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The map label for this gene is eutE [H]

Identifier: 226949391

GI number: 226949391

Start: 2391411

End: 2393009

Strand: Reverse

Name: eutE [H]

Synonym: CLM_2316

Alternate gene names: 226949391

Gene position: 2393009-2391411 (Counterclockwise)

Preceding gene: 226949392

Following gene: 226949390

Centisome position: 57.59

GC content: 34.9

Gene sequence:

>1599_bases
ATGGAAAACTTTGATAAGGATTTGCGTTCAATACAACAAGCGAGAGATCTTGCTAGACTTGGAAAAGTAGCAGCTGATAA
AATTGCTGATTATACTGAAGAGCAAATTGACAAAATTTTACGTAATATGGTAAGAGTTGCAGAAGAAAACGCTGTTTGTT
TAGCACAAATGGCAGTTGAAGAGACAGGCTTTGGTAAAGTTGAAGATAAAACTTTCAAAAATCACTTAGCAGCTACTATA
CTTTATAATTCAATTAAAGATATGAAAACTATAGGTGTAATTAATGAAGATAAGGAAAATAAGCTTATAGAACTTGCTGA
ACCAGTTGGCTTAGTTATGGGTATAGTACCTTCAACAAACCCAACTTCTACCGCAATTTTCAAAGCTATGATTTCAATCA
AATCTCGTAATGCTATAGTATTCGCACCACATCCAGCTGCTGCAAAATGTACAATTAAAGCAGTTGAATTAATGAGGGAT
GCAGCAATAGAAGCTGGAGCACCAGAAAACATTATAAGTGCTGTAACTATTCCAACAATTGGAGCTACAAATGAATTAAT
GAAAAGCAAAGAAGTAGCTATAATAATAGCTACAGGTGGTCCAGGAATGGTTAAAGCTGCTTATAGCTCAGGAACACCAG
CTATTGGTGTTGGTGCAGGAAACTCTCCAGCATACATTGAAAGAACTGCTGATGTAGAAAAAGCTGTTAAAAACATTATT
GCAAGTAAAACTTTTGATAATGGTACAATCTGTGCATCAGAACAATCAATAATCTGTGAAGAATGTAATCATGACCTAGT
TGTTGCAGAATTTAAAAAACAAGGTGGATACTTCATGACAGAAGAAGAAACTGAAAAAGTTTGTAAACTATTATTCAAAA
AAGGTAATGCTATGAATGCTAAATTTGTTGGAAGAAGTCCACAAGTTATAGCAAGTGCTGCAGGATTCACAATTCCAGCA
GGAACTAAAGTACTTATAGGAAGACAAAATGGAGTAGGAGAAGGTTACCCATTATCTTTTGAAAAACTTACAACAGTTCT
TGGATTCTATACAGTAAAAGATTGGCATGAAGCATGTGAATTAAGTATTAAATTACTTCAAAATGGAATTGGTCACACAA
TGAGTATCCATACAGAAGATAGAGACATGGTTATGAAGTTTGCTAGAAAACCAGCTTCCCGTATCCTTGTTAACACTGGT
GGAACTCAAGGTGGAACAGGTGCAAGTACAGGTCTTAATCCTTCATTTACTTTAGGATGCGGTACTTGGGGAGGAAGCTC
AGTATCTGAAAATGTTACTCCAAAGCACCTTATGAACATTAAAAGAGTTGCTTATGGTATAAAAGATTGCACAAATTTAG
CATCTAATGACCCAACTTTTAACTGCATAAAAACTGCTGAAAACTGTCATGGAGTTCAAAATCAATTTATGAATATGAGC
CCAGCACAAATTGCAGCAGCTGCAGAAGTTTTAAATAAGGCTAATAATTGCGCTGAAAACACTAATACTTGCACTGAATG
CAGTAATGAAAATACAAAAAATGAAGAACTTTTAGATTTAGTTAATCAAATAGTTGCCGCTATGAAGGGGGCAAACTAA

Upstream 100 bases:

>100_bases
ACAATTTCAAAAGCAGGTAAAAATTTATTAATTAACAAATTTAAATTATATTACGAGAAAAATTAGCTATTTTAAAGGTT
ACGAAAGGAAGTAAGGAATA

Downstream 100 bases:

>100_bases
TGGATAATTGTGAAGAAGTATTAAAACTTTTACTAGAAGCTGTTAAAGGTAAAGAATTAAATAATGCAGTTAATAGAAAT
TCATCTGAGATTCCAGTAGG

Product: acetaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTADVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTMSIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN

Sequences:

>Translated_532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTADVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTMSIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
>Mature_532_residues
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVEETGFGKVEDKTFKNHLAATI
LYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTNPTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRD
AAIEAGAPENIISAVTIPTIGATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTADVEKAVKNII
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNAKFVGRSPQVIASAAGFTIPA
GTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACELSIKLLQNGIGHTMSIHTEDRDMVMKFARKPASRILVNTG
GTQGGTGASTGLNPSFTLGCGTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=430, Percent_Identity=49.0697674418605, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788797, Length=368, Percent_Identity=32.3369565217391, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081896, Length=417, Percent_Identity=22.5419664268585, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 56930; Mature: 56930

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ETGFGKVEDKTFKNHLAATILYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCC
PTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRDAAIEAGAPENIISAVTIPTI
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCC
GATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTADVEKAVKNII
CCHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHH
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNA
HHCCCCCCCEECCCCHHHHHHCCCCEEEEEEHHCCCEEECHHHHHHHHHHHHHCCCCCCE
KFVGRSPQVIASAAGFTIPAGTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACE
EECCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIKLLQNGIGHTMSIHTEDRDMVMKFARKPASRILVNTGGTQGGTGASTGLNPSFTLGC
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEC
GTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENFDKDLRSIQQARDLARLGKVAADKIADYTEEQIDKILRNMVRVAEENAVCLAQMAVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ETGFGKVEDKTFKNHLAATILYNSIKDMKTIGVINEDKENKLIELAEPVGLVMGIVPSTN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCCC
PTSTAIFKAMISIKSRNAIVFAPHPAAAKCTIKAVELMRDAAIEAGAPENIISAVTIPTI
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCC
GATNELMKSKEVAIIIATGGPGMVKAAYSSGTPAIGVGAGNSPAYIERTADVEKAVKNII
CCHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHH
ASKTFDNGTICASEQSIICEECNHDLVVAEFKKQGGYFMTEEETEKVCKLLFKKGNAMNA
HHCCCCCCCEECCCCHHHHHHCCCCEEEEEEHHCCCEEECHHHHHHHHHHHHHCCCCCCE
KFVGRSPQVIASAAGFTIPAGTKVLIGRQNGVGEGYPLSFEKLTTVLGFYTVKDWHEACE
EECCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIKLLQNGIGHTMSIHTEDRDMVMKFARKPASRILVNTGGTQGGTGASTGLNPSFTLGC
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEC
GTWGGSSVSENVTPKHLMNIKRVAYGIKDCTNLASNDPTFNCIKTAENCHGVQNQFMNMS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
PAQIAAAAEVLNKANNCAENTNTCTECSNENTKNEELLDLVNQIVAAMKGAN
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]