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The map label for this gene is algI [H]

Identifier: 226950475

GI number: 226950475

Start: 3474078

End: 3475565

Strand: Direct

Name: algI [H]

Synonym: CLM_3451

Alternate gene names: 226950475

Gene position: 3474078-3475565 (Clockwise)

Preceding gene: 226950474

Following gene: 226950476

Centisome position: 83.61

GC content: 24.87

Gene sequence:

>1488_bases
TTGTTATTTAACTCGATGCATTTTTTACTGTTTTTCCCAATTGTTACTTTTATATATTTTTTTATACCTCATAAATTTCG
TTATATCTGGTTACTTATAACTAGTTATTATTTTTATATGAGTTGGAATCCTAAATATGCATTATTAATTGGTATTTCAA
CAATAATTACATATTTAAGTGGTATATTAATAGAAAAATCAAATAAAATAACTGATAAACAAAAATCAAAATTTTTTAAA
AAACTTTGGGTTTTTTTAAGCTTATTTATTAATTTAAGCATATTGTTTTTATTTAAATATTGCAATTTTTTCACATATAC
CCTTACAAAAATATTTTCCTTAATTAATATTACAATTAAAGTACCCTCCTTTGACTTTATATTACCTGTAGGAATCTCTT
TCTACACATTCCAGGCTCTTAGCTATACTATAGATGTCTACAGAGGTAATATTAAATTTGAAAAAAATCTAGGCAAATAT
GCTTTGTTTGTGTCATTTTTTCCTCAATTAGTGGCTGGACCTATAGAAAAATCAAAAGATTTATTGAATCAATTTAATGA
AAAACATATATTTGATTATAATAGAGTAAAAAATGGTTTAGTACTAATGCTATGGGGATTTTTCCAAAAGGTATTCGTTT
CTGATAGATTAGCTATTCTAGTAGATACTGTATTTAATAACCCTAGTAATTATAAAGGTTTAGAAATTATTGTTGCATCT
GTTTTTTTTGCCATTCAAATCTATTGTGACTTTTCTTCTTATTCAAATATAGCTAGAGGTGCTGCTGAAGTTATGGGATT
TGATTTATCACTAAACTTTAAACAACCTTATTTTTCAAAATCAATACAAGAATTTTGGAGACGTTGGCACATTACTCTCG
GAGCTTGGTTTAAGGATTACTTATATATACCTTTAGGTGGAAATAAATGCAGTAAATGGAGAAGATATTTTAATAATATG
GTTGTATTCTTAATAAGTGGATTATGGCATGGAGCAGCTATAAATTTTATAATCTGGGGTGGCTTACATGGATTCTACAT
AATAATAGGAGATATGCTAAAACCACTTAAAGAAAAAATAATAAGTAATTTAAAGATAAAAACTAATGTGTTTAGCTTTA
AATTATTCCAAACATTGTTTACTTTCATATTAGTTGATTTTTCATGGATATTTTTTAGAGCAAATTCATTTTCTGAAGCT
AAACTATTAATTAAAAATATGTTTTATTTTAATCCATGGATATTTACCTATGGTTCCATCTATAAATTAGGATTAGATCA
AAAAGATTTTAAAATGTCAATACTAGGTATATTAATAGTTTTTATTATAGATAGTATTGCAAGAAATAAAGATGTACGTG
TTGAACTTTCTAATCAAAATATAGTATTTAGATGGGCTATTTATTTATCTGCTATTTTCTTTGTGCTAATATTAGGTATA
TATGGAGCAGGGTATGATGCAAATCAATTTATTTATTTTCAGTTTTAA

Upstream 100 bases:

>100_bases
AAGGAGGAGAACAGCTATAAAAATTGTTTTTAAAAAGTTGATGTAATCTAGTATTTATCATATTTGGATCATGAATTAAT
TAAGAAGGAGTGTATGGTAA

Downstream 100 bases:

>100_bases
GGAGAATTAGTTATGAGAAAAAAATTTATATTTAGATTTACTACTTTTATAATAATACTATTGCTTATTATATCTATAAT
AACTCCTGCATTTATAATAA

Product: alginate O-acetyltransferase AlgI

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MLFNSMHFLLFFPIVTFIYFFIPHKFRYIWLLITSYYFYMSWNPKYALLIGISTIITYLSGILIEKSNKITDKQKSKFFK
KLWVFLSLFINLSILFLFKYCNFFTYTLTKIFSLINITIKVPSFDFILPVGISFYTFQALSYTIDVYRGNIKFEKNLGKY
ALFVSFFPQLVAGPIEKSKDLLNQFNEKHIFDYNRVKNGLVLMLWGFFQKVFVSDRLAILVDTVFNNPSNYKGLEIIVAS
VFFAIQIYCDFSSYSNIARGAAEVMGFDLSLNFKQPYFSKSIQEFWRRWHITLGAWFKDYLYIPLGGNKCSKWRRYFNNM
VVFLISGLWHGAAINFIIWGGLHGFYIIIGDMLKPLKEKIISNLKIKTNVFSFKLFQTLFTFILVDFSWIFFRANSFSEA
KLLIKNMFYFNPWIFTYGSIYKLGLDQKDFKMSILGILIVFIIDSIARNKDVRVELSNQNIVFRWAIYLSAIFFVLILGI
YGAGYDANQFIYFQF

Sequences:

>Translated_495_residues
MLFNSMHFLLFFPIVTFIYFFIPHKFRYIWLLITSYYFYMSWNPKYALLIGISTIITYLSGILIEKSNKITDKQKSKFFK
KLWVFLSLFINLSILFLFKYCNFFTYTLTKIFSLINITIKVPSFDFILPVGISFYTFQALSYTIDVYRGNIKFEKNLGKY
ALFVSFFPQLVAGPIEKSKDLLNQFNEKHIFDYNRVKNGLVLMLWGFFQKVFVSDRLAILVDTVFNNPSNYKGLEIIVAS
VFFAIQIYCDFSSYSNIARGAAEVMGFDLSLNFKQPYFSKSIQEFWRRWHITLGAWFKDYLYIPLGGNKCSKWRRYFNNM
VVFLISGLWHGAAINFIIWGGLHGFYIIIGDMLKPLKEKIISNLKIKTNVFSFKLFQTLFTFILVDFSWIFFRANSFSEA
KLLIKNMFYFNPWIFTYGSIYKLGLDQKDFKMSILGILIVFIIDSIARNKDVRVELSNQNIVFRWAIYLSAIFFVLILGI
YGAGYDANQFIYFQF
>Mature_495_residues
MLFNSMHFLLFFPIVTFIYFFIPHKFRYIWLLITSYYFYMSWNPKYALLIGISTIITYLSGILIEKSNKITDKQKSKFFK
KLWVFLSLFINLSILFLFKYCNFFTYTLTKIFSLINITIKVPSFDFILPVGISFYTFQALSYTIDVYRGNIKFEKNLGKY
ALFVSFFPQLVAGPIEKSKDLLNQFNEKHIFDYNRVKNGLVLMLWGFFQKVFVSDRLAILVDTVFNNPSNYKGLEIIVAS
VFFAIQIYCDFSSYSNIARGAAEVMGFDLSLNFKQPYFSKSIQEFWRRWHITLGAWFKDYLYIPLGGNKCSKWRRYFNNM
VVFLISGLWHGAAINFIIWGGLHGFYIIIGDMLKPLKEKIISNLKIKTNVFSFKLFQTLFTFILVDFSWIFFRANSFSEA
KLLIKNMFYFNPWIFTYGSIYKLGLDQKDFKMSILGILIVFIIDSIARNKDVRVELSNQNIVFRWAIYLSAIFFVLILGI
YGAGYDANQFIYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 58444; Mature: 58444

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSMHFLLFFPIVTFIYFFIPHKFRYIWLLITSYYFYMSWNPKYALLIGISTIITYLS
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHHHHH
GILIEKSNKITDKQKSKFFKKLWVFLSLFINLSILFLFKYCNFFTYTLTKIFSLINITIK
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VPSFDFILPVGISFYTFQALSYTIDVYRGNIKFEKNLGKYALFVSFFPQLVAGPIEKSKD
CCCCCEEEECCCHHHHHHHHHEEEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHH
LLNQFNEKHIFDYNRVKNGLVLMLWGFFQKVFVSDRLAILVDTVFNNPSNYKGLEIIVAS
HHHHCCCCCCCCHHHHCCCCEEHHHHHHHHHHHCCCHHEEEEHHHCCCCCCCHHHHHHHH
VFFAIQIYCDFSSYSNIARGAAEVMGFDLSLNFKQPYFSKSIQEFWRRWHITLGAWFKDY
HHHHHHEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHCE
LYIPLGGNKCSKWRRYFNNMVVFLISGLWHGAAINFIIWGGLHGFYIIIGDMLKPLKEKI
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ISNLKIKTNVFSFKLFQTLFTFILVDFSWIFFRANSFSEAKLLIKNMFYFNPWIFTYGSI
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCHHHHCCCE
YKLGLDQKDFKMSILGILIVFIIDSIARNKDVRVELSNQNIVFRWAIYLSAIFFVLILGI
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
YGAGYDANQFIYFQF
HCCCCCCCEEEEEEC
>Mature Secondary Structure
MLFNSMHFLLFFPIVTFIYFFIPHKFRYIWLLITSYYFYMSWNPKYALLIGISTIITYLS
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHHHHH
GILIEKSNKITDKQKSKFFKKLWVFLSLFINLSILFLFKYCNFFTYTLTKIFSLINITIK
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
VPSFDFILPVGISFYTFQALSYTIDVYRGNIKFEKNLGKYALFVSFFPQLVAGPIEKSKD
CCCCCEEEECCCHHHHHHHHHEEEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHH
LLNQFNEKHIFDYNRVKNGLVLMLWGFFQKVFVSDRLAILVDTVFNNPSNYKGLEIIVAS
HHHHCCCCCCCCHHHHCCCCEEHHHHHHHHHHHCCCHHEEEEHHHCCCCCCCHHHHHHHH
VFFAIQIYCDFSSYSNIARGAAEVMGFDLSLNFKQPYFSKSIQEFWRRWHITLGAWFKDY
HHHHHHEEECCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHCE
LYIPLGGNKCSKWRRYFNNMVVFLISGLWHGAAINFIIWGGLHGFYIIIGDMLKPLKEKI
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ISNLKIKTNVFSFKLFQTLFTFILVDFSWIFFRANSFSEAKLLIKNMFYFNPWIFTYGSI
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCHHHHCCCE
YKLGLDQKDFKMSILGILIVFIIDSIARNKDVRVELSNQNIVFRWAIYLSAIFFVLILGI
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
YGAGYDANQFIYFQF
HCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]