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The map label for this gene is graR [H]

Identifier: 226949419

GI number: 226949419

Start: 2421230

End: 2421901

Strand: Reverse

Name: graR [H]

Synonym: CLM_2347

Alternate gene names: 226949419

Gene position: 2421901-2421230 (Counterclockwise)

Preceding gene: 226949420

Following gene: 226949418

Centisome position: 58.28

GC content: 27.83

Gene sequence:

>672_bases
ATGAAAAAAATCCTTATTATTGAAGATGATGAAGTTATAAGAGAAGAATTACAGAATTTTTTAAGAAAATATGGATACGA
AGTAAATGCACCTATAGAGTTTAATAATATAATACAATATGTTGAAAAGGAGAACGCTAGCCTTATATTACTAGACATAA
ACCTTCCAGAGTTTGATGGTTACTATATATGTAGAGAAATACGTAAAACATCAGATGTTCCAATTATAATAGTTACAAGT
AGAGATAGTGAAGTAGATGAATTGATTAGTATGAATTTAGGAGCAGATGATTTTGTAACAAAGCCTTACAATACAGAAAT
ATTATTAGCAAGAATAACAAATATATTAAAAAGAACCTATGGAAACTTAAAAACTAATAACACATTAAATTACAAGGATT
TTAATTTAAATCTTTCAAATGCAACGCTTATTTATAAAGATAAATCTTTGGAGTTGACGAAAAATGAGGTTAAGATACTT
TCCTATTTGATAAACAATAGGGGCAATATAGTGAAAAGAGATTCACTTATGGAGTACTTGTGGAAATCAGATTATTTTGT
GGATGATAGCACCTTAACTGTTAATATTAATAGATTAAGAAAGAAGCTTCAAGAGATAGGGATAGAAAATCCAATAGAAA
CAAGGAGAGGGTTAGGGTATATAATGCCATGA

Upstream 100 bases:

>100_bases
AAAGGTAGTATTTAATCTTATAATGATTATTAGGGCTATTAGATTTTGTTTCCCAAAGTTTAATAGCCTTATATTCTATG
CTAGGGAGGTAGATTAAATA

Downstream 100 bases:

>100_bases
GTATAGGTGAATTTATTAAGGATAAAATTCTTATAATATCATGTAATATGCTGATTTTTATTTTATTATCAATTATAATA
CTTGCTAGTAATGTTGATTT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein R [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKKILIIEDDEVIREELQNFLRKYGYEVNAPIEFNNIIQYVEKENASLILLDINLPEFDGYYICREIRKTSDVPIIIVTS
RDSEVDELISMNLGADDFVTKPYNTEILLARITNILKRTYGNLKTNNTLNYKDFNLNLSNATLIYKDKSLELTKNEVKIL
SYLINNRGNIVKRDSLMEYLWKSDYFVDDSTLTVNINRLRKKLQEIGIENPIETRRGLGYIMP

Sequences:

>Translated_223_residues
MKKILIIEDDEVIREELQNFLRKYGYEVNAPIEFNNIIQYVEKENASLILLDINLPEFDGYYICREIRKTSDVPIIIVTS
RDSEVDELISMNLGADDFVTKPYNTEILLARITNILKRTYGNLKTNNTLNYKDFNLNLSNATLIYKDKSLELTKNEVKIL
SYLINNRGNIVKRDSLMEYLWKSDYFVDDSTLTVNINRLRKKLQEIGIENPIETRRGLGYIMP
>Mature_223_residues
MKKILIIEDDEVIREELQNFLRKYGYEVNAPIEFNNIIQYVEKENASLILLDINLPEFDGYYICREIRKTSDVPIIIVTS
RDSEVDELISMNLGADDFVTKPYNTEILLARITNILKRTYGNLKTNNTLNYKDFNLNLSNATLIYKDKSLELTKNEVKIL
SYLINNRGNIVKRDSLMEYLWKSDYFVDDSTLTVNINRLRKKLQEIGIENPIETRRGLGYIMP

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs) [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=32.5991189427313, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=33.1838565022422, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=27.4774774774775, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790860, Length=219, Percent_Identity=29.2237442922374, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=30.4545454545455, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=31.1926605504587, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=31.7180616740088, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=27.1111111111111, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=30.7017543859649, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=32.1266968325792, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI2367329, Length=237, Percent_Identity=29.535864978903, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=28.9592760180996, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=28.6324786324786, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=26.6968325791855, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26102; Mature: 26102

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIIEDDEVIREELQNFLRKYGYEVNAPIEFNNIIQYVEKENASLILLDINLPEFDG
CCEEEEECCHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
YYICREIRKTSDVPIIIVTSRDSEVDELISMNLGADDFVTKPYNTEILLARITNILKRTY
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GNLKTNNTLNYKDFNLNLSNATLIYKDKSLELTKNEVKILSYLINNRGNIVKRDSLMEYL
CCCCCCCCCCEEEEEEEECCEEEEEECCCCEECHHHHHHHHHHHHCCCCEEEHHHHHHHH
WKSDYFVDDSTLTVNINRLRKKLQEIGIENPIETRRGLGYIMP
HCCCCEECCCEEEEEHHHHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MKKILIIEDDEVIREELQNFLRKYGYEVNAPIEFNNIIQYVEKENASLILLDINLPEFDG
CCEEEEECCHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
YYICREIRKTSDVPIIIVTSRDSEVDELISMNLGADDFVTKPYNTEILLARITNILKRTY
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GNLKTNNTLNYKDFNLNLSNATLIYKDKSLELTKNEVKILSYLINNRGNIVKRDSLMEYL
CCCCCCCCCCEEEEEEEECCEEEEEECCCCEECHHHHHHHHHHHHCCCCEEEHHHHHHHH
WKSDYFVDDSTLTVNINRLRKKLQEIGIENPIETRRGLGYIMP
HCCCCEECCCEEEEEHHHHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA