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The map label for this gene is tobE [H]

Identifier: 226949427

GI number: 226949427

Start: 2430547

End: 2431587

Strand: Reverse

Name: tobE [H]

Synonym: CLM_2355

Alternate gene names: 226949427

Gene position: 2431587-2430547 (Counterclockwise)

Preceding gene: 226949428

Following gene: 226949426

Centisome position: 58.52

GC content: 26.71

Gene sequence:

>1041_bases
GTGGAAGAAGGAGTTATTATGAAGGGGTTGAAATTAATTTCACCCAAGGTAATAAAAAAAATAGATTTAGATATACCTCA
AAACATACCTAAAGAACATGCTTTAATAAAAATAAATACTATAAGTCTTTGTGGATCAGATTATAAATTATTTTATGGTG
ATTATAGTGGCCCGTGTAGGTATCCTTTATATTTTGGTCATGAGTGGTCTGGAGAAGTTGTTGAAATAAATGATGATACA
TCAAACATTAAAATTGGAGATAAGGTAACAGGAGATTGTTCCAAATGGTGCGGCAATTGTTGTTTATGCAAAGAAGATAA
AAATTTATGTAGCAACATTGAAAAGTTTGGAATAACTATTGATGGATATTCAAGACAATATGTAGTTGTAGAAACAAAAT
ATTTATATGTAGCACCAATGAAAACATCTTTTGATATTCTTGCTCTAACAGAATGTTTTTCAGTAGCACTGCATGCTATA
AAAAAGATAGAATCAATGTTATATAAAAAGGATAGATGCATATTAATAATGGGTGGAGGAGCAATAGGATTATCAATTTA
TTTATTATTAAAATTTAAGTTTAACTATTATAATATTAAATTTATGGAAATTGATGATAGTAAAAAAGATTTTATTAGTA
AGGTTTTTGATGTGAATTTTGCAAGTGAAAAAAGTTTTGAAGTAAATAATAGTTCATACAGTGAAATTTCAAAACATTGT
AACTATGATATTATTTTTGAAGCAAGTGGAAATATAGAAGCATTTCAGCAAGCTTTATATTTAATTAATCCTAATGGACA
TATCATAACACTAGGAATGTATCCTAATAAAAATGTAACTTTATCACCAATTACTTTGAAATCAATTAAAGTTCATGGAA
CAATTGGAGGAACACATGAATTTGATGAGGTTATTAAGTTTTTAATTTCAAATAAAGATGAAGTTAAAAAGATGATAACA
CATACCTATGGATTGGATGATGCTAGTATGGGATTTCAAGTTAAAGATAAGAGAATTAAAACTCAAATAAATTTATGGTG
A

Upstream 100 bases:

>100_bases
AAGAGAATTTCCTAGTATAAAATTAGTACAATAAAGTTAAATGATTATGAATGAAACTTTATAAAATCTATAATAGAGTA
ATCATTAATTTTATAAAAAG

Downstream 100 bases:

>100_bases
TATAAAATATATTTTATATTAAATTGTATATTTGCAGACATACAGGTAGTCTAAGTTTGAATTGGACTACCTGTTTTTGA
TATATAAATTTATTATTAGT

Product: zinc-binding dehydrogenase family oxidoreductase

Products: L-sorbose; NADH; H+

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MEEGVIMKGLKLISPKVIKKIDLDIPQNIPKEHALIKINTISLCGSDYKLFYGDYSGPCRYPLYFGHEWSGEVVEINDDT
SNIKIGDKVTGDCSKWCGNCCLCKEDKNLCSNIEKFGITIDGYSRQYVVVETKYLYVAPMKTSFDILALTECFSVALHAI
KKIESMLYKKDRCILIMGGGAIGLSIYLLLKFKFNYYNIKFMEIDDSKKDFISKVFDVNFASEKSFEVNNSSYSEISKHC
NYDIIFEASGNIEAFQQALYLINPNGHIITLGMYPNKNVTLSPITLKSIKVHGTIGGTHEFDEVIKFLISNKDEVKKMIT
HTYGLDDASMGFQVKDKRIKTQINLW

Sequences:

>Translated_346_residues
MEEGVIMKGLKLISPKVIKKIDLDIPQNIPKEHALIKINTISLCGSDYKLFYGDYSGPCRYPLYFGHEWSGEVVEINDDT
SNIKIGDKVTGDCSKWCGNCCLCKEDKNLCSNIEKFGITIDGYSRQYVVVETKYLYVAPMKTSFDILALTECFSVALHAI
KKIESMLYKKDRCILIMGGGAIGLSIYLLLKFKFNYYNIKFMEIDDSKKDFISKVFDVNFASEKSFEVNNSSYSEISKHC
NYDIIFEASGNIEAFQQALYLINPNGHIITLGMYPNKNVTLSPITLKSIKVHGTIGGTHEFDEVIKFLISNKDEVKKMIT
HTYGLDDASMGFQVKDKRIKTQINLW
>Mature_346_residues
MEEGVIMKGLKLISPKVIKKIDLDIPQNIPKEHALIKINTISLCGSDYKLFYGDYSGPCRYPLYFGHEWSGEVVEINDDT
SNIKIGDKVTGDCSKWCGNCCLCKEDKNLCSNIEKFGITIDGYSRQYVVVETKYLYVAPMKTSFDILALTECFSVALHAI
KKIESMLYKKDRCILIMGGGAIGLSIYLLLKFKFNYYNIKFMEIDDSKKDFISKVFDVNFASEKSFEVNNSSYSEISKHC
NYDIIFEASGNIEAFQQALYLINPNGHIITLGMYPNKNVTLSPITLKSIKVHGTIGGTHEFDEVIKFLISNKDEVKKMIT
HTYGLDDASMGFQVKDKRIKTQINLW

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=345, Percent_Identity=23.1884057971014, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1790045, Length=333, Percent_Identity=27.3273273273273, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787863, Length=323, Percent_Identity=24.7678018575851, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788073, Length=341, Percent_Identity=26.099706744868, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788075, Length=329, Percent_Identity=25.531914893617, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI226510992, Length=310, Percent_Identity=22.9032258064516, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790718, Length=323, Percent_Identity=24.4582043343653, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788407, Length=318, Percent_Identity=21.3836477987421, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562876, Length=323, Percent_Identity=22.6006191950464, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562878, Length=206, Percent_Identity=27.6699029126214, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=360, Percent_Identity=26.9444444444444, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=326, Percent_Identity=26.0736196319018, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=326, Percent_Identity=26.0736196319018, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=355, Percent_Identity=25.6338028169014, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323099, Length=345, Percent_Identity=25.2173913043478, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=302, Percent_Identity=22.5165562913907, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319621, Length=333, Percent_Identity=21.021021021021, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17737897, Length=328, Percent_Identity=23.1707317073171, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137530, Length=332, Percent_Identity=22.289156626506, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.14

Molecular weight: Translated: 39261; Mature: 39261

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEGVIMKGLKLISPKVIKKIDLDIPQNIPKEHALIKINTISLCGSDYKLFYGDYSGPCR
CCCCEEECCHHHCCHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCC
YPLYFGHEWSGEVVEINDDTSNIKIGDKVTGDCSKWCGNCCLCKEDKNLCSNIEKFGITI
CCEEECCCCCCCEEEECCCCCCEEECCCCCCCHHHHCCCEEEECCCHHHHCCHHHHCEEE
DGYSRQYVVVETKYLYVAPMKTSFDILALTECFSVALHAIKKIESMLYKKDRCILIMGGG
ECCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AIGLSIYLLLKFKFNYYNIKFMEIDDSKKDFISKVFDVNFASEKSFEVNNSSYSEISKHC
HHHEEEEEEEEEEECEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHC
NYDIIFEASGNIEAFQQALYLINPNGHIITLGMYPNKNVTLSPITLKSIKVHGTIGGTHE
CEEEEEEECCCHHHHEEEEEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEEEECCCCCC
FDEVIKFLISNKDEVKKMITHTYGLDDASMGFQVKDKRIKTQINLW
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEECC
>Mature Secondary Structure
MEEGVIMKGLKLISPKVIKKIDLDIPQNIPKEHALIKINTISLCGSDYKLFYGDYSGPCR
CCCCEEECCHHHCCHHHHHEECCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCC
YPLYFGHEWSGEVVEINDDTSNIKIGDKVTGDCSKWCGNCCLCKEDKNLCSNIEKFGITI
CCEEECCCCCCCEEEECCCCCCEEECCCCCCCHHHHCCCEEEECCCHHHHCCHHHHCEEE
DGYSRQYVVVETKYLYVAPMKTSFDILALTECFSVALHAIKKIESMLYKKDRCILIMGGG
ECCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
AIGLSIYLLLKFKFNYYNIKFMEIDDSKKDFISKVFDVNFASEKSFEVNNSSYSEISKHC
HHHEEEEEEEEEEECEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHC
NYDIIFEASGNIEAFQQALYLINPNGHIITLGMYPNKNVTLSPITLKSIKVHGTIGGTHE
CEEEEEEECCCHHHHEEEEEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEEEECCCCCC
FDEVIKFLISNKDEVKKMITHTYGLDDASMGFQVKDKRIKTQINLW
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-iditol; NAD+

Specific reaction: L-iditol + NAD+ = L-sorbose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA