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The map label for this gene is 226948781

Identifier: 226948781

GI number: 226948781

Start: 1720361

End: 1721119

Strand: Direct

Name: 226948781

Synonym: CLM_1682

Alternate gene names: NA

Gene position: 1720361-1721119 (Clockwise)

Preceding gene: 226948780

Following gene: 226948782

Centisome position: 41.4

GC content: 31.75

Gene sequence:

>759_bases
ATGAAAATAGGAATAGATTGTGGTCATACATTAAGTGGTGCTGATTATGGAGCAGTAGGAATAAAAGCAGAATCTAATTT
AACTAGAGAGGTAGGAACAAAAGTTATAGCTAAGTTAAAAGCATTAGGTCATACAGTCATTAATTGTTATAAGGATAGTT
GTAGTAGTTTAAATGATAGTTTAAGCTATAGAACCAACACAGCTAATAATAACAATGTAGACTTATATGTATCTATTCAT
TTTAATTGTTATAACGGTAGTGCTTATGGTACAGAGGTTTTTACTTATGGAGGGAAATCTTTTACAGAAGCATCAAGAGT
ATTAAATAATATTTGTGCCTTAGGATATACAAATAGGGGGTTAAAAGATGGTTCTGGTCTTTATGTATTAAAGCATACAA
AAGCTAAAGCCATGCTAATAGAATGTTGTTTCTGCGATAATGCAGGAGATATGAACAGATATAATGCTGAAAATATGGCT
AATGCTATAGTTAAAGGACTAGTAGGTACTACAGTAGCGACACCAAATAAACCAAAGGAAGTGGTAAAAGTGCAAAAACC
AAAATATGATGAGACTATACCAACAGGAGAAAGTATATTTAAAATTCCAGGTACTACTGGATATATAGAGCAAGCCACAG
ACGGAAGATTGATAATACATAAGGATAGAGGAAATTATATAGCAATCGGTAAAGGCTTTATAGATTGCTATTGGAATGAT
AACAAAGGCAATGGCTGTAATAAAAGATTAAGTAATTAA

Upstream 100 bases:

>100_bases
TTATACAATGGTTGTTGCATTTTATTTTAGTAAGGATAGAAAAGAGCAAGACAAATAGTTTTGTTCTTTTTTTTATATTA
AATTTTAGGGAGGAATTTAT

Downstream 100 bases:

>100_bases
TTTTTAAAGGTACTTCTATAATGGAAGTACCTCTTTTTTTATTGGAAAAATTATTATAATTTATATAAATATTTCATAAA
AAGGTATTGATTTATTATAC

Product: bacteriophage endolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGTKVIAKLKALGHTVINCYKDSCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKSFTEASRVLNNICALGYTNRGLKDGSGLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGTTVATPNKPKEVVKVQKPKYDETIPTGESIFKIPGTTGYIEQATDGRLIIHKDRGNYIAIGKGFIDCYWND
NKGNGCNKRLSN

Sequences:

>Translated_252_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGTKVIAKLKALGHTVINCYKDSCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKSFTEASRVLNNICALGYTNRGLKDGSGLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGTTVATPNKPKEVVKVQKPKYDETIPTGESIFKIPGTTGYIEQATDGRLIIHKDRGNYIAIGKGFIDCYWND
NKGNGCNKRLSN
>Mature_252_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGTKVIAKLKALGHTVINCYKDSCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKSFTEASRVLNNICALGYTNRGLKDGSGLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGTTVATPNKPKEVVKVQKPKYDETIPTGESIFKIPGTTGYIEQATDGRLIIHKDRGNYIAIGKGFIDCYWND
NKGNGCNKRLSN

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To C.perfringens pIP404 ORF10 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 27359; Mature: 27359

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGTKVIAKLKALGHTVINCYKDSCSSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGTEVFTYGGKSFTEASRVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LKDGSGLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGTTVATPNKPKE
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCHH
VVKVQKPKYDETIPTGESIFKIPGTTGYIEQATDGRLIIHKDRGNYIAIGKGFIDCYWND
HHEECCCCCCCCCCCCCCEEECCCCCCCEEECCCCEEEEEECCCCEEEEECCEEEEEEEC
NKGNGCNKRLSN
CCCCCCCCCCCC
>Mature Secondary Structure
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGTKVIAKLKALGHTVINCYKDSCSSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGTEVFTYGGKSFTEASRVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
LKDGSGLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGTTVATPNKPKE
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEECCCCCCHH
VVKVQKPKYDETIPTGESIFKIPGTTGYIEQATDGRLIIHKDRGNYIAIGKGFIDCYWND
HHEECCCCCCCCCCCCCCEEECCCCCCCEEECCCCEEEEEECCCCEEEEECCEEEEEEEC
NKGNGCNKRLSN
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8052128; 11792842 [H]