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The map label for this gene is miaB [H]

Identifier: 226949042

GI number: 226949042

Start: 2018977

End: 2020329

Strand: Reverse

Name: miaB [H]

Synonym: CLM_1958

Alternate gene names: 226949042

Gene position: 2020329-2018977 (Counterclockwise)

Preceding gene: 226949046

Following gene: 226949041

Centisome position: 48.62

GC content: 29.49

Gene sequence:

>1353_bases
GTGAATGAAATATTAAATACAAAAGATATAAATGTTATAGGTGAATTTTTCATAGAAACCTGGGGTTGCCAAATGAATGA
AGAGGATTCAGAAAAACTTTCAGGTATGTTGAAAAAAGAGGGATATATAAGGACTGAAGAAAGAGAAAATGCAGATGTAA
TTATATTCAACACCTGCTGTGTTAGAGAAAATGCAGAACTTAAAGTATATGGTAATTTAGGGATACTTAAAGGTTTAAAA
TCTAAAAACCCAAATTTAATTATAGCTGTAACTGGTTGCATGATGCAACAAAAAGGTATGGCAGAAACCATTAAAAAGAA
ATTTCCTTTTGTAGATATTATAATAGGAACTCATAATCTGCATAACTTCCCAAATTATTTAAATGAAGTTAAGAAAAAAG
ATACATCTGTATTAAAGATACAAGAAAAAGAAAATAGCATAATAGAAAATATGCCAATAGATAGAAAAAATAGTATGAAA
GCTTTTGTTACTATAATGTATGGTTGCAATAATTTCTGTACTTATTGTATAGTTCCTTATGTAAGAGGAAGGGAAAGAAG
CAGAACCCCTGAAAATATAGAAGCTGAAATAAAGAAACTAATTTCAGAAGATTATAAGGAAATAACTCTACTTGGCCAAA
ATGTAAATTCCTATGGTAAGGATTTAGAACCTAATGTTACATTTGCAGAGCTTTTAAAAAGAGTAAATAATATAGAAGGA
TTAGAAAGAGTAAGATTTATGACTTCCCATCCAAAGGACTTAACAGATGATGTTATAGAAGCTATTGCTAAATGTGATAA
ACTGTGTGAACAAATACATCTTCCTGTACAATCCGGTTCTAGTGAAATATTGAAAAAAATGAATAGACATTATGATAGAG
AAAAATATTTAGATGTAGTATCCAAAATAAAAAAACTTATACCTAACGTAGCACTTTCTACTGATATAATAGTAGGATTC
CCTGGAGAAACAGAAAAAGACTTTGAAAAAACATTAAGTTTAGTAAAGGAAGTGGAATACGACTCTGCTTTTACCTTCTT
ATATTCCATAAGAAAGGGAACCCCAGCTGCTAAATTTGAGGATCAAGTACCTGAAGATGTTAAACACAAGAGATTTAATA
GATTAGTAGAAGTGCTTAATGAAATAAGTGCTAAAAAGAACAAAGCCTATGAAGGAAAAATAGAAGAAGTTTTAGTAGAG
GGTACTAGTAAAAATGACGAAAATAAATTAATGGGTAGAACAAGAACAGGAAAACTTGTTAACTTTATTGGCGATAAAGA
CTCCATAGGAGAGCTTGTTAATGTTAAAATAATAAAAGCGAACTCTTTTTCATTAACAGGGGAAGAAATTTAA

Upstream 100 bases:

>100_bases
ATAACTTACTTTATATTGTGCAGTATACCATTAGAAATTATTAATAAAATATAGTATAATGTATAGGTCAAATTTTTTAA
ATTTATGAGGTGAATAAATA

Downstream 100 bases:

>100_bases
ATATAAATTAAAAAGCTCTTGAATAAGAGCTTTTTTTAAAAGATTAAATTTAAAAGGAATATTTTAAGGAGGAGAAAATA
TGGGATTAACTCCAATGATG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEDYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEKTLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI

Sequences:

>Translated_450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEDYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEKTLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI
>Mature_450_residues
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGLK
SKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMK
AFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEIKKLISEDYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDIIVGF
PGETEKDFEKTLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVE
GTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVKIIKANSFSLTGEEI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=490, Percent_Identity=37.7551020408163, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI28872784, Length=490, Percent_Identity=37.7551020408163, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=29.8440979955457, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=435, Percent_Identity=43.2183908045977, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=28.7946428571429, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=36.6666666666667, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71996771, Length=336, Percent_Identity=25, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=36.6255144032922, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI19922432, Length=442, Percent_Identity=29.8642533936652, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51354; Mature: 51354

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
VRGRERSRTPENIEAEIKKLISEDYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
HCCCCCCCCCCCHHHHHHHHHHHHHHHEEHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEKTLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGELVNVKIIKANSFSLTGEEI
EECCCCHHHCCEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNEILNTKDINVIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEE
VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL
ECCCCCEEEEECCHHHHCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCEEEEEEECCCC
HNFPNYLNEVKKKDTSVLKIQEKENSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY
CCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
VRGRERSRTPENIEAEIKKLISEDYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG
HCCCCCCCCCCCHHHHHHHHHHHHHHHEEHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCH
LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHH
SKIKKLIPNVALSTDIIVGFPGETEKDFEKTLSLVKEVEYDSAFTFLYSIRKGTPAAKFE
HHHHHHCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
DQVPEDVKHKRFNRLVEVLNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCHH
NFIGDKDSIGELVNVKIIKANSFSLTGEEI
EECCCCHHHCCEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA