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The map label for this gene is carA [H]

Identifier: 226949086

GI number: 226949086

Start: 2068654

End: 2069703

Strand: Reverse

Name: carA [H]

Synonym: CLM_2002

Alternate gene names: 226949086

Gene position: 2069703-2068654 (Counterclockwise)

Preceding gene: 226949089

Following gene: 226949085

Centisome position: 49.81

GC content: 31.14

Gene sequence:

>1050_bases
ATGAAAGCAAAGCTTATATTAGAAAATGGAGTAAAATTTAAAGGAAACGCATTTGGATGTCTAAAGGAAAGTGTTGGAGA
GGTTGTATTCAACACTGGATTGACAGGGTATCAAGAAATTCTTACAGACCCATCCTATTATGGTCAGATTGTTACAATGA
CATATCCTCTAATAGGAAACTATGGTATAAATTTAGAAGATATGAAATCAAAATCACCAAAGGTTAGAGGATTTATAGTT
AGAGAAAAATGTAGTTTTTCAAGTAATTTCAGATCAGAAGTAGAATTGGAAGACTATCTAAAGCAAAACAATGTTATAGG
ATTAGAAGGAATAGATACAAGAGCTTTAACTAAGATTTTAAGAAGCAATGGAACTATGAAAGGTATAATAACTTTAGAAG
ATATAGAATATGAAGAAGTTAAAGAAAAACTTAAAGATTTCTCAAATAAAGATGCAGTAGAAAAAGTTACGGTTAAAGAG
AAATATGAAGTGTCAGGAAAAGGAAAGCATGTTGCAATAATGGATTTTGGTATAAAGCAAAATATTGTAAGATCCTTCCT
AAATAGAGGATGCAATGTAACAGTTTTCCCAGCAAATACAAAAGCAGAAGAAGTTTTAAAGGTAAATCCTGATTTAATAT
TTTTATCTAATGGTCCTGGAGACCCAGAAGATTTAACAGAAGTTATAGAAAATATAAAAGTTCTTATAGGTAAAAAGCCT
ATTGTAGGAATATGTTTAGGGCATCAACTTCTAGCTCTAAGCTTAGGAGGAAAGACAGCGGAACTTAAATTTGGTCACAG
AGGATGTAATCATCCTGTTAAGGACCTAAAAGAAAATAAGGTGCATATAACTTCACAAAATCATGGATATTTTGTGGATG
TACTTCCAAAGGATATGGAAGTAACTCATATTAGCGTAAATGATGGAACTATAGAAGGAATGAAACATAAAAATTTATCT
ATATTTTCAGTTCAGTTTCATCCAGAAGCATGTCCAGGTCCAAAGGACAGCGACTATATATTTGATGAATTCATGAAATA
TGCACTATAA

Upstream 100 bases:

>100_bases
TATTTTTTAACATAAATCTTTTTATTTCCCTTTTGTTTTAAAAATTAGGTTTAGCCCTTGCAAAGTGTATTTTTACTGAA
AAAATAAAAGGGGGCCACTT

Downstream 100 bases:

>100_bases
GAACAGGAAATTTAGGAGGTAGCAGTTATGCCATTAAATAAAGATATAAAAAAGGTTTTAGTTATAGGTTCAGGTCCAAT
AGTTATAGGTCAAGCAGCAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKAKLILENGVKFKGNAFGCLKESVGEVVFNTGLTGYQEILTDPSYYGQIVTMTYPLIGNYGINLEDMKSKSPKVRGFIV
REKCSFSSNFRSEVELEDYLKQNNVIGLEGIDTRALTKILRSNGTMKGIITLEDIEYEEVKEKLKDFSNKDAVEKVTVKE
KYEVSGKGKHVAIMDFGIKQNIVRSFLNRGCNVTVFPANTKAEEVLKVNPDLIFLSNGPGDPEDLTEVIENIKVLIGKKP
IVGICLGHQLLALSLGGKTAELKFGHRGCNHPVKDLKENKVHITSQNHGYFVDVLPKDMEVTHISVNDGTIEGMKHKNLS
IFSVQFHPEACPGPKDSDYIFDEFMKYAL

Sequences:

>Translated_349_residues
MKAKLILENGVKFKGNAFGCLKESVGEVVFNTGLTGYQEILTDPSYYGQIVTMTYPLIGNYGINLEDMKSKSPKVRGFIV
REKCSFSSNFRSEVELEDYLKQNNVIGLEGIDTRALTKILRSNGTMKGIITLEDIEYEEVKEKLKDFSNKDAVEKVTVKE
KYEVSGKGKHVAIMDFGIKQNIVRSFLNRGCNVTVFPANTKAEEVLKVNPDLIFLSNGPGDPEDLTEVIENIKVLIGKKP
IVGICLGHQLLALSLGGKTAELKFGHRGCNHPVKDLKENKVHITSQNHGYFVDVLPKDMEVTHISVNDGTIEGMKHKNLS
IFSVQFHPEACPGPKDSDYIFDEFMKYAL
>Mature_349_residues
MKAKLILENGVKFKGNAFGCLKESVGEVVFNTGLTGYQEILTDPSYYGQIVTMTYPLIGNYGINLEDMKSKSPKVRGFIV
REKCSFSSNFRSEVELEDYLKQNNVIGLEGIDTRALTKILRSNGTMKGIITLEDIEYEEVKEKLKDFSNKDAVEKVTVKE
KYEVSGKGKHVAIMDFGIKQNIVRSFLNRGCNVTVFPANTKAEEVLKVNPDLIFLSNGPGDPEDLTEVIENIKVLIGKKP
IVGICLGHQLLALSLGGKTAELKFGHRGCNHPVKDLKENKVHITSQNHGYFVDVLPKDMEVTHISVNDGTIEGMKHKNLS
IFSVQFHPEACPGPKDSDYIFDEFMKYAL

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=359, Percent_Identity=38.4401114206128, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI169790915, Length=358, Percent_Identity=37.4301675977654, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI21361331, Length=358, Percent_Identity=37.4301675977654, Blast_Score=229, Evalue=2e-60,
Organism=Escherichia coli, GI1786215, Length=368, Percent_Identity=46.7391304347826, Blast_Score=328, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI193204318, Length=366, Percent_Identity=39.6174863387978, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=366, Percent_Identity=42.6229508196721, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=387, Percent_Identity=36.1757105943152, Blast_Score=235, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6322638, Length=184, Percent_Identity=28.2608695652174, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45555749, Length=369, Percent_Identity=40.650406504065, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24642586, Length=369, Percent_Identity=40.650406504065, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 38930; Mature: 38930

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKLILENGVKFKGNAFGCLKESVGEVVFNTGLTGYQEILTDPSYYGQIVTMTYPLIGN
CCCEEEECCCCEECCCCHHHHHHHHHHHEECCCCCHHHHHHCCCHHHCEEEEEEEEEECC
YGINLEDMKSKSPKVRGFIVREKCSFSSNFRSEVELEDYLKQNNVIGLEGIDTRALTKIL
CCCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHH
RSNGTMKGIITLEDIEYEEVKEKLKDFSNKDAVEKVTVKEKYEVSGKGKHVAIMDFGIKQ
HCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHEEEHHHEEEECCCCCEEEEEECCHHH
NIVRSFLNRGCNVTVFPANTKAEEVLKVNPDLIFLSNGPGDPEDLTEVIENIKVLIGKKP
HHHHHHHHCCCCEEEEECCCCCCHHEEECCCEEEEECCCCCHHHHHHHHHHHHEEECCCC
IVGICLGHQLLALSLGGKTAELKFGHRGCNHPVKDLKENKVHITSQNHGYFVDVLPKDME
EEEEEECCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCEEEEEECCCCEEEEECCCCCE
VTHISVNDGTIEGMKHKNLSIFSVQFHPEACPGPKDSDYIFDEFMKYAL
EEEEEECCCEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKAKLILENGVKFKGNAFGCLKESVGEVVFNTGLTGYQEILTDPSYYGQIVTMTYPLIGN
CCCEEEECCCCEECCCCHHHHHHHHHHHEECCCCCHHHHHHCCCHHHCEEEEEEEEEECC
YGINLEDMKSKSPKVRGFIVREKCSFSSNFRSEVELEDYLKQNNVIGLEGIDTRALTKIL
CCCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHH
RSNGTMKGIITLEDIEYEEVKEKLKDFSNKDAVEKVTVKEKYEVSGKGKHVAIMDFGIKQ
HCCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHEEEHHHEEEECCCCCEEEEEECCHHH
NIVRSFLNRGCNVTVFPANTKAEEVLKVNPDLIFLSNGPGDPEDLTEVIENIKVLIGKKP
HHHHHHHHCCCCEEEEECCCCCCHHEEECCCEEEEECCCCCHHHHHHHHHHHHEEECCCC
IVGICLGHQLLALSLGGKTAELKFGHRGCNHPVKDLKENKVHITSQNHGYFVDVLPKDME
EEEEEECCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCEEEEEECCCCEEEEECCCCCE
VTHISVNDGTIEGMKHKNLSIFSVQFHPEACPGPKDSDYIFDEFMKYAL
EEEEEECCCEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]