTAGATTTAGCTAAAGATTTAATAACCT
TATCTGGGTATAAGCCAGGGGAGGATATTAAAATTGAGTATACAGGATTAAGACCTGGGGAAAAATTATATGAAGAATTA
TTAATGGATGAAATAGCTTTAACATCAACAGGACATAATAAAATATTTGTAGAAAAGCCTATGGAAAATAATACAGACTT
TGTAGAAAAATCCGTAGAAGAATTTAAAAAAGTTGTTTATAATGATAAAGAAGAAATATTTGCATTGATAGAAAAGAAGG
TTCCAACATATATAAGAAAAAAATAA

Upstream 100 bases:

>100_bases
GTATATATGATAAAATAAAGATATTGCAAAAAATTGTTGAATAATTTTATCAATGATGTAAAATAAATAAGTAGAGTTTA
GAGTTTAAGGAAGTGATTAT

Downstream 100 bases:

>100_bases
TGCTTAACAAATAATAGGTAAGTTTTATTTACAAAATTTAAATATAAAATAATAGTAGTTATGAAATAGTGACAAAAAAT
TAAAAATATATAAATTAAGC

Product: exopolysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIITLFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKESNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYNIIGLIDDDIEKLGKRINGIPVLGGRESIIRMSKNKKIDEIILAIPSASSATKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSCINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNVYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQITHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNTDFVEKSVEEFKKVVYNDKEEIFALIEKKVPTYIRKK

Sequences:

>Translated_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIITLFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKESNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYNIIGLIDDDIEKLGKRINGIPVLGGRESIIRMSKNKKIDEIILAIPSASSATKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSCINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNVYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQITHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNTDFVEKSVEEFKKVVYNDKEEIFALIEKKVPTYIRKK
>Mature_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIITLFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKESNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYNIIGLIDDDIEKLGKRINGIPVLGGRESIIRMSKNKKIDEIILAIPSASSATKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSCINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNVYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQITHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNTDFVEKSVEEFKKVVYNDKEEIFALIEKKVPTYIRKK

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=265, Percent_Identity=29.0566037735849, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI48994969, Length=140, Percent_Identity=30.7142857142857, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 70637; Mature: 70637

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIITLFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKESNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNKELNY
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCE
NIIGLIDDDIEKLGKRINGIPVLGGRESIIRMSKNKKIDEIILAIPSASSATKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSCINKYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNVYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEECCCCCCCCEEEEEECCCHHHH
KDIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGIERFVQISTD
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQITHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEEHHHHHCCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPG
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNTDFVEKSVEEFKKVVY
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NDKEEIFALIEKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIITLFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKESNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNKELNY
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCE
NIIGLIDDDIEKLGKRINGIPVLGGRESIIRMSKNKKIDEIILAIPSASSATKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSCINKYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNVYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEECCCCCCCCEEEEEECCCHHHH
KDIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGIERFVQISTD
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQITHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEEHHHHHCCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPG
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNTDFVEKSVEEFKKVVY
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NDKEEIFALIEKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]