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The map label for this gene is p20 [H]

Identifier: 226950285

GI number: 226950285

Start: 3267350

End: 3267889

Strand: Reverse

Name: p20 [H]

Synonym: CLM_3247

Alternate gene names: 226950285

Gene position: 3267889-3267350 (Counterclockwise)

Preceding gene: 226950286

Following gene: 226950284

Centisome position: 78.64

GC content: 32.41

Gene sequence:

>540_bases
ATGTTTGAAAATGAAATCATAAAACTACGAAAATTATCACTTAACGATTACAATATTTATCATAATTGGCGTAACGACGT
AGAGGTCATGTATACCACTAGTCCTCAACTTGACACTTATACTTTAGAAGAAACGGAAGAATTCATTTCATCAATAATTT
CTCAATCTAATGCAAAAAGCTATATAATTGAGCATAAAAAGACTGATCAAGCGGTCGGTATTGTTTCTCTAAGTAATATT
AATTATAAAGACCGTTCTGCTGAATGTATTATTGATATTGGTGTAAAAGATATGTGGGGAAAAGGTATTGGGACAGCAGC
GCTCTCTCTAATACTAGAGTTTGCATTTAGCGAACTTAACTTACATCGAATATCCCTAAAGGTGCTCTCATTCAATGAAA
GAGCAATTAAACTTTATGAGAAAATGGGATTTATACGCGAAGGAATATTACGTCAGGCATTATATCGTGCTGGAAATTGG
CATGATATCGTCGTAATGAGTATTTTAAAGAACGAATATCTATCTGTTAGCTATAAATAA

Upstream 100 bases:

>100_bases
CAACAATATAATCAATTTTGTCCCAGTATTAAACTTTCTAAAATATTTGATTTACTGAATAAGATGATTGAGAGTGTTCT
ATCTTAAGGAGGAGCATTTA

Downstream 100 bases:

>100_bases
GCTCACATAGGAAGTCGTAGGCAAAATTTGCCTTTTAAAAGAAACTGTAGACCATGAAGAAGTTTGGGGAATCGGATATA
TTTTGATTAGAAAATATTAT

Product: acetyltransferase

Products: Coenzyme A; H+; N8-Acetylspermidine [C]

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MFENEIIKLRKLSLNDYNIYHNWRNDVEVMYTTSPQLDTYTLEETEEFISSIISQSNAKSYIIEHKKTDQAVGIVSLSNI
NYKDRSAECIIDIGVKDMWGKGIGTAALSLILEFAFSELNLHRISLKVLSFNERAIKLYEKMGFIREGILRQALYRAGNW
HDIVVMSILKNEYLSVSYK

Sequences:

>Translated_179_residues
MFENEIIKLRKLSLNDYNIYHNWRNDVEVMYTTSPQLDTYTLEETEEFISSIISQSNAKSYIIEHKKTDQAVGIVSLSNI
NYKDRSAECIIDIGVKDMWGKGIGTAALSLILEFAFSELNLHRISLKVLSFNERAIKLYEKMGFIREGILRQALYRAGNW
HDIVVMSILKNEYLSVSYK
>Mature_179_residues
MFENEIIKLRKLSLNDYNIYHNWRNDVEVMYTTSPQLDTYTLEETEEFISSIISQSNAKSYIIEHKKTDQAVGIVSLSNI
NYKDRSAECIIDIGVKDMWGKGIGTAALSLILEFAFSELNLHRISLKVLSFNERAIKLYEKMGFIREGILRQALYRAGNW
HDIVVMSILKNEYLSVSYK

Specific function: Regulates Polyamine Concentration By Their Degradation. Acts On Spermidine, Forming N(1)- And N(8)-Acetylspermidine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787867, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: 2.3.1.57 [C]

Molecular weight: Translated: 20741; Mature: 20741

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENEIIKLRKLSLNDYNIYHNWRNDVEVMYTTSPQLDTYTLEETEEFISSIISQSNAKS
CCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCC
YIIEHKKTDQAVGIVSLSNINYKDRSAECIIDIGVKDMWGKGIGTAALSLILEFAFSELN
EEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHCC
LHRISLKVLSFNERAIKLYEKMGFIREGILRQALYRAGNWHDIVVMSILKNEYLSVSYK
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MFENEIIKLRKLSLNDYNIYHNWRNDVEVMYTTSPQLDTYTLEETEEFISSIISQSNAKS
CCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCC
YIIEHKKTDQAVGIVSLSNINYKDRSAECIIDIGVKDMWGKGIGTAALSLILEFAFSELN
EEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHCC
LHRISLKVLSFNERAIKLYEKMGFIREGILRQALYRAGNWHDIVVMSILKNEYLSVSYK
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA; Spermidine [C]

Specific reaction: Acetyl-CoA + Spermidine --> Coenzyme A + H+ + N8-Acetylspermidine [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2838827 [H]