| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is chiA [H]
Identifier: 226948358
GI number: 226948358
Start: 1292856
End: 1294439
Strand: Direct
Name: chiA [H]
Synonym: CLM_1240
Alternate gene names: 226948358
Gene position: 1292856-1294439 (Clockwise)
Preceding gene: 226948357
Following gene: 226948359
Centisome position: 31.11
GC content: 35.48
Gene sequence:
>1584_bases ATGAAATCACGAAAAATTACTGCAATTATTTTATCCGTATTGGTAGCTTCAGGAGCCACTGTATCAGTTTTTACAGGGAA AAATGTAGTACAAGCTAAAGAGTTAGAAATTAACAGTGTACAATCTAAAACAGCTACTAATATAGGAGTGGGACAAGAAT ATTGGGGATCTACTGTTTTCGCTCCTTATGTAGATGCTTGTTCATATCCTGTGTTTTCACTTACTGAATATGCAAAGGCA ACAGGAACTAAACATTTTATATTAGGATTTGTAGTTGATAAAAATGGACAACCTTCCTGGGGAACTTATTATGATATGGA AGAGGGGCCTACTGACTATCAAGGTGGAGGATTATTAAAAGAAATTAAAGCTTTAAGACAAATGGGCGGAGACGTAATGG TCTCTTTTGGTGGAGAAGCTAATACTCCGTTAGCAGCATCAATTAAGGATGTTAATAAATTAAAAGATGCATATAAGAAA ATAATCAAAGATTATGGATTAACTCATGTAGATTTTGACATTGAAGGAGCCTGGACAGCTGATGCCGCTTCTATAGAAAG ACGTTCACAAGCCATTGCATTACTTCAAAAGGAACTTAAAGCTGAAAATCATCCACTTGAAATATGGTATACATTACCTG TATTGCCTACAGGAATGACTAATGGTGTAGATGTAGTAAAAAATGCTGTAAAAAACGGTGTTCAGTTAAGTGGAGTAAAT GTAATGACTATGTGTTTTGGACAGAAGGCTCCAGATGGAACTATGGGGGATTTAGTTATACAATCAGCTAAAAGCGTTCA TAAACAATTAAAGGATATATACACACAAGCTAATATACAAAAGACTGATAAAGAAATTTGGAAAATGATAGGAGTAACTC CTATGAATGGTTTAGATTATAGTGGAAGTATACTTGATCAAAATGGAGCAAGTAAGGTCGCGAGCTTTGGTAAAACAGTA GGAATGGGACTTATAGGTATGTGGTCAGTAAATAGAGATTATCAAAATCCTAAGGGTTCAACAAATTACGTATCTATAAC GGATAGTTCTATATTACAAGAAAAATTTGAATTTGGTAAAATACTTTCAAAATATGATTCAAATGATGGTACAGAAATAA TAGAAAAGCCAGGTACAACTGATCCAGAAGATCCAGGCGATGGTGAAGATGGAGGAGAGACAGGGGAAATTGCTGCATAT AATCCTAATACAGAATATTGGGCAGGAGATAAAGTTAGCTATAAAGGAAAGGTTTATCAAGCTAAATGGTGGACAAAAGG GTTTGCACCAGATACGGCAGTACAAAATTCATGGGATACACCATGGGAACTAATAGGAGATTCAGATAATGATATAGAAA TACCAGAAAAACCAGGTACAACTGATCCAGTGGATCCAGAGGATGGTAAAGATGGGGAACAGACAGGAGAAATCGCTGCA TATACTCCTAATAAAGAATATTATGAAGGAGATAAAGTTACCTATAAAGGAAAGGTTTATCAAGCCAAATGGTGGACAAA AGGGTTTGCACCAGATACAGTAGTAAAAAATACATGGGATACACCTTGGGAGCTAATAGGATAA
Upstream 100 bases:
>100_bases ATCATTAGTGTGTTAATAGGAACTACGATACTTTCAACTGTTAGTTCCGTATCAACTTATGCAAAATCAAAAAAATATTA ATAATATGGGGGTAGATACA
Downstream 100 bases:
>100_bases GGTATAGTATTGATAAAATTAAATATATAAATTTTTCAAGAGTATTGGGTTAGAGGTATATTTCTCTCTAATCCAATACT TTATTTTAACCAATAAACAT
Product: putative glycosyl hydrolase
Products: NA
Alternate protein names: Chitinase; Lysozyme [H]
Number of amino acids: Translated: 527; Mature: 527
Protein sequence:
>527_residues MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG
Sequences:
>Translated_527_residues MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG >Mature_527_residues MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVFAPYVDACSYPVFSLTEYAKA TGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLKEIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKK IIKDYGLTHVDFDIEGAWTADAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDYSGSILDQNGASKVASFGKTV GMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGKILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAY NPNTEYWAGDKVSYKGKVYQAKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG
Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 5 chitin-binding type-3 domains [H]
Homologues:
Organism=Escherichia coli, GI1789736, Length=337, Percent_Identity=37.9821958456973, Blast_Score=207, Evalue=2e-54,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003610 - InterPro: IPR017853 - InterPro: IPR013781 [H]
Pfam domain/function: PF02839 CBM_5_12 [H]
EC number: =3.2.1.14; =3.2.1.17 [H]
Molecular weight: Translated: 57639; Mature: 57639
Theoretical pI: Translated: 4.61; Mature: 4.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVF CCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHCCHHHHHHHHHCCCCCCHHHHCCCCH APYVDACSYPVFSLTEYAKATGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHH EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKIIKDYGLTHVDFDIEGAWTA HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC DAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEECCCE VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDY EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC SGSILDQNGASKVASFGKTVGMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGK CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH ILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAYNPNTEYWAGDKVSYKGKVYQ HHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEEE AKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA EEECCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG ECCCCCCCCCCEEEEECEEEEEECCCCCCCCCCHHCCCCCCCHHHCC >Mature Secondary Structure MKSRKITAIILSVLVASGATVSVFTGKNVVQAKELEINSVQSKTATNIGVGQEYWGSTVF CCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHCCHHHHHHHHHCCCCCCHHHHCCCCH APYVDACSYPVFSLTEYAKATGTKHFILGFVVDKNGQPSWGTYYDMEEGPTDYQGGGLLK HHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHH EIKALRQMGGDVMVSFGGEANTPLAASIKDVNKLKDAYKKIIKDYGLTHVDFDIEGAWTA HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC DAASIERRSQAIALLQKELKAENHPLEIWYTLPVLPTGMTNGVDVVKNAVKNGVQLSGVN CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCEEECCCE VMTMCFGQKAPDGTMGDLVIQSAKSVHKQLKDIYTQANIQKTDKEIWKMIGVTPMNGLDY EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC SGSILDQNGASKVASFGKTVGMGLIGMWSVNRDYQNPKGSTNYVSITDSSILQEKFEFGK CCCEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHH ILSKYDSNDGTEIIEKPGTTDPEDPGDGEDGGETGEIAAYNPNTEYWAGDKVSYKGKVYQ HHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEEE AKWWTKGFAPDTAVQNSWDTPWELIGDSDNDIEIPEKPGTTDPVDPEDGKDGEQTGEIAA EEECCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE YTPNKEYYEGDKVTYKGKVYQAKWWTKGFAPDTVVKNTWDTPWELIG ECCCCCCCCCCEEEEECEEEEEECCCCCCCCCCHHCCCCCCCHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9278503; 2661540; 10419961; 10760150 [H]