Click here to switch to the map view.

The map label for this gene is lplA [H]

Identifier: 226948962

GI number: 226948962

Start: 1917224

End: 1918219

Strand: Direct

Name: lplA [H]

Synonym: CLM_1876

Alternate gene names: 226948962

Gene position: 1917224-1918219 (Clockwise)

Preceding gene: 226948961

Following gene: 226948963

Centisome position: 46.14

GC content: 25.1

Gene sequence:

>996_bases
ATGCAGTTTTTAGTAAATAAATCAACAAATCCTTTTTTCAATTTAGCTTTAGAAGAATATCTTTTAAAAAATGTAGATAT
TAAGGAAGATTATTTTATTCTCTGGCAAAATGAGCCTACAATTGTAATAGGAAAGCATCAAAATACATTAAAGGAAATTA
ATATGAATTTTGTGCAAGATAATAATATAAATGTAGTTAGAAGGAATTCTGGAGGAGGAGCTGTATATCACGATTTAGGT
AATATAAATTTTACCTTCATAACTAAATATGATGAAAAACATCTTCTTGATTTTAAAACTTTTACTAATCCTGTGGTTTA
TTCATTAGGCAAACTCAATGTAAAGGCAGAGTTATCAGGAAGAAATGATATTTTAATAGATGGAAGAAAAATATCAGGAA
ATTCTCAACACATATATAAGGATAGATTTCTTCATCATGGAACTTTATTATTTAACTCTGAATTAGAAAACTTAGTAAAA
GCTTTAAATGTAGATAATGATAAGATTTTATCTAAGGGTATTGAATCAATAAAAAGTAGAGTAACAAATATTAAAGAACA
TGTAAAAAAAGATATTTCTATGGAGAAATTTAAAGAAATACTTATAGAAAATATTTTTATATGGAATAAAAATTCCCTTA
AAGAGTACAATTTAACCAATGACCACATTAATGATATAGAAAAATTAATGGAAGAAAAATATATGACATGGCAGTGGAAT
TACGGGTATTCACCAGAGTTTAATTATAGAAATTCTAAAAGATTTCAAGGTGGCAAGCTTGAAGTATTATTAAATATTGT
AGAAGGACATATTAATGAATGCAAAATATATGGTGACTTTTTAGGATTAATGGATGTATCAGAAATAGAGAAAAGGATTA
TTGGGATAAAATATGGAGAAGAATATATAGATGAATTTTTAAGAGAAATTGATATAAAGAAGTATTTTGGTACTCTATGT
TTTGATGAAATAAAATCCTGTTTCGTAGAACTTTAA

Upstream 100 bases:

>100_bases
TCCCACAATAGGAGAAGCGATGAAGGAGGCAGCTCTTGCAGTAAACAAAGAAGCAATTCATATGGTGAATAAATAAAATC
AAGTTATGGAGGTAAAAGTA

Downstream 100 bases:

>100_bases
AAGCCTATTTATTTAGTTAATTTTTAAATGTTATTTCGAAAAGCACAGAATATATATTTATACCACAAGTATAATATAAT
ACATTGTTACAAAACATTAA

Product: putative lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKKDISMEKFKEILIENIFIWNKNSLKEYNLTNDHINDIEKLMEEKYMTWQWN
YGYSPEFNYRNSKRFQGGKLEVLLNIVEGHINECKIYGDFLGLMDVSEIEKRIIGIKYGEEYIDEFLREIDIKKYFGTLC
FDEIKSCFVEL

Sequences:

>Translated_331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKKDISMEKFKEILIENIFIWNKNSLKEYNLTNDHINDIEKLMEEKYMTWQWN
YGYSPEFNYRNSKRFQGGKLEVLLNIVEGHINECKIYGDFLGLMDVSEIEKRIIGIKYGEEYIDEFLREIDIKKYFGTLC
FDEIKSCFVEL
>Mature_331_residues
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQDNNINVVRRNSGGGAVYHDLG
NINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSGRNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVK
ALNVDNDKILSKGIESIKSRVTNIKEHVKKDISMEKFKEILIENIFIWNKNSLKEYNLTNDHINDIEKLMEEKYMTWQWN
YGYSPEFNYRNSKRFQGGKLEVLLNIVEGHINECKIYGDFLGLMDVSEIEKRIIGIKYGEEYIDEFLREIDIKKYFGTLC
FDEIKSCFVEL

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=312, Percent_Identity=31.4102564102564, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729884, Length=312, Percent_Identity=31.4102564102564, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729882, Length=312, Percent_Identity=31.4102564102564, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729880, Length=312, Percent_Identity=31.4102564102564, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729878, Length=312, Percent_Identity=31.4102564102564, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1790846, Length=311, Percent_Identity=34.4051446945338, Blast_Score=207, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=31.496062992126, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322415, Length=291, Percent_Identity=30.2405498281787, Blast_Score=112, Evalue=6e-26,
Organism=Drosophila melanogaster, GI28573464, Length=293, Percent_Identity=29.3515358361775, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38900; Mature: 38900

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQD
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHHHCCCEEEEC
NNINVVRRNSGGGAVYHDLGNINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSG
CCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCEEEHHHHCCHHHHHHCCEEEEEEECC
RNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVKALNVDNDKILSKGIESIKSR
CCCEEEECEEECCCCCEEHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTNIKEHVKKDISMEKFKEILIENIFIWNKNSLKEYNLTNDHINDIEKLMEEKYMTWQWN
HHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEC
YGYSPEFNYRNSKRFQGGKLEVLLNIVEGHINECKIYGDFLGLMDVSEIEKRIIGIKYGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHEEEECCH
EYIDEFLREIDIKKYFGTLCFDEIKSCFVEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQFLVNKSTNPFFNLALEEYLLKNVDIKEDYFILWQNEPTIVIGKHQNTLKEINMNFVQD
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHHHCCCEEEEC
NNINVVRRNSGGGAVYHDLGNINFTFITKYDEKHLLDFKTFTNPVVYSLGKLNVKAELSG
CCEEEEEECCCCCEEEEECCCEEEEEEEECCCCCEEEHHHHCCHHHHHHCCEEEEEEECC
RNDILIDGRKISGNSQHIYKDRFLHHGTLLFNSELENLVKALNVDNDKILSKGIESIKSR
CCCEEEECEEECCCCCEEHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTNIKEHVKKDISMEKFKEILIENIFIWNKNSLKEYNLTNDHINDIEKLMEEKYMTWQWN
HHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEC
YGYSPEFNYRNSKRFQGGKLEVLLNIVEGHINECKIYGDFLGLMDVSEIEKRIIGIKYGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHEEEECCH
EYIDEFLREIDIKKYFGTLCFDEIKSCFVEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]