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The map label for this gene is purC

Identifier: 226950310

GI number: 226950310

Start: 3298816

End: 3299520

Strand: Reverse

Name: purC

Synonym: CLM_3274

Alternate gene names: 226950310

Gene position: 3299520-3298816 (Counterclockwise)

Preceding gene: 226950311

Following gene: 226950309

Centisome position: 79.41

GC content: 29.22

Gene sequence:

>705_bases
ATGGAAAAGAAGGATATGTTATATGAAGGAAAGGCTAAAAAAATATTCAGAACGGATGATAAGGATACAGTTGTTGTGTA
TTATAAAGATGATGCCACAGCTTTTAATGGGGAAAAGAAAGGAACTATTGAAGATAAGGGAGTTATGAATAACTCTATAA
CGGCAATGTTATTTGAACTTTTAGAAAAAAAGGGCGTAAAAACACATTTTATAGAAAAGATAAATGAAAGAGAACAGCTT
TGCAAAAAAGTAGAAATAGTTCCACTAGAAGTTATAGTTAGAAATATAGCAGCAGGAAGTATGGCAAAAAGATTAGGTCT
TTCAGAAGGAAGAAAATTAGATACTACTGTATTTGAAATAAGTTATAAAAATGATGATTTAAATGACCCTCTTATAAATG
ACTATCATGCAGTGGCTATAGGACTTACAACCTTTGAAGAACTAAAAGAAATGTATTCTATAGCAGAAAAAGTAAATAAT
ACATTAAAAGAATTCTTTGATGAGCAAGGAATAAATTTAGTTGATTTTAAAATAGAAATAGGAAGATTTAATGGTGAGCT
CCTTTTAGCAGATGAAATATCCCCAGATACTTGTAGATTATGGGATAAAAGCACAGGAGAAAAGTTAGATAAAGATAGAT
TTAGAAGAGACATGGGAAATGTAAAAGAAGCTTATATGGAAATATTAAAAAGAGTCAATAAATAG

Upstream 100 bases:

>100_bases
TATCTTTAAAGTATGAAAAGTTAAGAGAAAAACTTATAGAATATAGAAAAAATATGAAAGAAAAATTTATAAATGATAAC
AAAGAGGGAGTGGAGTTATA

Downstream 100 bases:

>100_bases
ATTGTTATAGTTTAAAATTATTTTTACAGCAGCTGGAAATAATCTAAGAGAAGATTAAATTTTTAAAATAGAAAATAAGT
GGGGAGTAAAAATGAGTATG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEKKDMLYEGKAKKIFRTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFEELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK

Sequences:

>Translated_234_residues
MEKKDMLYEGKAKKIFRTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFEELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
>Mature_234_residues
MEKKDMLYEGKAKKIFRTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFEELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=242, Percent_Identity=33.4710743801653, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI5453539, Length=242, Percent_Identity=33.4710743801653, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI119220559, Length=249, Percent_Identity=32.1285140562249, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1788820, Length=232, Percent_Identity=40.5172413793103, Blast_Score=197, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17531275, Length=238, Percent_Identity=34.0336134453782, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18860083, Length=235, Percent_Identity=25.1063829787234, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24583917, Length=250, Percent_Identity=26.8, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_CLOB1 (A7FXD7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385141.1
- ProteinModelPortal:   A7FXD7
- SMR:   A7FXD7
- STRING:   A7FXD7
- GeneID:   5397787
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2842
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- BioCyc:   CBOT441770:CLB_2842-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 26968; Mature: 26968

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKDMLYEGKAKKIFRTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFEL
CCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHH
LEKKGVKTHFIEKINEREQLCKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEI
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SYKNDDLNDPLINDYHAVAIGLTTFEELKEMYSIAEKVNNTLKEFFDEQGINLVDFKIEI
EECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
GRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
EECCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKDMLYEGKAKKIFRTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFEL
CCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHH
LEKKGVKTHFIEKINEREQLCKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEI
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SYKNDDLNDPLINDYHAVAIGLTTFEELKEMYSIAEKVNNTLKEFFDEQGINLVDFKIEI
EECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
GRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
EECCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA