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The map label for this gene is minD [H]

Identifier: 226950421

GI number: 226950421

Start: 3426871

End: 3427668

Strand: Reverse

Name: minD [H]

Synonym: CLM_3393

Alternate gene names: 226950421

Gene position: 3427668-3426871 (Counterclockwise)

Preceding gene: 226950422

Following gene: 226950420

Centisome position: 82.49

GC content: 32.46

Gene sequence:

>798_bases
ATGGGAGAAGTGATAGTTGTTACCTCTGGTAAAGGTGGAGTAGGAAAAACAACCACATCAGCTAATATATCTACAGCTTT
AGCTGCAATGGATAAAAAAGTAGTTGTTATAGATGGAGATACGGGATTAAGAAACCTTGATGTTTTAATGGGGCTTGAAA
ATAGAATAGTATTTACCCTATTAGATGTAATAGAAGAAAGATGTAAGTTAAAACAAGCATTAATAAGGGATAAAAGATTA
AATAGCTTATATCTATTACCAACAGCACAAACAAGAGATAAAGAAGATGTTAATGTGGATGACATGCTTAAAATAGTCAA
TGATTTAAAGCAGGAATTTGATTATGTGATTTTGGATTGTCCAGCAGGCATAGAAAGAGGATTTGAAAGCTCTATAGCAG
GAGCAGATAGAGCTTTAGTAGTTGTAAATCCAGAAGTAACATCTGTAAGAGATGCAGATAGAGTTATAGGGAAATTAGAT
GCTAAAGGCCTAGATAATCATCAGCTTATAGTAAATAGATTAAATTATGAAATGACACAAAGCGGAGATATGTTAGATAT
TGAAGATATAATTGATAGTTTAGCTATAAAGCTTATAGGGGTAGTGCCAGACGATAGAAATATAACTATAGCTACAAATA
AAGGAGAACCTATAGTTTTAGATAATGGAGCTGTAGCTGGACAGGCATTTAGAAACATTGCAAAAAGGATTACTGGAGAA
GAAGTACCTATAATGGATTTAAGAAGTAAGGAACAGGGCTTTTTTAAATCCTTTAAAAAATTATTTGGGTTAAAGTAG

Upstream 100 bases:

>100_bases
TAAAACCCCAATATCCAGAAGTTGCAAAGATTAAATCAAGCATAATAGTAGTAGAACCATATCTACCGGACAAATTTATA
TAAATTAAGGAGGACAAAAA

Downstream 100 bases:

>100_bases
GAGTGATAAGGTAATGGATTTATTTAAGTTTTTTTCAAAACAATCATCAAAAGATGTAGCCAAGGAAAGACTTAAACTTA
TATTAATACAAGATAGGAAC

Product: septum site-determining protein MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MGEVIVVTSGKGGVGKTTTSANISTALAAMDKKVVVIDGDTGLRNLDVLMGLENRIVFTLLDVIEERCKLKQALIRDKRL
NSLYLLPTAQTRDKEDVNVDDMLKIVNDLKQEFDYVILDCPAGIERGFESSIAGADRALVVVNPEVTSVRDADRVIGKLD
AKGLDNHQLIVNRLNYEMTQSGDMLDIEDIIDSLAIKLIGVVPDDRNITIATNKGEPIVLDNGAVAGQAFRNIAKRITGE
EVPIMDLRSKEQGFFKSFKKLFGLK

Sequences:

>Translated_265_residues
MGEVIVVTSGKGGVGKTTTSANISTALAAMDKKVVVIDGDTGLRNLDVLMGLENRIVFTLLDVIEERCKLKQALIRDKRL
NSLYLLPTAQTRDKEDVNVDDMLKIVNDLKQEFDYVILDCPAGIERGFESSIAGADRALVVVNPEVTSVRDADRVIGKLD
AKGLDNHQLIVNRLNYEMTQSGDMLDIEDIIDSLAIKLIGVVPDDRNITIATNKGEPIVLDNGAVAGQAFRNIAKRITGE
EVPIMDLRSKEQGFFKSFKKLFGLK
>Mature_264_residues
GEVIVVTSGKGGVGKTTTSANISTALAAMDKKVVVIDGDTGLRNLDVLMGLENRIVFTLLDVIEERCKLKQALIRDKRLN
SLYLLPTAQTRDKEDVNVDDMLKIVNDLKQEFDYVILDCPAGIERGFESSIAGADRALVVVNPEVTSVRDADRVIGKLDA
KGLDNHQLIVNRLNYEMTQSGDMLDIEDIIDSLAIKLIGVVPDDRNITIATNKGEPIVLDNGAVAGQAFRNIAKRITGEE
VPIMDLRSKEQGFFKSFKKLFGLK

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=272, Percent_Identity=45.9558823529412, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29059; Mature: 28928

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVIVVTSGKGGVGKTTTSANISTALAAMDKKVVVIDGDTGLRNLDVLMGLENRIVFTL
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCHHHHHH
LDVIEERCKLKQALIRDKRLNSLYLLPTAQTRDKEDVNVDDMLKIVNDLKQEFDYVILDC
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEC
PAGIERGFESSIAGADRALVVVNPEVTSVRDADRVIGKLDAKGLDNHQLIVNRLNYEMTQ
CCHHHHCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECC
SGDMLDIEDIIDSLAIKLIGVVPDDRNITIATNKGEPIVLDNGAVAGQAFRNIAKRITGE
CCCEEEHHHHHHHHHHHEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCC
EVPIMDLRSKEQGFFKSFKKLFGLK
CCCEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEVIVVTSGKGGVGKTTTSANISTALAAMDKKVVVIDGDTGLRNLDVLMGLENRIVFTL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHCCCCHHHHHH
LDVIEERCKLKQALIRDKRLNSLYLLPTAQTRDKEDVNVDDMLKIVNDLKQEFDYVILDC
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEC
PAGIERGFESSIAGADRALVVVNPEVTSVRDADRVIGKLDAKGLDNHQLIVNRLNYEMTQ
CCHHHHCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECC
SGDMLDIEDIIDSLAIKLIGVVPDDRNITIATNKGEPIVLDNGAVAGQAFRNIAKRITGE
CCCEEEHHHHHHHHHHHEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCC
EVPIMDLRSKEQGFFKSFKKLFGLK
CCCEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1400225; 8459776; 1400224; 9384377 [H]