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The map label for this gene is sseA [C]

Identifier: 226950025

GI number: 226950025

Start: 2977947

End: 2978921

Strand: Direct

Name: sseA [C]

Synonym: CLM_2978

Alternate gene names: 226950025

Gene position: 2977947-2978921 (Clockwise)

Preceding gene: 226950009

Following gene: 226950027

Centisome position: 71.67

GC content: 31.28

Gene sequence:

>975_bases
ATGAAAATGAAAAAAAATTTCTTTAAAAGTTTTTCCTCTATTATTCTTTGTTTTGTTTTAGGGGTTATAATTTTTACTGG
TTGTTCTAATTCATCTTCTAATAAAGAAGAAACAAAGGATAAAAAGCAAGAAACAACACAAAAAGAATCCTATAAAGACA
GTAGTTACATAGTAGGTTCTGACTGGCTATCTAAAAATTTAAATAAAGATAATGTAATAATAGTAGATGCTCGCCCTGAC
AAAGACTATAAAAAAGGTCATATACCTGGAGCTATAAATGTTCAGTGGCCATATTTTACTAACCAGGAAGGGAAACCTGG
TGAAAAGGATTGGGGAATGCTTTTACCAGAAAAAGAACTTTCTAAAAAATTGTCCTCCCTAGGTATAGATAAAAATAAAA
CCATCGTTGCTTATGCAGAAAATAAAAGTGGTTGGGGAGAAGATGGAAGAATAATTTGGATGCTTAGAATGGTAGGCATA
GAAAATTCCAAAATGTTAAATGGTGGATTTGACTATTGGAAAAACAAAAGCTTAGAAATATCAAAAGATGATGTTACTCC
TAAAAAAAGTGATTTTGTAGTAGAGAACATGGACAAATCTATGTATGCAGATACAAAGTGGGTTAAAGAAAACTTAGACA
AAATAAAAATATTAGATGCTAGAGAAGAAGTAGAATATAAGGGTGCAACAAAATATGGTGAGGCTAGAGGTGGACACCTA
CCTGGAGCTAAGCTATTTACTTTTAATAAAGCATTCAATGAGGATCAAAGTTTAAAAAGCCAAAAAGAAATAGAAGGCCT
GTTAACAGAAGCTGGTATTAAAAAAGATGATGAAATACTTACTTACTGCACTGCTGGTATAAGATCTGCCCACTTAGCTT
TAGTATTAAAAACGTGCGGATATAATAATGTTAAAAACTATGATGGTTCCTTCTATATGTGGTCAGCAGATAAGAATTTA
CAGATAGAAAAATAA

Upstream 100 bases:

>100_bases
GGTTATAGTCCACCTATTTGTATTTTTTATTAACTTTTATATGTAAAAAATTAAATGATATGCTAATTGCAACATCTGTT
AAAAATGAGTGGAATAATAT

Downstream 100 bases:

>100_bases
TTTGAATATATTAATTAAATATCACATGCATTGTATAATTTTAAAATAGAACTATATTGAAGCATTAGTTGGTTAAATTT
AAAATATTGTATAGTAATCT

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKMKKNFFKSFSSIILCFVLGVIIFTGCSNSSSNKEETKDKKQETTQKESYKDSSYIVGSDWLSKNLNKDNVIIVDARPD
KDYKKGHIPGAINVQWPYFTNQEGKPGEKDWGMLLPEKELSKKLSSLGIDKNKTIVAYAENKSGWGEDGRIIWMLRMVGI
ENSKMLNGGFDYWKNKSLEISKDDVTPKKSDFVVENMDKSMYADTKWVKENLDKIKILDAREEVEYKGATKYGEARGGHL
PGAKLFTFNKAFNEDQSLKSQKEIEGLLTEAGIKKDDEILTYCTAGIRSAHLALVLKTCGYNNVKNYDGSFYMWSADKNL
QIEK

Sequences:

>Translated_324_residues
MKMKKNFFKSFSSIILCFVLGVIIFTGCSNSSSNKEETKDKKQETTQKESYKDSSYIVGSDWLSKNLNKDNVIIVDARPD
KDYKKGHIPGAINVQWPYFTNQEGKPGEKDWGMLLPEKELSKKLSSLGIDKNKTIVAYAENKSGWGEDGRIIWMLRMVGI
ENSKMLNGGFDYWKNKSLEISKDDVTPKKSDFVVENMDKSMYADTKWVKENLDKIKILDAREEVEYKGATKYGEARGGHL
PGAKLFTFNKAFNEDQSLKSQKEIEGLLTEAGIKKDDEILTYCTAGIRSAHLALVLKTCGYNNVKNYDGSFYMWSADKNL
QIEK
>Mature_324_residues
MKMKKNFFKSFSSIILCFVLGVIIFTGCSNSSSNKEETKDKKQETTQKESYKDSSYIVGSDWLSKNLNKDNVIIVDARPD
KDYKKGHIPGAINVQWPYFTNQEGKPGEKDWGMLLPEKELSKKLSSLGIDKNKTIVAYAENKSGWGEDGRIIWMLRMVGI
ENSKMLNGGFDYWKNKSLEISKDDVTPKKSDFVVENMDKSMYADTKWVKENLDKIKILDAREEVEYKGATKYGEARGGHL
PGAKLFTFNKAFNEDQSLKSQKEIEGLLTEAGIKKDDEILTYCTAGIRSAHLALVLKTCGYNNVKNYDGSFYMWSADKNL
QIEK

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=276, Percent_Identity=25.3623188405797, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI194473668, Length=308, Percent_Identity=23.0519480519481, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI61835204, Length=246, Percent_Identity=26.0162601626016, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI194473681, Length=246, Percent_Identity=26.0162601626016, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87082121, Length=285, Percent_Identity=25.9649122807018, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87081967, Length=254, Percent_Identity=24.0157480314961, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71997283, Length=291, Percent_Identity=26.8041237113402, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17561888, Length=309, Percent_Identity=24.5954692556634, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=258, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 36816; Mature: 36816

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKKNFFKSFSSIILCFVLGVIIFTGCSNSSSNKEETKDKKQETTQKESYKDSSYIVGS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEC
DWLSKNLNKDNVIIVDARPDKDYKKGHIPGAINVQWPYFTNQEGKPGEKDWGMLLPEKEL
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCCCCHHHH
SKKLSSLGIDKNKTIVAYAENKSGWGEDGRIIWMLRMVGIENSKMLNGGFDYWKNKSLEI
HHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEECCCHHHHCCCCEEE
SKDDVTPKKSDFVVENMDKSMYADTKWVKENLDKIKILDAREEVEYKGATKYGEARGGHL
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCCCC
PGAKLFTFNKAFNEDQSLKSQKEIEGLLTEAGIKKDDEILTYCTAGIRSAHLALVLKTCG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
YNNVKNYDGSFYMWSADKNLQIEK
CCCCCCCCCCEEEEECCCCCEECC
>Mature Secondary Structure
MKMKKNFFKSFSSIILCFVLGVIIFTGCSNSSSNKEETKDKKQETTQKESYKDSSYIVGS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEC
DWLSKNLNKDNVIIVDARPDKDYKKGHIPGAINVQWPYFTNQEGKPGEKDWGMLLPEKEL
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCCCCHHHH
SKKLSSLGIDKNKTIVAYAENKSGWGEDGRIIWMLRMVGIENSKMLNGGFDYWKNKSLEI
HHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEECCCHHHHCCCCEEE
SKDDVTPKKSDFVVENMDKSMYADTKWVKENLDKIKILDAREEVEYKGATKYGEARGGHL
ECCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCCCCC
PGAKLFTFNKAFNEDQSLKSQKEIEGLLTEAGIKKDDEILTYCTAGIRSAHLALVLKTCG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
YNNVKNYDGSFYMWSADKNLQIEK
CCCCCCCCCCEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]