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The map label for this gene is hisS [H]

Identifier: 226950484

GI number: 226950484

Start: 3481135

End: 3482382

Strand: Reverse

Name: hisS [H]

Synonym: CLM_3460

Alternate gene names: 226950484

Gene position: 3482382-3481135 (Counterclockwise)

Preceding gene: 226950485

Following gene: 226950483

Centisome position: 83.81

GC content: 29.73

Gene sequence:

>1248_bases
ATGTCATTGCAAGCGCCAAAAGGTACAAAAGATTTATTGCCTACGGAATCATATAAGTGGCAATATTTAGAGAATAAATT
TAGAAATATAGCAGCAGATTTTGGCTGTAGAGAAATAAGAACTCCAGTTTTTGAATATACAGAATTATTTCAAAGAGGAG
TAGGGGAAACTACAGATGTAGTTCAAAAGGAAATGTATACTTTTGAAGATAAAGCTGGTAGAAGTATAACTTTAAAGCCA
GAAGGAACATCACCAGCAGTAAGAGCCTTCGTTGAAGGAAGATTATTCAATGAAACTCAACCTACTAAGATGTATTATTT
TACACCTGTTATGAGATATGAGAATGTTCAAAAGGGAAGACTTAGACAACATCATCAATTCGGAATAGAAATTTTTGGAG
CAAAAGATGCATCTGTGGATGCAGAAGTTATAAGTATACCTGTTGGAATATATAAAGAATTAGGTGTAGAGGGTGTAGAA
TTAAATATAAATAGCATAGGTTGCCCTAAATGTAGAAAAACTTATAATGAAGCACTAAAAAAATATCTATCTAAAAATTA
TGATAAATTATGCTCAACTTGTAAAACTAGATTTGATAAAAATCCACTTAGAATATTAGATTGCAAAGTTGATACTTGTA
AAGAAATAGTAAAAGATGCACCAATTATATTAGATTATATATGTGGTGAATGTAAGGAGCACTTTGAATCATTAAAAAAT
TATTTAGATGTGTTAAATATAAATTATAAAATAGATCCATTTATAGTTAGAGGATTAGACTATTATAGTAAAACCGTATT
TGAATTTATAACAGATGATATAACTATATGCGCTGGAGGAAGATATGACTATCTTATAGAAGAAATAGGTGGGCCTTCTA
TGCCAGCAGTAGGATTTGGAATGGGGATGGAAAGATTACTTTTAACATTACAAGAAAAAGCAATAGAAATTCCTGAAGAA
GCATATGTAGATTTATATTTAGGAAATATGGGAGATAAAGCTAAATTAGAGGTTTTAAAATTAGCTAAAGAATTAAGGGA
TAGACATATAAAGTGTGAAATAGACCATATGGGCAAAAGTGTTAAAGCCCAAATGAAGTATGCAAATAGAATAGGTGCTA
AATATTCTATGGTATTAGGTGAAGAAGAATTAAATACTGGCAAGGCAACAATTAAAAAAATGGAAGATGGACAGCAAATA
GAAGTGGATATAAAAGATATGGATACATTAATTAAAGTATTTAAATAG

Upstream 100 bases:

>100_bases
TAAGGATGTAATAGAATATACAAAAAGAGTAGAAGAAATGGTACATAGAAAGAAAAAAATATTAGATACTTTATATTTAT
AATGGTTAGGAGGAATATAA

Downstream 100 bases:

>100_bases
ACAAAATAAAATAACAAATCTATATTTGGAGGAATAAAAATGGGAGAAGCATTAAGAGGACTTAAGAGAACAATAATGTG
TGGCGAGCCTAGAGAAAACA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICGECKEHFESLKN
YLDVLNINYKIDPFIVRGLDYYSKTVFEFITDDITICAGGRYDYLIEEIGGPSMPAVGFGMGMERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKATIKKMEDGQQI
EVDIKDMDTLIKVFK

Sequences:

>Translated_415_residues
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKP
EGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVE
LNINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICGECKEHFESLKN
YLDVLNINYKIDPFIVRGLDYYSKTVFEFITDDITICAGGRYDYLIEEIGGPSMPAVGFGMGMERLLLTLQEKAIEIPEE
AYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKATIKKMEDGQQI
EVDIKDMDTLIKVFK
>Mature_414_residues
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDVVQKEMYTFEDKAGRSITLKPE
GTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGRLRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVEL
NINSIGCPKCRKTYNEALKKYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICGECKEHFESLKNY
LDVLNINYKIDPFIVRGLDYYSKTVFEFITDDITICAGGRYDYLIEEIGGPSMPAVGFGMGMERLLLTLQEKAIEIPEEA
YVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKSVKAQMKYANRIGAKYSMVLGEEELNTGKATIKKMEDGQQIE
VDIKDMDTLIKVFK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=459, Percent_Identity=24.6187363834423, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788861, Length=409, Percent_Identity=43.0317848410758, Blast_Score=348, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=24.2616033755274, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47473; Mature: 47342

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICGECKEHFESLKN
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLNINYKIDPFIVRGLDYYSKTVFEFITDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHCCCEEECHHEEECHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCHHHH
MGMERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
CCHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKATIKKMEDGQQIEVDIKDMDTLIKVFK
HHHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
SLQAPKGTKDLLPTESYKWQYLENKFRNIAADFGCREIRTPVFEYTELFQRGVGETTDV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHCCCCHHHH
VQKEMYTFEDKAGRSITLKPEGTSPAVRAFVEGRLFNETQPTKMYYFTPVMRYENVQKGR
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCCHHHH
LRQHHQFGIEIFGAKDASVDAEVISIPVGIYKELGVEGVELNINSIGCPKCRKTYNEALK
HHHHHHCCEEEEECCCCCCCCCCEEECHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KYLSKNYDKLCSTCKTRFDKNPLRILDCKVDTCKEIVKDAPIILDYICGECKEHFESLKN
HHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YLDVLNINYKIDPFIVRGLDYYSKTVFEFITDDITICAGGRYDYLIEEIGGPSMPAVGFG
HHHHHCCCEEECHHEEECHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCHHHH
MGMERLLLTLQEKAIEIPEEAYVDLYLGNMGDKAKLEVLKLAKELRDRHIKCEIDHMGKS
CCHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHCCHH
VKAQMKYANRIGAKYSMVLGEEELNTGKATIKKMEDGQQIEVDIKDMDTLIKVFK
HHHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA