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The map label for this gene is hupS [C]

Identifier: 226949082

GI number: 226949082

Start: 2062315

End: 2063187

Strand: Reverse

Name: hupS [C]

Synonym: CLM_1998

Alternate gene names: 226949082

Gene position: 2063187-2062315 (Counterclockwise)

Preceding gene: 226949085

Following gene: 226949081

Centisome position: 49.65

GC content: 33.1

Gene sequence:

>873_bases
ATGAATAATAGCTTTTGCCCATTAATTGAAAGGAAAGATACCACTTCAAAAATGCTTTGCAAAGAAGCTATGGAATCAAT
TAATCATAAAAAAACAAAAAAAATAAATGCTATATGGCTAGAAGTAACAGGCTGTTCCGGAAATATAATTTCTCTTTTGA
ACGCAGAAAATCCAGGGCTTATTTATATACTTAGAAATATAGTTAATTTAACTTTTAATAATTCTTTAATGGGAGCTGAA
GGAGAATTTGCATATGAACAGTTTTTAAATACCTTGGACACAGAATTTATATTATTAGTAGATGGAGCTGTGTCCACTAA
AGAAAATGGCTATTATAACATTATTGCTAATTATAAAGGAAAACCTGTTACCGCATTAGAAGCAATAAAAATGGCAGGTG
AAAAAGCAAAATATGTGGTAACTGTAGGAACCTGTTCTTCTTATGGTGGTATTTCTGCAGCTAAGCCTAATCCTTCTGGT
AGCAAAAGCGTAGACGCTGTTATAAATAAAGAAGTAATAAAATTACCAGGGTGTCCTTGTCATCCTGACTGGGTGGTAGG
TACTTTAGCTCATCTAGTTACTTACGGTAAACCAGAATTAGATAATAAAGGGAGACCTATATTATTTTATGGAATAACTA
TTCATGATAGTTGTACAAGAAGAGGCTTTTTTGATAAAAGAATCTTTGCCAAAAAATTTGGAGAAGAAGGATGTATGTTT
AAGTTAGGATGTAGAGGACCTGTTACAAAAACAGATTGCCCCAGAAGAAAATGGAATGGTTATGTAAATTGGCCTATTGG
AGACAACACAAATTGTATAGGCTGTGCCCAAGAAAGATTTCCAGATGGTATGGAGCCCTTTGTTAGGTATTAA

Upstream 100 bases:

>100_bases
ACTTTATTTATGTTCCAATAGCATAATAAATAATTTTTATTTATATAATTTATTGGAGTATATTTTTTATATGGTAATTA
ATAAATTTTTAGGAGGAGTA

Downstream 100 bases:

>100_bases
TTTCTATAGTTTAAAAAATAAAGTATATTAATTTTTTAGTTTAAATTATTTATGCTTAAGATAACTAAGTAAACTTTGAA
TTATAAAAATGGGAGGTCAT

Product: [Ni/Fe] hydrogenase small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNNSFCPLIERKDTTSKMLCKEAMESINHKKTKKINAIWLEVTGCSGNIISLLNAENPGLIYILRNIVNLTFNNSLMGAE
GEFAYEQFLNTLDTEFILLVDGAVSTKENGYYNIIANYKGKPVTALEAIKMAGEKAKYVVTVGTCSSYGGISAAKPNPSG
SKSVDAVINKEVIKLPGCPCHPDWVVGTLAHLVTYGKPELDNKGRPILFYGITIHDSCTRRGFFDKRIFAKKFGEEGCMF
KLGCRGPVTKTDCPRRKWNGYVNWPIGDNTNCIGCAQERFPDGMEPFVRY

Sequences:

>Translated_290_residues
MNNSFCPLIERKDTTSKMLCKEAMESINHKKTKKINAIWLEVTGCSGNIISLLNAENPGLIYILRNIVNLTFNNSLMGAE
GEFAYEQFLNTLDTEFILLVDGAVSTKENGYYNIIANYKGKPVTALEAIKMAGEKAKYVVTVGTCSSYGGISAAKPNPSG
SKSVDAVINKEVIKLPGCPCHPDWVVGTLAHLVTYGKPELDNKGRPILFYGITIHDSCTRRGFFDKRIFAKKFGEEGCMF
KLGCRGPVTKTDCPRRKWNGYVNWPIGDNTNCIGCAQERFPDGMEPFVRY
>Mature_290_residues
MNNSFCPLIERKDTTSKMLCKEAMESINHKKTKKINAIWLEVTGCSGNIISLLNAENPGLIYILRNIVNLTFNNSLMGAE
GEFAYEQFLNTLDTEFILLVDGAVSTKENGYYNIIANYKGKPVTALEAIKMAGEKAKYVVTVGTCSSYGGISAAKPNPSG
SKSVDAVINKEVIKLPGCPCHPDWVVGTLAHLVTYGKPELDNKGRPILFYGITIHDSCTRRGFFDKRIFAKKFGEEGCMF
KLGCRGPVTKTDCPRRKWNGYVNWPIGDNTNCIGCAQERFPDGMEPFVRY

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=259, Percent_Identity=32.8185328185328, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1789371, Length=285, Percent_Identity=34.7368421052632, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 32137; Mature: 32137

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00559 MOLYBDOPTERIN_EUK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSFCPLIERKDTTSKMLCKEAMESINHKKTKKINAIWLEVTGCSGNIISLLNAENPGL
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEEECCCCCE
IYILRNIVNLTFNNSLMGAEGEFAYEQFLNTLDTEFILLVDGAVSTKENGYYNIIANYKG
EEEEHHHHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCC
KPVTALEAIKMAGEKAKYVVTVGTCSSYGGISAAKPNPSGSKSVDAVINKEVIKLPGCPC
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCC
HPDWVVGTLAHLVTYGKPELDNKGRPILFYGITIHDSCTRRGFFDKRIFAKKFGEEGCMF
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHCCCCCCEE
KLGCRGPVTKTDCPRRKWNGYVNWPIGDNTNCIGCAQERFPDGMEPFVRY
EECCCCCCCCCCCCHHHCCCEEECCCCCCCCEEEECHHHCCCCCHHHHCC
>Mature Secondary Structure
MNNSFCPLIERKDTTSKMLCKEAMESINHKKTKKINAIWLEVTGCSGNIISLLNAENPGL
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEEECCCCCE
IYILRNIVNLTFNNSLMGAEGEFAYEQFLNTLDTEFILLVDGAVSTKENGYYNIIANYKG
EEEEHHHHHEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCC
KPVTALEAIKMAGEKAKYVVTVGTCSSYGGISAAKPNPSGSKSVDAVINKEVIKLPGCPC
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCC
HPDWVVGTLAHLVTYGKPELDNKGRPILFYGITIHDSCTRRGFFDKRIFAKKFGEEGCMF
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCHHHCCCHHHHHHHHHCCCCCCEE
KLGCRGPVTKTDCPRRKWNGYVNWPIGDNTNCIGCAQERFPDGMEPFVRY
EECCCCCCCCCCCCHHHCCCEEECCCCCCCCEEEECHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]