Click here to switch to the map view.

The map label for this gene is gldA [H]

Identifier: 226948253

GI number: 226948253

Start: 1171197

End: 1172327

Strand: Reverse

Name: gldA [H]

Synonym: CLM_1132

Alternate gene names: 226948253

Gene position: 1172327-1171197 (Counterclockwise)

Preceding gene: 226948254

Following gene: 226948252

Centisome position: 28.21

GC content: 33.69

Gene sequence:

>1131_bases
ATGAGAAAAGCATTTATTTGTCCAACTAAATATGTTCAAGGTGAAGATGAAATATTAAACTTAGGATATTTTGTAAAGAG
TTTTGGGGAATCAGCTCTATTAATAGCTCACAAAGATGATATAGAACGTGTTAAAGATAAATTAGATTCAACTGCTAAAA
AATTTGGAATTACTTTTGTAGAAAGTGCATTCTGTGGAGAATGTTCAAGAGAAGAAGTTGCAAGATTACAAGAAATCGCA
AAAAATAATAAATGTGCATGCACTATAGGACTTGGCGGTGGTAAAGCTATAGATACAGCAAAATGCGTTGCTAAGGGTAA
TTCATTAATAATTGTTCCAACAATAGCAGCTACAGATGCTCCAACTAGTCACTCTGCTGTACTTTACACACCAGACGGTG
CATTTGATGATTACGCTTACTTCTGGCAAAGTCCAACTGTAATTTTAATAGATACTACAGTTATTGCAAATGCTCCAACA
AGATTCTTAGTTTCAGGAATGGGTGATGCATTATCAACATATTTCGAAGCAAGAGCAACTTCAAATTCTTTTTCAAATGT
TAATGCAGGTTTACCTTGCGGAGTTAGAGAAGGATTATGTGCACCAGCAAAAGGAACTAACACAGCATTAACACTTGCAA
AAGCTTGTTATAAAACATTACTAGAAGATGGTGTAAAAGCAAAAATAGCATCAGATTGTAATTTAGTAACACAAGCATTA
GAAAACATAATAGAAACAAATATTCTTTTATCAGGACTAGGTTTTGAAAGTGCTGGTTTAGCAGCTGCTCATGCAATCCA
TGATGGATTAACGATACTAGAAGGAACTCACAAATACTTCCATGGAGAAAAAGTTGCCTTTGGAACAATTGCCCAATTAG
TTCTTGAAAATGCACCAAAAGAAGAAATTGATGAAGTAATAAACTTCTGCCTAGAAATTGGACTTCCAGTTTGCTTAGAA
GATATTGGTGTAGACAGCATTACTGATGAAGAACTTATGGAAGTAGCTAAAAAATCTTGTATAGAAGAAGAATCAATACA
CTCTATGCCATTTAAGGTAACTCCAGAAGCAGTAGCAGCAGCAATAATAACAGCAGATACTTTAGGTAAAAAATATAAAT
CAAATAAATAG

Upstream 100 bases:

>100_bases
TTTTTTGTCATGTAAATCCAATGCATATTTATGTAAACGATATTACAATAAAGACAAGCTAAAAAATAAAAATATTTTTA
TAAATTAAGGGGAGATTATA

Downstream 100 bases:

>100_bases
AGGTGTAAACATGAAAAAGATAATAAATAGACCTGAAACTGTAGTTATGGAGATGTGTAATGGAATAGCTATGGCACATC
CTGAACTAGAGTTTGTTAGA

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRKAFICPTKYVQGEDEILNLGYFVKSFGESALLIAHKDDIERVKDKLDSTAKKFGITFVESAFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK

Sequences:

>Translated_376_residues
MRKAFICPTKYVQGEDEILNLGYFVKSFGESALLIAHKDDIERVKDKLDSTAKKFGITFVESAFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK
>Mature_376_residues
MRKAFICPTKYVQGEDEILNLGYFVKSFGESALLIAHKDDIERVKDKLDSTAKKFGITFVESAFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=374, Percent_Identity=43.8502673796791, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1786815, Length=305, Percent_Identity=30.1639344262295, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 40180; Mature: 40180

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAFICPTKYVQGEDEILNLGYFVKSFGESALLIAHKDDIERVKDKLDSTAKKFGITFV
CCCCEECCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCHHHH
ESAFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA
HHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEECCC
PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT
CCCCCEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCC
SNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK
HHHHHHHHHEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCH
EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCEECHHHHHH
AIITADTLGKKYKSNK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKAFICPTKYVQGEDEILNLGYFVKSFGESALLIAHKDDIERVKDKLDSTAKKFGITFV
CCCCEECCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCHHHH
ESAFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA
HHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEECCC
PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT
CCCCCEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCC
SNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK
HHHHHHHHHEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCH
EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCEECHHHHHH
AIITADTLGKKYKSNK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA