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The map label for this gene is purH [H]

Identifier: 226950306

GI number: 226950306

Start: 3293763

End: 3295262

Strand: Reverse

Name: purH [H]

Synonym: CLM_3270

Alternate gene names: 226950306

Gene position: 3295262-3293763 (Counterclockwise)

Preceding gene: 226950307

Following gene: 226950305

Centisome position: 79.3

GC content: 30.67

Gene sequence:

>1500_bases
GTGATAAAAAGAGCATTAATAAGTGTATTTGATAAAACAGGAATTTTAGATTTGGCAAAGTTTTTAGAAAGTAGAGATGT
AGAAATAATATCTACAGGTGGAACTTATAAACATTTAAAAGAAAATGGAGTAAAAGTAATAGATATAGAAGAGGTAACAG
GTTTCCCAGAAATGTTAGATGGAAGAGTAAAAACATTAAATCCTTTAATTCATGGAGGCATATTAGCTATAAGAGATAAT
GAAGAGCATATGAAGGTAATAGAAGAAAAGGGCATAAACCCTATAGATATGGTAGTAGTGAATTTATATCCTTTCTTTAA
TAAAGTAGAAGAAAATTTAAGTTTTGATGAAAAAGTAGAGTTTATAGACATTGGTGGACCTACTATGATAAGAGCAGCAG
CTAAAAATTTTAAAGATGTAGTAGTACTAACAGATACTAAGGATTATGAAAATGTCATAAATGAGATAAAAGAAAATAAT
CAGGTTAATACACAAACTAGAAAAAAATTAGCAGGAAAAGTTTTCAATCTAATGTCAGCCTATGATGCAGCTATAAGTAA
TTTCTTATTAGAAGAGGAATATCCAGAATATTTAACTCTATCCTATAAAAAGAACATGGATTTAAGATATGGAGAAAATC
CTCATCAAACAGCAGCTTATTACACATCTACTGTTGGAAAATATCCTATGAAAAATTTTGAAAAGTTAAATGGAAAAGAA
TTATCCTACAATAATATAAAGGATATGGATATAGCGTGGAAAACCGTTTGTGAATTTGAAGAGGTGGCTTGTTGTGCATT
AAAACATAATACACCTTGTGGTGTGGCTATAGGGGATACTGTACAGGAAGCTTATACTAAGGCCTATGAATGTGATCCTA
TATCTATATTTGGTGGCATAGTTGCTTTTAATAGAAAAGTAGATAAGGAAACAGCAGAAAATCTTGCAAAAATATTTTTA
GAAATAGTTGTAGCACCAGATTTTGATGAAGATGCTTTAGAAGTCTTAAAAAACAAGAAAAATTTAAGGGTTATAAAATG
TGAAGAAAAATCTACAGAAGGAAAAGATATGGCAAAGGTAGATGGAGGAATATTAGTACAAAAGAGTGATAATAAATTAT
TAGAAAATACAAAAGTAGTTACAGAAAAATCTCCAACAGAGCAAGAAATGAAAGATCTTATATTTGGTATGAAAGTAGTT
AAATATGTGAAATCCAACGCTATTGTAGTAGTTAAAGATGGTATGGCAAAAGGTATTGGGGGAGGCCAAGTAAATAGAAT
ATGGGCAGCAAAGGAAGCATTAGATAGAGCTGGAGATGGCGTAGTTTTAGCTTCAGATGCTTTTTTCCCATTTGGAGATG
TAGCAGAAGAAGCTGCAAAATGGGGAATAAAAGCTATAATTCAACCAGGCGGTTCTATAAGAGATGAAGAATCTATAAAG
GTATGTAATGAAAAAGGGATTTCTATGGTATTTACAGGAATAAGACATTTTAAACACTAG

Upstream 100 bases:

>100_bases
GTGCCACTTATATGTTTTCTTTGAAAGAAGGTATATTGGTGGCAGTTACTTATGACAGATAAATAAGATAATAAAAAATA
TTGAAACTGGGGGAGTTTAA

Downstream 100 bases:

>100_bases
ATTTAAATATTTTTTAGTTAAAGGATAAAAGTCTTAATAAGATATTCTAATACTGAGATGAAAAGAGATATTTTTTATAA
AGAAATTTCATCTCAATATT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNTQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLENTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH

Sequences:

>Translated_499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNTQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLENTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH
>Mature_499_residues
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLDGRVKTLNPLIHGGILAIRDN
EEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVEFIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENN
QVNTQTRKKLAGKVFNLMSAYDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGIVAFNRKVDKETAENLAKIFL
EIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKVDGGILVQKSDNKLLENTKVVTEKSPTEQEMKDLIFGMKVV
KYVKSNAIVVVKDGMAKGIGGGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
VCNEKGISMVFTGIRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=467, Percent_Identity=35.5460385438972, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=46.4761904761905, Blast_Score=473, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71985564, Length=471, Percent_Identity=36.0934182590234, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71985574, Length=324, Percent_Identity=28.7037037037037, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=45, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=467, Percent_Identity=35.3319057815846, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323056, Length=466, Percent_Identity=35.1931330472103, Blast_Score=250, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24649832, Length=469, Percent_Identity=33.6886993603412, Blast_Score=246, Evalue=3e-65,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55735; Mature: 55735

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLD
CCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLNPLIHGGILAIRDNEEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVE
CCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
FIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENNQVNTQTRKKLAGKVFNLMSA
EEECCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCC
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHH
VAFNRKVDKETAENLAKIFLEIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKV
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHCC
DGGILVQKSDNKLLENTKVVTEKSPTEQEMKDLIFGMKVVKYVKSNAIVVVKDGMAKGIG
CCCEEEEECCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VCNEKGISMVFTGIRHFKH
HHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKRALISVFDKTGILDLAKFLESRDVEIISTGGTYKHLKENGVKVIDIEEVTGFPEMLD
CCHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLNPLIHGGILAIRDNEEHMKVIEEKGINPIDMVVVNLYPFFNKVEENLSFDEKVE
CCHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
FIDIGGPTMIRAAAKNFKDVVVLTDTKDYENVINEIKENNQVNTQTRKKLAGKVFNLMSA
EEECCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YDAAISNFLLEEEYPEYLTLSYKKNMDLRYGENPHQTAAYYTSTVGKYPMKNFEKLNGKE
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCC
LSYNNIKDMDIAWKTVCEFEEVACCALKHNTPCGVAIGDTVQEAYTKAYECDPISIFGGI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCHHHHHHH
VAFNRKVDKETAENLAKIFLEIVVAPDFDEDALEVLKNKKNLRVIKCEEKSTEGKDMAKV
HHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHCC
DGGILVQKSDNKLLENTKVVTEKSPTEQEMKDLIFGMKVVKYVKSNAIVVVKDGMAKGIG
CCCEEEEECCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
GGQVNRIWAAKEALDRAGDGVVLASDAFFPFGDVAEEAAKWGIKAIIQPGGSIRDEESIK
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCHHH
VCNEKGISMVFTGIRHFKH
HHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA