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The map label for this gene is kanC [H]

Identifier: 226949429

GI number: 226949429

Start: 2432595

End: 2433734

Strand: Reverse

Name: kanC [H]

Synonym: CLM_2357

Alternate gene names: 226949429

Gene position: 2433734-2432595 (Counterclockwise)

Preceding gene: 226949430

Following gene: 226949428

Centisome position: 58.57

GC content: 27.19

Gene sequence:

>1140_bases
ATGTATATGAATTTAGCACAAAAAATTAATGATGATTATTATCATCCAGAGGTTAAACTTGAAACAAACTTATTAGAATC
ATCTCCAATATATTTAGGGTGCAATATATGGAGAGAAAGTCTATTAGAAAAGATGATGGATTTAAATACAGATAAGTTTT
TCTTAATTACAGACGATGTTGTATATAATTTGTTTGGAAAAGAACTATTGGAATATATGAATAGAAAAGTTTCAGTAAAA
TTAATTAAATTGCCATCAGGTGAAAAACATAAAAATATAAAAGTGTTTAATGATTTAATGGAAGATTTATTTGATAACAA
TGTAACTAAAAGTTCAATCTTAATTTCATTAGGTGGAGGTGTTGTAGGGAATATTACAGGATTGGCTGCTGCTTTAGCAT
TTAGAGGTATTAGATTTTTTCATATACCTACAACTTTCATGTCTCAGACTGATAGCATTCTTAGCAGAAAACAAGGGATT
AATTCTTTTTATGGTAAGAATATGATTGGATCATATTATACACCTTTATTTAATTTTATAGATACTTCATTTTTAATATT
TGATAGTGAAAGATTCATAAGAGGTAGTTTTGTTGAAACTGTTAAAAATGGATTCATATATAATGCAGATTTTTTAAATA
AACTTAAATCAGTAATTAAAAATGATTTTAATGTTAACCAAGAAGGCATTTTTAATTTAGTAAAAATGAGTATTGAATCA
AAGTTACCAATAATGAAAGCAGATCCAACTGAAAAAGGTTTAGCTATGATTCTAGAATATGGTCATACCGTTGGACATGC
AATAGAAAAGCTTTCATATGGAAAACTATCACATGGAGAAAGTGTATCAATTGGTATGATGGTTGCTGCTCGCGTAAGTG
AAAAACTTGGTTACTTAAGTAAGCAGGATGTTAAGGAGCATCTGGATATATTATCAGCATTGAAGACACCTACAAAAATT
CCTTCAAATATAAAAATTTCTGACATAATTAATAGAATAAAATTAGATAATAAAAAAGACATGAATGGTATAAGGTTTGT
TGTTCTTGAAAGCATAGGAAAATGCATAAACACTGATGGTAGTTATATGATTAAGGTTCCTTTTAATATAATCAATGAGG
CTATAGAAGAAACTTGTTAA

Upstream 100 bases:

>100_bases
GAAGGAGTGATGTTTTAATTTAAAATATTAATAGATTGCTTTGTTTATCAGAGTAGTTTTGTTAAAAAACATATTATATA
AATAAACAGGAGGAATGAAA

Downstream 100 bases:

>100_bases
TTTTAGTGAATTTATTATAAAAATATACTAGATTTATAAGTTTAAAATAGGATATTTGATTTCAAAATTTAAAGGAGGAG
AATATGTTAAAACTTGCTTA

Product: putative 3-dehydroquinate synthase

Products: dehydroquinate +phosphate [C]

Alternate protein names: DOI synthase; DOIS [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MYMNLAQKINDDYYHPEVKLETNLLESSPIYLGCNIWRESLLEKMMDLNTDKFFLITDDVVYNLFGKELLEYMNRKVSVK
LIKLPSGEKHKNIKVFNDLMEDLFDNNVTKSSILISLGGGVVGNITGLAAALAFRGIRFFHIPTTFMSQTDSILSRKQGI
NSFYGKNMIGSYYTPLFNFIDTSFLIFDSERFIRGSFVETVKNGFIYNADFLNKLKSVIKNDFNVNQEGIFNLVKMSIES
KLPIMKADPTEKGLAMILEYGHTVGHAIEKLSYGKLSHGESVSIGMMVAARVSEKLGYLSKQDVKEHLDILSALKTPTKI
PSNIKISDIINRIKLDNKKDMNGIRFVVLESIGKCINTDGSYMIKVPFNIINEAIEETC

Sequences:

>Translated_379_residues
MYMNLAQKINDDYYHPEVKLETNLLESSPIYLGCNIWRESLLEKMMDLNTDKFFLITDDVVYNLFGKELLEYMNRKVSVK
LIKLPSGEKHKNIKVFNDLMEDLFDNNVTKSSILISLGGGVVGNITGLAAALAFRGIRFFHIPTTFMSQTDSILSRKQGI
NSFYGKNMIGSYYTPLFNFIDTSFLIFDSERFIRGSFVETVKNGFIYNADFLNKLKSVIKNDFNVNQEGIFNLVKMSIES
KLPIMKADPTEKGLAMILEYGHTVGHAIEKLSYGKLSHGESVSIGMMVAARVSEKLGYLSKQDVKEHLDILSALKTPTKI
PSNIKISDIINRIKLDNKKDMNGIRFVVLESIGKCINTDGSYMIKVPFNIINEAIEETC
>Mature_379_residues
MYMNLAQKINDDYYHPEVKLETNLLESSPIYLGCNIWRESLLEKMMDLNTDKFFLITDDVVYNLFGKELLEYMNRKVSVK
LIKLPSGEKHKNIKVFNDLMEDLFDNNVTKSSILISLGGGVVGNITGLAAALAFRGIRFFHIPTTFMSQTDSILSRKQGI
NSFYGKNMIGSYYTPLFNFIDTSFLIFDSERFIRGSFVETVKNGFIYNADFLNKLKSVIKNDFNVNQEGIFNLVKMSIES
KLPIMKADPTEKGLAMILEYGHTVGHAIEKLSYGKLSHGESVSIGMMVAARVSEKLGYLSKQDVKEHLDILSALKTPTKI
PSNIKISDIINRIKLDNKKDMNGIRFVVLESIGKCINTDGSYMIKVPFNIINEAIEETC

Specific function: Catalyzes the intramolecular carbocycle formation from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose (DOI) [H]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family. DOI synthase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789791, Length=306, Percent_Identity=31.6993464052288, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320332, Length=319, Percent_Identity=29.4670846394984, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016303
- InterPro:   IPR002658 [H]

Pfam domain/function: PF01761 DHQ_synthase [H]

EC number: 4.2.3.4 [C]

Molecular weight: Translated: 42895; Mature: 42895

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMNLAQKINDDYYHPEVKLETNLLESSPIYLGCNIWRESLLEKMMDLNTDKFFLITDDV
CCCCHHHHCCCCCCCCCEEEEECHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHH
VYNLFGKELLEYMNRKVSVKLIKLPSGEKHKNIKVFNDLMEDLFDNNVTKSSILISLGGG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC
VVGNITGLAAALAFRGIRFFHIPTTFMSQTDSILSRKQGINSFYGKNMIGSYYTPLFNFI
CHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHH
DTSFLIFDSERFIRGSFVETVKNGFIYNADFLNKLKSVIKNDFNVNQEGIFNLVKMSIES
CCEEEEECCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLPIMKADPTEKGLAMILEYGHTVGHAIEKLSYGKLSHGESVSIGMMVAARVSEKLGYLS
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
KQDVKEHLDILSALKTPTKIPSNIKISDIINRIKLDNKKDMNGIRFVVLESIGKCINTDG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
SYMIKVPFNIINEAIEETC
CEEEEECHHHHHHHHHHCC
>Mature Secondary Structure
MYMNLAQKINDDYYHPEVKLETNLLESSPIYLGCNIWRESLLEKMMDLNTDKFFLITDDV
CCCCHHHHCCCCCCCCCEEEEECHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHH
VYNLFGKELLEYMNRKVSVKLIKLPSGEKHKNIKVFNDLMEDLFDNNVTKSSILISLGGG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC
VVGNITGLAAALAFRGIRFFHIPTTFMSQTDSILSRKQGINSFYGKNMIGSYYTPLFNFI
CHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHH
DTSFLIFDSERFIRGSFVETVKNGFIYNADFLNKLKSVIKNDFNVNQEGIFNLVKMSIES
CCEEEEECCCHHHHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KLPIMKADPTEKGLAMILEYGHTVGHAIEKLSYGKLSHGESVSIGMMVAARVSEKLGYLS
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
KQDVKEHLDILSALKTPTKIPSNIKISDIINRIKLDNKKDMNGIRFVVLESIGKCINTDG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
SYMIKVPFNIINEAIEETC
CEEEEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Co2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.033 {3-deoxy-D-arabino-heptulosonic} [C]

Substrates: @DEOX04.txt*phospho-deoxy-arabino-heptulosonate! [C]

Specific reaction: @DEOX04.txt*phospho-deoxy-arabino-heptulosonate! = dehydroquinate +phosphate [C]

General reaction: elimination (P-O bond cleavage [C]

Inhibitor: 3-deoxy-D-arabino-heoptulosonic acid; 3-deoxy-D-arabino-heptulosonic acid7-homophosphate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA