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The map label for this gene is dosC [H]

Identifier: 226949305

GI number: 226949305

Start: 2291102

End: 2291650

Strand: Reverse

Name: dosC [H]

Synonym: CLM_2225

Alternate gene names: 226949305

Gene position: 2291650-2291102 (Counterclockwise)

Preceding gene: 226949306

Following gene: 226949304

Centisome position: 55.15

GC content: 24.41

Gene sequence:

>549_bases
ATGAATTATAATCATATGTCCAGGAGTGAATTGATAGAAGAATTAAATAAAAAAGATAAAATAATAAAGAGGATGAAATA
TATTTTTGGAAAAGATCCTGTTACATCCGTGCTTAATAGAAAATTAGGTTTAGAGAAACTTCATAGAGAAATCAAGCTAG
CAAAAATTAATAAAGGTAATTTAATTATAACCTTTGCGGATGTAAATAATCTTAAATATATAAATGATAATTATGGCCAT
GATGCCGGAGATTATGTATTAGAAACTCTATGTAGTATAATAAAAAACAATATAGGAAAAATGGATTTTATTTTTAGATA
TGGGGGAGATGAATTTATAATTGTATTTCTCAAATCTAATATGGATGATGCAAATAAAATATTGTCTAGAATTCAAAAGG
AAATAGATGAATTAGGAAAAAATCTAGAATATAAAATGGGGTTAAGTTATGGTTTAGTAGAGTACAATAAAAATACAAAT
AAAACCATAGAAGATTTAATAAGAATAGCAGATTGTAGGATGTATGAGAATAAAAGAAAAATTGGATAA

Upstream 100 bases:

>100_bases
TCAAAAGATTTTTAAATCATCTCTAAAATGTACAAATTTCGACTGGGTAATATTGTAAAATAATTATGAGCAATATTTAA
ATTAGTAGGAGTGTTTCTTT

Downstream 100 bases:

>100_bases
GACTTTAAAAATTTGTATAATATCATTATACAAATTTTTTTATTTTCGGTGTTAAAATTAAATCTATTTTATATTAAGGT
AAATTATATTTTTAAAAACA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNYNHMSRSELIEELNKKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DAGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKRKIG

Sequences:

>Translated_182_residues
MNYNHMSRSELIEELNKKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DAGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKRKIG
>Mature_182_residues
MNYNHMSRSELIEELNKKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGNLIITFADVNNLKYINDNYGH
DAGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSNMDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTN
KTIEDLIRIADCRMYENKRKIG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=32.5714285714286, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=31.5436241610738, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=28.6486486486486, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI87082007, Length=152, Percent_Identity=26.9736842105263, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=27.1676300578035, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=23.125, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 21267; Mature: 21267

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNHMSRSELIEELNKKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHEEECCCC
LIITFADVNNLKYINDNYGHDAGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSN
EEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECC
MDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTNKTIEDLIRIADCRMYENKRK
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
IG
CC
>Mature Secondary Structure
MNYNHMSRSELIEELNKKDKIIKRMKYIFGKDPVTSVLNRKLGLEKLHREIKLAKINKGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHEEECCCC
LIITFADVNNLKYINDNYGHDAGDYVLETLCSIIKNNIGKMDFIFRYGGDEFIIVFLKSN
EEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECC
MDDANKILSRIQKEIDELGKNLEYKMGLSYGLVEYNKNTNKTIEDLIRIADCRMYENKRK
CCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCC
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]