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The map label for this gene is rumA [C]

Identifier: 226950293

GI number: 226950293

Start: 3275575

End: 3276927

Strand: Reverse

Name: rumA [C]

Synonym: CLM_3255

Alternate gene names: 226950293

Gene position: 3276927-3275575 (Counterclockwise)

Preceding gene: 226950294

Following gene: 226950292

Centisome position: 78.86

GC content: 30.16

Gene sequence:

>1353_bases
ATGAGAAAAGGCAAGGAGTATGAATTTCTTATAGAAGAAACAGAATTTCCAGGAACAGGTGTAGCACAAAAGGATGGTAT
GCCAGTTTATATAAAAGGAACAGTACCAGGACAAAAGGTTTTAGCAAAAGTAACTAAAAAAAGAAGAGAATATGCTCAGG
CTAAACTTTTAGAAATAATAGAAAATATAGATTATGCAATAGAAAATAAATGTCCTCATTTTGGTCAATGTGGAGGATGT
TCAACCCAATACATACCTTATGAAAAACAACTACAAATTAAAGAAGAACAGCTGTTAAAATTATTTAAAAGTAAAGAAAT
AAAAGGATTTGATTTTTTAGGAGTAGAAAAAAGTCCAGAGGAATACGAATATAGAAATAAGATGGAGTTTACCTTTGGGG
ACATGGAAAAGGGCGGAGATCTTACTTTAGGAATGCATGTTAAAAATAGAAATTTTTCTATTGTAACTGTGGATAAGTGT
GAAATTGTAGATAGAGATTTTAGAAATATATTAACAACTGTGGTTAATTATTTTAATGAAAAAAGATTACCTAAATACAG
AGTAATGAGTCATGAAGGTTTCTTAAGAAACTTAGTTATAAGAAAAGCTAAAAATACTGGAGAAATATTAATAAACATAG
TAACTACATCTCAAATGGAATTTGATTTTAAAGAAATAGTAGATATGTTATTAAAGGTGGAATGCAAAGGAGAAATAAAA
GGCATTCTTCATACTATAAATGATACATTATCTGATGTAGTTCAGGTGGATAAATTAGAAATATTATATGGAAGAGACTA
TATAATAGAAGAGTTATTAGGACTAAAATTTAAGATAGCTCCAGAAGCCTTTTTTCAAACAAATTCAAAGGGAGCAGAAA
AACTATATAGTATAGTAAAAGACTTCTTAGGTGATGCTAGTTCAAAAGTGGTTTTGGACCTTTATTGTGGTACAGGAACC
ATAGGACAAATAGTTGCTCCAAAGGCTAAAAAAGTTATAGGTGTGGAATTAATAGAAGAAGCAGTTAAGGCAGCTAATGA
AAATGCAAAATTAAATGGACTAAATAACTGTGAGTTTATAGCCGGTGATGTAGCAAAGGTTATAAAAGATGTAAAACAAA
AACCAGATATAATTATATTGGATCCACCAAGACCAGGTGTACATCCAGTAGCTTTAGAATATGTGGTTAAGTTTGAGCCA
AAGGAAATAATCTATGTATCTTGTAATCCAAAGACTTTAGTAGATGATTTAAAATATCTTATAGATAATGGGTATAAGCT
AGAAAAAGTTAAGGGCATGGACATGTTTCCTCATACACCTCATGTGGAAACTGTAGTTAAGCTAAGCAAATAA

Upstream 100 bases:

>100_bases
GTAGTTACAGAAAATGGTATTAATAGTATAGCAATAAGATAAAATCTAATAAATAAAATACTTAAAATAAATTTACTCTG
AAAGGATGAAAAATAAATAT

Downstream 100 bases:

>100_bases
TATCAAGGGTATAATTAATTTAAAACTATAAAAATCATTAATAACAGATGAAATTTATAAGATAACAAAAAGAAGTTAGG
AAAAAAACCTAGCTTCTTTT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGMPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVLDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK

Sequences:

>Translated_450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGMPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVLDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK
>Mature_450_residues
MRKGKEYEFLIEETEFPGTGVAQKDGMPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFGQCGGC
STQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKC
EIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVECKGEIK
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVLDLYCGTGT
IGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEP
KEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVETVVKLSK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=356, Percent_Identity=30.3370786516854, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI51173878, Length=356, Percent_Identity=30.3370786516854, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI269784680, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI269784676, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI20149688, Length=364, Percent_Identity=30.2197802197802, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI269784678, Length=358, Percent_Identity=30.1675977653631, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1789148, Length=447, Percent_Identity=28.1879194630872, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=25.6613756613757, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790403, Length=230, Percent_Identity=26.5217391304348, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=551, Percent_Identity=25.9528130671506, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322909, Length=416, Percent_Identity=26.4423076923077, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24666579, Length=428, Percent_Identity=28.9719626168224, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51088; Mature: 51088

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGKEYEFLIEETEFPGTGVAQKDGMPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEII
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPE
HHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCH
EYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKCEIVDRDFRNILTTVVNYFNE
HHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
KRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVECKGEIK
CCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVK
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHHHHHHH
DFLGDASSKVVLDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFI
HHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
AGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVDDLKYL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHHHHHHH
IDNGYKLEKVKGMDMFPHTPHVETVVKLSK
HCCCEEEEEECCCCCCCCCCCHHHHHEECC
>Mature Secondary Structure
MRKGKEYEFLIEETEFPGTGVAQKDGMPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEII
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPE
HHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECCCCCCH
EYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDKCEIVDRDFRNILTTVVNYFNE
HHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
KRLPKYRVMSHEGFLRNLVIRKAKNTGEILINIVTTSQMEFDFKEIVDMLLKVECKGEIK
CCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHH
GILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVK
HHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHHHHHHH
DFLGDASSKVVLDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFI
HHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHH
AGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVDDLKYL
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEEEEEECCCEEEEEECCCHHHHHHHHHH
IDNGYKLEKVKGMDMFPHTPHVETVVKLSK
HCCCEEEEEECCCCCCCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]