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The map label for this gene is ligT [C]

Identifier: 226950235

GI number: 226950235

Start: 3209125

End: 3209652

Strand: Reverse

Name: ligT [C]

Synonym: CLM_3189

Alternate gene names: 226950235

Gene position: 3209652-3209125 (Counterclockwise)

Preceding gene: 226950236

Following gene: 226950233

Centisome position: 77.24

GC content: 26.89

Gene sequence:

>528_bases
ATGAGAGTATTTTATGCAGTTACATTTTATGAAGAAACAAAAGAAAAATTAATAGAGTATAAAAATATAGTTGTTAATAA
TTCAGTTAAAGGTAGATTCACAAATAAAAATAATTTTCATTTAACTTTGGAGTTTATAGGTGAAGTTGATGAAAAAAAGT
TATCTTTACTAACTAATATACTATATAAGTTACAGAATCCACCTAAGGAAGTGGTTACTTCCTATATTGGCTCATTTAAA
AGGAGAGATAAAGAGATAATTTGGCTTGGAATTGAAGAAAATAAATGCCTTATCACATTGCAAAGAGAATTAAGAAATTT
ATTAATTAATAATGGATTCCAAATTGAGGATAGAAAATATGAGCCTCATATTACAATTGGTAGAGAAATAGTAAGAAAGG
GTTTTATAGAGAAAAATATTTTTCCACCTCTACATATTCCAATAGCTTCAATAGCTCTTATGGAAGGCAAGAGAGTTAAT
AACCAGTTAGTGTATGAACCATTAGAAGAGCGAATTTTAGTAGTATAA

Upstream 100 bases:

>100_bases
ATATAAATGGAAAAGTACTTATTAAAATTGAACATGTATAATATTGGCTTAGTTTGTATAAACTAAGCCAATATTAACAA
AAAATATCTAGGTGAGAAAA

Downstream 100 bases:

>100_bases
ATAAATTATTAAAAAGCAGAGGATTATAAGAAGATATAGCTCTGCTTTTTATAATTTAACACATATAAAAATTTAGTATA
AAATTGTTATACCATATATG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MRVFYAVTFYEETKEKLIEYKNIVVNNSVKGRFTNKNNFHLTLEFIGEVDEKKLSLLTNILYKLQNPPKEVVTSYIGSFK
RRDKEIIWLGIEENKCLITLQRELRNLLINNGFQIEDRKYEPHITIGREIVRKGFIEKNIFPPLHIPIASIALMEGKRVN
NQLVYEPLEERILVV

Sequences:

>Translated_175_residues
MRVFYAVTFYEETKEKLIEYKNIVVNNSVKGRFTNKNNFHLTLEFIGEVDEKKLSLLTNILYKLQNPPKEVVTSYIGSFK
RRDKEIIWLGIEENKCLITLQRELRNLLINNGFQIEDRKYEPHITIGREIVRKGFIEKNIFPPLHIPIASIALMEGKRVN
NQLVYEPLEERILVV
>Mature_175_residues
MRVFYAVTFYEETKEKLIEYKNIVVNNSVKGRFTNKNNFHLTLEFIGEVDEKKLSLLTNILYKLQNPPKEVVTSYIGSFK
RRDKEIIWLGIEENKCLITLQRELRNLLINNGFQIEDRKYEPHITIGREIVRKGFIEKNIFPPLHIPIASIALMEGKRVN
NQLVYEPLEERILVV

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20664; Mature: 20664

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFYAVTFYEETKEKLIEYKNIVVNNSVKGRFTNKNNFHLTLEFIGEVDEKKLSLLTNI
CEEEEEEEEHHHHHHHHHHHHHEEECCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHH
LYKLQNPPKEVVTSYIGSFKRRDKEIIWLGIEENKCLITLQRELRNLLINNGFQIEDRKY
HHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCEEECCCCC
EPHITIGREIVRKGFIEKNIFPPLHIPIASIALMEGKRVNNQLVYEPLEERILVV
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEECC
>Mature Secondary Structure
MRVFYAVTFYEETKEKLIEYKNIVVNNSVKGRFTNKNNFHLTLEFIGEVDEKKLSLLTNI
CEEEEEEEEHHHHHHHHHHHHHEEECCCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHH
LYKLQNPPKEVVTSYIGSFKRRDKEIIWLGIEENKCLITLQRELRNLLINNGFQIEDRKY
HHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCEEECCCCC
EPHITIGREIVRKGFIEKNIFPPLHIPIASIALMEGKRVNNQLVYEPLEERILVV
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]