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The map label for this gene is spaR [H]

Identifier: 226948916

GI number: 226948916

Start: 1855795

End: 1856454

Strand: Direct

Name: spaR [H]

Synonym: CLM_1826

Alternate gene names: 226948916

Gene position: 1855795-1856454 (Clockwise)

Preceding gene: 226948915

Following gene: 226948917

Centisome position: 44.66

GC content: 31.06

Gene sequence:

>660_bases
ATGTCAAAAATACTTGTAATTGATGATGAAGAAGATATTTTGATTCTTATTAAAAATGTTCTTTCCAAAGATAATCATTT
AGTAACTACAGTAAAAGAAGCAAAAGATATTCCATTAAATGATTTTGTAAAATTTGATTTGATTTTACTTGATGTAATGA
TGCCTGATATTGATGGTTTTACTTTATGTAAGGAAATTAGAGAGATTGTAGACTGTCCAATTCTTTTCTTAACAGCAAAA
ACTATGGAAAGTGACATTATGTTTGGATTAGGAATTGGTGGGGATGATTATATAACAAAACCTTTTGGAGTAGGAGAATT
AAGGGCTAGAGTAAATGCTCATCTGCGCAGGGAACATCGTGAAAAACGTCATATTTTTGTAGCTTCAGATATTATATTTA
ATCTATCCAGTAAGGAAATTAAAATAGGCGATGTAAAGGTGCCCTTTACAAAGGGTGAATATACTATATGTGAATACCTT
GCAAGAAATTCAGGACAGGTGTTTTCTAAAGAACAAATATATGAAGCGGTTTATGGGTATGATGGAGAAAGTGATAGCTC
TGCTATTACAGAGCATATTAAAAATATTAGAGCCAAGTTAAAAGTCTTTGATATGTCACCAATAGAAACAGTCTGGGGGA
TTGGTTATAAATGGGTATAA

Upstream 100 bases:

>100_bases
AAATAGAAAATTTCAGTAATTATCAATAACAAGCTGTATTTTTTTCTAATAGGGTAGTTTATAATTAAAAATGAAGATGT
TATAAAGATAGGAGGATATT

Downstream 100 bases:

>100_bases
ATAAAAAACAGGTCACATTAAAAACTGTTTTTTTTAGATATCTATTAGCATTAGCTCTTGCTTTTTTTGTAGCAATAATA
ATTGAAATAATTATAACAAG

Product: putative lantibiotic biosynthesis regulatory protein

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSKILVIDDEEDILILIKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREIVDCPILFLTAK
TMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVPFTKGEYTICEYL
ARNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDMSPIETVWGIGYKWV

Sequences:

>Translated_219_residues
MSKILVIDDEEDILILIKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREIVDCPILFLTAK
TMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVPFTKGEYTICEYL
ARNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDMSPIETVWGIGYKWV
>Mature_218_residues
SKILVIDDEEDILILIKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGFTLCKEIREIVDCPILFLTAKT
MESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHREKRHIFVASDIIFNLSSKEIKIGDVKVPFTKGEYTICEYLA
RNSGQVFSKEQIYEAVYGYDGESDSSAITEHIKNIRAKLKVFDMSPIETVWGIGYKWV

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=35.5855855855856, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=31.2217194570136, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=31.5789473684211, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=30.3571428571429, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=30.4932735426009, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=28.9592760180996, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786784, Length=177, Percent_Identity=34.4632768361582, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=32.7433628318584, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787375, Length=218, Percent_Identity=32.5688073394495, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=31.1926605504587, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=29.1479820627803, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=25.1082251082251, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787229, Length=210, Percent_Identity=24.7619047619048, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=23.8297872340426, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24953; Mature: 24822

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKILVIDDEEDILILIKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGF
CCEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHH
TLCKEIREIVDCPILFLTAKTMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKRHIFVASDIIFNLSSKEIKIGDVKVPFTKGEYTICEYLARNSGQVFSKEQIYEAVYGY
HHHEEEEEHHEEEECCCCEEEECCEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
DGESDSSAITEHIKNIRAKLKVFDMSPIETVWGIGYKWV
CCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHCCCEECC
>Mature Secondary Structure 
SKILVIDDEEDILILIKNVLSKDNHLVTTVKEAKDIPLNDFVKFDLILLDVMMPDIDGF
CEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHH
TLCKEIREIVDCPILFLTAKTMESDIMFGLGIGGDDYITKPFGVGELRARVNAHLRREHR
HHHHHHHHHHCCCEEEEEHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKRHIFVASDIIFNLSSKEIKIGDVKVPFTKGEYTICEYLARNSGQVFSKEQIYEAVYGY
HHHEEEEEHHEEEECCCCEEEECCEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCC
DGESDSSAITEHIKNIRAKLKVFDMSPIETVWGIGYKWV
CCCCCHHHHHHHHHHHHHHEEEECCCCHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]