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The map label for this gene is rluB [H]

Identifier: 226949054

GI number: 226949054

Start: 2033129

End: 2033839

Strand: Reverse

Name: rluB [H]

Synonym: CLM_1970

Alternate gene names: 226949054

Gene position: 2033839-2033129 (Counterclockwise)

Preceding gene: 226949055

Following gene: 226949053

Centisome position: 48.95

GC content: 26.3

Gene sequence:

>711_bases
ATGGAAGAAAGATTACAAAAATATATGGCCTCTTGTGGAGTTGCTTCTAGAAGAAAATGTGAGGATATTATTTTAGAAGG
GCGAGTAGAAGTAAATGGTATTAGAATAAAAGAATTAGGTACAAAAATTATATCTGGAGAAGACATTATAAAAGTAGATG
GAAATATCATAGAACCAGAAGAAAATAAAATTTACATTTTACTTAATAAACCAAAAGGATACGTATGTACTGCTAAAGAT
GAAAAGGACAGAAAAACTATTTTAGATTTAGTAAAAGTTAAAGAAAGAATATACCCTATAGGTAGGTTAGATATGGATAC
ATCAGGTATAATAATACTAACCAATGACGGGGAAATATACAATAAGATTATTCATCCTAAATTTGAAAAAATAAAGACTT
ATAAGGCTAAAGTAAAAGGTACTATAAAAAAAGACCATATAGAGTTATTTAAAAAAGGTATAGATATAGGAGATTACATA
ACAGCTCCAGCAACATTAGAAATTTTGAAAATAAGTAAGGATACTTCTGAAATAATAATAAAAATTCACGAAGGTAAAAA
TAGACAAGTAAGAAGAATGTGTAAATCTATAGGTCATGCTGTTATAGATTTAGATAGGATTTCTATAGGTAATATAACAA
AAAATGATTTGAAATTAGGTGAATGGAGATATTTAACTAAGAAGGAAATAGATTATATAACAAAGATATAA

Upstream 100 bases:

>100_bases
AAGTTCGTCGTGATCTCCTTATATTTTGACTTTTTACATAAAACCTATACAATAGTATTGTTAGTATTGTTACAACAAAA
TATAATATAAAGGTGATTGT

Downstream 100 bases:

>100_bases
ATTAAATCTAAAAGCAAATAAGGCTGTTAAGAACTCACCAATAATATATTTAAAATCTTTTAAGATTCATTAAAAAATGA
TAAATAATTTCAAAAATATA

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIRIKELGTKIISGEDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKDRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYI
TAPATLEILKISKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKNDLKLGEWRYLTKKEIDYITKI

Sequences:

>Translated_236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIRIKELGTKIISGEDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKDRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYI
TAPATLEILKISKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKNDLKLGEWRYLTKKEIDYITKI
>Mature_236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIRIKELGTKIISGEDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKDRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYI
TAPATLEILKISKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKNDLKLGEWRYLTKKEIDYITKI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=37.9912663755458, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=34.051724137931, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=32.3886639676113, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=32.9545454545455, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27069; Mature: 27069

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIRIKELGTKIISGEDIIKVDGNIIEPE
CHHHHHHHHHHCCCCCHHHHHHHEEECEEEECCEEEHHHCCEEECCCCEEEECCEEECCC
ENKIYILLNKPKGYVCTAKDEKDRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIY
CCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHH
NKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYITAPATLEILKISKDTSEIII
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCCEEEE
KIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKNDLKLGEWRYLTKKEIDYITKI
EEECCCCHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCCCEEECCHHHHHHHHCC
>Mature Secondary Structure
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIRIKELGTKIISGEDIIKVDGNIIEPE
CHHHHHHHHHHCCCCCHHHHHHHEEECEEEECCEEEHHHCCEEECCCCEEEECCEEECCC
ENKIYILLNKPKGYVCTAKDEKDRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIY
CCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHH
NKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYITAPATLEILKISKDTSEIII
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCEEEEEEECCCCCEEEE
KIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKNDLKLGEWRYLTKKEIDYITKI
EEECCCCHHHHHHHHHHCCEEEEEECEEECCCCCCCCCCCCEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]