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The map label for this gene is yclF [H]

Identifier: 226949442

GI number: 226949442

Start: 2447529

End: 2448935

Strand: Direct

Name: yclF [H]

Synonym: CLM_2372

Alternate gene names: 226949442

Gene position: 2447529-2448935 (Clockwise)

Preceding gene: 226949412

Following gene: 226949445

Centisome position: 58.9

GC content: 33.69

Gene sequence:

>1407_bases
ATGTCTGAAAAAAAACAAAAACATCCTATTGGATTATGGCTAGTAAATATCTCCATAGCTTTACAAAGTTATGCAGCTTA
TGCAGTAGTTTCAATATTAATACTATTTTTAACAGCTGACGTTAGCAAAAATGGACTTGGTCTAAGTGTACCAAAAGCCG
CTGCTATCATAGGTTTATACCAAGGTATAAACTATATGGGTTCCCTAGTTGGTGGATATATAACTGATAAATGGCTTGGA
ATCCAGAAATCACTAATATTAGGTTGTTTTCTAACTGCTTGTGGTTATTTGGCGCTATTTTTCGCCAAACCAAATATTGG
TAGTGTCTGGTTGGGACTTATTATCCTAATTGTTGCTGGTGCATTTTTCAAGGCACAAATTAGTAGCCTTGTAGGTGAAC
TTTATGGCAAAAATGAATTGTCTAAAAAAGATGCTGCCTATAGCATATTTTATATGTTTATAAATATCGGTGCTTTTTTT
GGACCTATAATTGCTGGTTTAATTTCTGATAAATGGTTCGCTAAAGTAGCCGCTGATGGTGAAATCGCAGCCTATGGTTA
TAAATACATTTTTCTTATGTGTACTTTGATAATGATATTTATAGGTATCTTTATTTGGATTGTATCCCCTAAATGGCTAG
GTGAGATAGGAAAATACCCAGCTTCCAAAGATAAAAAAACTTCCACAGAATCCGTAAGCACAAAATCAACAGAGAAAGCT
AAAGCACAACCTTTGACACAATTAGAAAAAAATCGTATTAAAGCAATGATTGTGATGTTTGTGTTTGTCATTATATTTTG
GTCAGCATGGGATCAAACTGCTACATCCTTCTCTTTACTTGCAAATAAACTTGTAAACAGAACCATCGGTGGATTTACAA
TGCCTGTACCTTGGCTTACTTCTATTAATGCAATCTTTTGCGTAGTATTGTCACCAATAATTGCGAATATATGGTTAAAA
CTTGGGAATAGTAAAAAGGGCGACTTATCAGTTCCTACTAAAATGGGACTTGGAATGATTTTAACAGGTATTGCCTTCTT
AACCTTAATACTAGGTATATCAACATTAGGTGGTGTTTTAGACGGAAGTAAACAAATGAACATACTTTTCATCATTGTGG
CTTACTTTTTATTAACAATAGGAGAGCTTTGCCTATCTCCAATAGGAATGGCAATGTTCAATAAATTAGCTCCTGCAAAA
TACGGCTCCCTAGCAATGGGTGCATGGTACTTAAGCTTCTTCTTTTCAAGTATAATTTCAGGAAAACTTGCAGGATTTAC
AGATACCCTAGGATATACCCAAATATTTGGCTTAATATCTGGCATTGTAATAGTATTTGGTGTAATTCTAATTTTATTAC
GAAATGGATTATTAAAACTAATGTCTCTAGACGAATTAGATAAATAA

Upstream 100 bases:

>100_bases
GTATGCAAAGGTGAAAATAATGGTTAAAAGAATACACTAGAGAAATTTAATAATTGTAAAAATTTAATAATTATAAATAA
TAGAAGGAAGGTATTTCACA

Downstream 100 bases:

>100_bases
TAACATAAAACAGTATTACAAAACAGAAACCTATCTTAAGCCTTATATTCCAATAGCTAAGATAGGTTTTAAAATTAAAA
TTGGTTAGAAAAATATTTTA

Product: amino acid/peptide transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSEKKQKHPIGLWLVNISIALQSYAAYAVVSILILFLTADVSKNGLGLSVPKAAAIIGLYQGINYMGSLVGGYITDKWLG
IQKSLILGCFLTACGYLALFFAKPNIGSVWLGLIILIVAGAFFKAQISSLVGELYGKNELSKKDAAYSIFYMFINIGAFF
GPIIAGLISDKWFAKVAADGEIAAYGYKYIFLMCTLIMIFIGIFIWIVSPKWLGEIGKYPASKDKKTSTESVSTKSTEKA
KAQPLTQLEKNRIKAMIVMFVFVIIFWSAWDQTATSFSLLANKLVNRTIGGFTMPVPWLTSINAIFCVVLSPIIANIWLK
LGNSKKGDLSVPTKMGLGMILTGIAFLTLILGISTLGGVLDGSKQMNILFIIVAYFLLTIGELCLSPIGMAMFNKLAPAK
YGSLAMGAWYLSFFFSSIISGKLAGFTDTLGYTQIFGLISGIVIVFGVILILLRNGLLKLMSLDELDK

Sequences:

>Translated_468_residues
MSEKKQKHPIGLWLVNISIALQSYAAYAVVSILILFLTADVSKNGLGLSVPKAAAIIGLYQGINYMGSLVGGYITDKWLG
IQKSLILGCFLTACGYLALFFAKPNIGSVWLGLIILIVAGAFFKAQISSLVGELYGKNELSKKDAAYSIFYMFINIGAFF
GPIIAGLISDKWFAKVAADGEIAAYGYKYIFLMCTLIMIFIGIFIWIVSPKWLGEIGKYPASKDKKTSTESVSTKSTEKA
KAQPLTQLEKNRIKAMIVMFVFVIIFWSAWDQTATSFSLLANKLVNRTIGGFTMPVPWLTSINAIFCVVLSPIIANIWLK
LGNSKKGDLSVPTKMGLGMILTGIAFLTLILGISTLGGVLDGSKQMNILFIIVAYFLLTIGELCLSPIGMAMFNKLAPAK
YGSLAMGAWYLSFFFSSIISGKLAGFTDTLGYTQIFGLISGIVIVFGVILILLRNGLLKLMSLDELDK
>Mature_467_residues
SEKKQKHPIGLWLVNISIALQSYAAYAVVSILILFLTADVSKNGLGLSVPKAAAIIGLYQGINYMGSLVGGYITDKWLGI
QKSLILGCFLTACGYLALFFAKPNIGSVWLGLIILIVAGAFFKAQISSLVGELYGKNELSKKDAAYSIFYMFINIGAFFG
PIIAGLISDKWFAKVAADGEIAAYGYKYIFLMCTLIMIFIGIFIWIVSPKWLGEIGKYPASKDKKTSTESVSTKSTEKAK
AQPLTQLEKNRIKAMIVMFVFVIIFWSAWDQTATSFSLLANKLVNRTIGGFTMPVPWLTSINAIFCVVLSPIIANIWLKL
GNSKKGDLSVPTKMGLGMILTGIAFLTLILGISTLGGVLDGSKQMNILFIIVAYFLLTIGELCLSPIGMAMFNKLAPAKY
GSLAMGAWYLSFFFSSIISGKLAGFTDTLGYTQIFGLISGIVIVFGVILILLRNGLLKLMSLDELDK

Specific function: Unknown

COG id: COG3104

COG function: function code E; Dipeptide/tripeptide permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTR2/POT transporter (TC 2.A.17) family [H]

Homologues:

Organism=Escherichia coli, GI1787922, Length=474, Percent_Identity=25.5274261603376, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789911, Length=490, Percent_Identity=25.1020408163265, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790572, Length=414, Percent_Identity=25.6038647342995, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786927, Length=173, Percent_Identity=27.1676300578035, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR000109
- InterPro:   IPR005279
- InterPro:   IPR018456 [H]

Pfam domain/function: PF00854 PTR2 [H]

EC number: NA

Molecular weight: Translated: 50859; Mature: 50727

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50850 MFS ; PS01023 PTR2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKQKHPIGLWLVNISIALQSYAAYAVVSILILFLTADVSKNGLGLSVPKAAAIIGLY
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
QGINYMGSLVGGYITDKWLGIQKSLILGCFLTACGYLALFFAKPNIGSVWLGLIILIVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AFFKAQISSLVGELYGKNELSKKDAAYSIFYMFINIGAFFGPIIAGLISDKWFAKVAADG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
EIAAYGYKYIFLMCTLIMIFIGIFIWIVSPKWLGEIGKYPASKDKKTSTESVSTKSTEKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHCCCCCHHHH
KAQPLTQLEKNRIKAMIVMFVFVIIFWSAWDQTATSFSLLANKLVNRTIGGFTMPVPWLT
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SINAIFCVVLSPIIANIWLKLGNSKKGDLSVPTKMGLGMILTGIAFLTLILGISTLGGVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGSKQMNILFIIVAYFLLTIGELCLSPIGMAMFNKLAPAKYGSLAMGAWYLSFFFSSIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GKLAGFTDTLGYTQIFGLISGIVIVFGVILILLRNGLLKLMSLDELDK
CHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEKKQKHPIGLWLVNISIALQSYAAYAVVSILILFLTADVSKNGLGLSVPKAAAIIGLY
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH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CHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]