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The map label for this gene is spaK [H]

Identifier: 226948917

GI number: 226948917

Start: 1856445

End: 1857824

Strand: Direct

Name: spaK [H]

Synonym: CLM_1827

Alternate gene names: 226948917

Gene position: 1856445-1857824 (Clockwise)

Preceding gene: 226948916

Following gene: 226948918

Centisome position: 44.68

GC content: 27.54

Gene sequence:

>1380_bases
ATGGGTATAAATAAAAAACAGGTCACATTAAAAACTGTTTTTTTTAGATATCTATTAGCATTAGCTCTTGCTTTTTTTGT
AGCAATAATAATTGAAATAATTATAACAAGCTTAGGGATGCAAATGAATTTTTTTAATAGTGCTAATTATAGTGAAGATT
TAGCAAGACGAGCAAAGCCCATACTTCAATCTTCAGAAAGAATTACAGAAGACATGATTCCTAAGGGATGTAAATTTGCT
GTATTTGATAAAGAATTTAAGGTCATTAAAACAGATCTTAAGGGGGAAAATTTAAAACAAGCAACTTTATATGCTAAAGG
CATTGATAGAAAAAATGGAGATAAGAAAAGTTATTACTTTATTGAAAGAAAAGATGGCTTTTGTGTATTGCAATATTATA
TCAAAATGAGCTATTCTTCAGAATGGATGAATGAACATTTTCCTAATCCAGAATTTTTATTAATTGCAACTCTTATTTTG
GGATGTTTAATTGGAGCTTTTATTATTTCTATAGCTTTTGCAAAAAATTTAAAAGATAATTTAATTCCTTTAATGGAGGC
TACTGAAAAAATAAAAGAGCAGGATTTAGATTTTGACATTGGAAATTCTCCTATAAAGGAATTTAATGATGTGTTATGTT
CTATTTCTGATATGAAAGAGGAATTAAAAAAATCTCTAAAAGAGCAGTGGAATTTAGAGAGGACTAAAAAAGAGCAGATA
TCTTCTTTAGCTCATGATATTAAAACTCCTTTAACTATTATTAAAGGAAATACTGAACTTTTAAAAGATTCTGATCTTAA
TAATGAACAGAAAGAATATATTGGGTTTATCGAAAAGAATTCAATACAGATTGAAAAGTATATAAAATTGCTTATTTATA
TGTCAAAAGGTAAATTAGAGTTTTTAGGAAAATTACAGAAAACTAATACAAAGGAATTTGTTCATGAGATTTATAATCAA
CTAATTGCCCTTGCGGGGCCAAAGGAACTACAAATTAAATTCGAACAAAAAGATATACCAGAAAATATAATTATAAATAA
AGAAGCTATGCATAGAGCTATAATAAATGTAATCTCTAATGCAGTGGATTATTCACCTTCTAATAGTGAATTATATTTTT
ATGTTGAAGGTAAAAATGACTCTGTAGAGTTTTCGGTTATTGACAGTGGTAAAGGATTCTCTAGTGAGGATTTAAAAGAT
GCTAAGAAACAGTTCTATATGGGAGATTCAAGTAGAACGTCAAAAACACATTGGGGTATGGGGCTTTATATTGCAGATTC
TATTGTAAAACAACATAATGGAGTTTTGACTCTTAAAAATTCACATAAAATAGGAGGTGCAAAGGTAATAATTAAAATAC
CTTTATTAAGTGACTTTTAG

Upstream 100 bases:

>100_bases
GTGATAGCTCTGCTATTACAGAGCATATTAAAAATATTAGAGCCAAGTTAAAAGTCTTTGATATGTCACCAATAGAAACA
GTCTGGGGGATTGGTTATAA

Downstream 100 bases:

>100_bases
GTTTGCTAAAGGTCATTTAGAATAATTATCTATAAATAGAAATGGCACAAAAATAAATATTATAAAAATAAATCACTAAA
AATTAATTAGAGTTCTGATT

Product: putative lantibiotic biosynthesis sensor histidine kinase

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIITSLGMQMNFFNSANYSEDLARRAKPILQSSERITEDMIPKGCKFA
VFDKEFKVIKTDLKGENLKQATLYAKGIDRKNGDKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLIATLIL
GCLIGAFIISIAFAKNLKDNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLERTKKEQI
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIGFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFVHEIYNQ
LIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPSNSELYFYVEGKNDSVEFSVIDSGKGFSSEDLKD
AKKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF

Sequences:

>Translated_459_residues
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIITSLGMQMNFFNSANYSEDLARRAKPILQSSERITEDMIPKGCKFA
VFDKEFKVIKTDLKGENLKQATLYAKGIDRKNGDKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLIATLIL
GCLIGAFIISIAFAKNLKDNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLERTKKEQI
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIGFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFVHEIYNQ
LIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPSNSELYFYVEGKNDSVEFSVIDSGKGFSSEDLKD
AKKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
>Mature_458_residues
GINKKQVTLKTVFFRYLLALALAFFVAIIIEIIITSLGMQMNFFNSANYSEDLARRAKPILQSSERITEDMIPKGCKFAV
FDKEFKVIKTDLKGENLKQATLYAKGIDRKNGDKKSYYFIERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLIATLILG
CLIGAFIISIAFAKNLKDNLIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLERTKKEQIS
SLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIGFIEKNSIQIEKYIKLLIYMSKGKLEFLGKLQKTNTKEFVHEIYNQL
IALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISNAVDYSPSNSELYFYVEGKNDSVEFSVIDSGKGFSSEDLKDA
KKQFYMGDSSRTSKTHWGMGLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=253, Percent_Identity=26.4822134387352, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786783, Length=346, Percent_Identity=22.5433526011561, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=24.2290748898678, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=21.9512195121951, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=28.110599078341, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=25.4464285714286, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=25.6637168141593, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=21.484375, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52493; Mature: 52361

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGINKKQVTLKTVFFRYLLALALAFFVAIIIEIIITSLGMQMNFFNSANYSEDLARRAKP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHH
ILQSSERITEDMIPKGCKFAVFDKEFKVIKTDLKGENLKQATLYAKGIDRKNGDKKSYYF
HHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEE
IERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLIATLILGCLIGAFIISIAFAKNLKDN
EECCCCEEEEEEHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLERTKKEQI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIGFIEKNSIQIEKYIKLLIYMSKGKLE
HHHHHHHCCCEEEEECCHHHCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHCCCCHH
FLGKLQKTNTKEFVHEIYNQLIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISN
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
AVDYSPSNSELYFYVEGKNDSVEFSVIDSGKGFSSEDLKDAKKQFYMGDSSRTSKTHWGM
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
GLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
GINKKQVTLKTVFFRYLLALALAFFVAIIIEIIITSLGMQMNFFNSANYSEDLARRAKP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHH
ILQSSERITEDMIPKGCKFAVFDKEFKVIKTDLKGENLKQATLYAKGIDRKNGDKKSYYF
HHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEE
IERKDGFCVLQYYIKMSYSSEWMNEHFPNPEFLLIATLILGCLIGAFIISIAFAKNLKDN
EECCCCEEEEEEHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LIPLMEATEKIKEQDLDFDIGNSPIKEFNDVLCSISDMKEELKKSLKEQWNLERTKKEQI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SSLAHDIKTPLTIIKGNTELLKDSDLNNEQKEYIGFIEKNSIQIEKYIKLLIYMSKGKLE
HHHHHHHCCCEEEEECCHHHCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHHCCCCHH
FLGKLQKTNTKEFVHEIYNQLIALAGPKELQIKFEQKDIPENIIINKEAMHRAIINVISN
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
AVDYSPSNSELYFYVEGKNDSVEFSVIDSGKGFSSEDLKDAKKQFYMGDSSRTSKTHWGM
HHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
GLYIADSIVKQHNGVLTLKNSHKIGGAKVIIKIPLLSDF
HHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]