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The map label for this gene is cfr [H]

Identifier: 226950274

GI number: 226950274

Start: 3256888

End: 3257922

Strand: Reverse

Name: cfr [H]

Synonym: CLM_3236

Alternate gene names: 226950274

Gene position: 3257922-3256888 (Counterclockwise)

Preceding gene: 226950275

Following gene: 226950273

Centisome position: 78.4

GC content: 32.37

Gene sequence:

>1035_bases
ATGAAACAGACAAAAACGAAATATGGAAAAATGAAACAAATAGTATCGAATTTAAAATTACCTGATTATAGATACGAGCA
GCTTATAAAAGCTATTTTTCATCAAAGAATAGATAATTTTGATGATATGCATATACTACCAAAAGCGTTAAGGATGTCTT
TAGTAAATGAGTTTAGAAAGAATGTATCTAGTGTAATACCTGTTTTTTCACAAGATTCTAAACAAGCTCAAAAATTGTTA
TTTGAATTGACTGATGGAGAAAGAATAGAAGCCGTTGGACTAAAGTATAAACAGGGGTGGGAATCGTTTTGTATTTCTTG
CCAATGCGGTTGTGGTTTTGGATGTCGTTTTTGTGCAACGGGAAGTGTTGGATTTAAACGCAATCTTACTGCTGATGAGA
TAACTGACCAATTACTTTATTTCTATTTTAATGATCATAGATTGAATAGTATTTCATTTATGGGAATGGGTGAGGCTTTT
GCAAATCCGGAGTTATTTGATGCAGTAAAAATTTTAACTGATCAAAATTTATTTGGGTTAAGTCAACGAAGAATTACTAT
TTCAACAATTGGCATTATACCAGGAATTCAAAGATTGACCCAAAAATTTCCACAAGTGAATCTGGCTTTTTCACTTCATT
CACCATTTGAAAGTCAACGAAGCGATTTAATGCCTATAAATAAAAGATTTCCATTGAATGAGGTAATGAAGACATTAGAT
GAACACATCATTCATACGGGACGACGAGTGTTTATTGCTTATATTATGCTTGAAGGAATTAATGATTCGAAAGAACATGC
AGAAGCAGTTGTAGGTTTATTGAAAAATCGTGGCTCATGGGAGCATTTATATCACATTGATTTGATACCTTATAATTCTA
CGGACAAAACAACTTTTAAATTTCAATCTTCAAGTGCTATCAAGCAATTTTGCAGTACACTAAAGAAAGCTGGTATTAGT
GCAACTGTTAGAACACAATTTGGTTCTGAAATTAGTGCTGCTTGCGGACAGTTGTGCTATGAAAATGAATTATGA

Upstream 100 bases:

>100_bases
GCAAAGCCAGATGTTTTGGGCGACGCTTGCATGGAGTAGTATAAGTCGCTAATTATACTGGCTTTGCTTCTTAACTATTA
ATAGTGAGGAGGAAGCCTAC

Downstream 100 bases:

>100_bases
TGCTAGCTCAAAAATATCCATTTGAGTAAACAAATAGTATTATTTTAAAGATCTTGAAGATTTTTAATTAAATATTAAAG
AGTATTGTAGGTCCCTCAGA

Product: chloramphenicol/florfenicol resistance protein

Products: NA

Alternate protein names: 23S rRNA m8A2503 methyltransferase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKQTKTKYGKMKQIVSNLKLPDYRYEQLIKAIFHQRIDNFDDMHILPKALRMSLVNEFRKNVSSVIPVFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISCQCGCGFGCRFCATGSVGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTQKFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAVVGLLKNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKAGIS
ATVRTQFGSEISAACGQLCYENEL

Sequences:

>Translated_344_residues
MKQTKTKYGKMKQIVSNLKLPDYRYEQLIKAIFHQRIDNFDDMHILPKALRMSLVNEFRKNVSSVIPVFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISCQCGCGFGCRFCATGSVGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTQKFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAVVGLLKNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKAGIS
ATVRTQFGSEISAACGQLCYENEL
>Mature_344_residues
MKQTKTKYGKMKQIVSNLKLPDYRYEQLIKAIFHQRIDNFDDMHILPKALRMSLVNEFRKNVSSVIPVFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISCQCGCGFGCRFCATGSVGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTQKFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAVVGLLKNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKAGIS
ATVRTQFGSEISAACGQLCYENEL

Specific function: Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family. Cfr subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=348, Percent_Identity=32.4712643678161, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR022881
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.194 [H]

Molecular weight: Translated: 39238; Mature: 39238

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS01186 EGF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTKTKYGKMKQIVSNLKLPDYRYEQLIKAIFHQRIDNFDDMHILPKALRMSLVNEFRK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NVSSVIPVFSQDSKQAQKLLFELTDGERIEAVGLKYKQGWESFCISCQCGCGFGCRFCAT
HHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHEEEEECCCCCCCCEEEC
GSVGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAFANPELFDAVKILTDQNLFGL
CCCCCCCCCCHHHHHHHHEEEEECCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCCCC
SQRRITISTIGIIPGIQRLTQKFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
CCCEEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHH
EHIIHTGRRVFIAYIMLEGINDSKEHAEAVVGLLKNRGSWEHLYHIDLIPYNSTDKTTFK
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
FQSSSAIKQFCSTLKKAGISATVRTQFGSEISAACGQLCYENEL
ECCHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTKTKYGKMKQIVSNLKLPDYRYEQLIKAIFHQRIDNFDDMHILPKALRMSLVNEFRK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NVSSVIPVFSQDSKQAQKLLFELTDGERIEAVGLKYKQGWESFCISCQCGCGFGCRFCAT
HHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHEEEEECCCCCCCCEEEC
GSVGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAFANPELFDAVKILTDQNLFGL
CCCCCCCCCCHHHHHHHHEEEEECCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCCCC
SQRRITISTIGIIPGIQRLTQKFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
CCCEEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHH
EHIIHTGRRVFIAYIMLEGINDSKEHAEAVVGLLKNRGSWEHLYHIDLIPYNSTDKTTFK
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
FQSSSAIKQFCSTLKKAGISATVRTQFGSEISAACGQLCYENEL
ECCHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA