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The map label for this gene is dhaKL [H]

Identifier: 226948252

GI number: 226948252

Start: 1169426

End: 1171186

Strand: Reverse

Name: dhaKL [H]

Synonym: CLM_1131

Alternate gene names: 226948252

Gene position: 1171186-1169426 (Counterclockwise)

Preceding gene: 226948253

Following gene: 226948251

Centisome position: 28.19

GC content: 32.94

Gene sequence:

>1761_bases
ATGAAAAAGATAATAAATAGACCTGAAACTGTAGTTATGGAGATGTGTAATGGAATAGCTATGGCACATCCTGAACTAGA
GTTTGTTAGAAAATATAAAGTAATGAAAAAAAAGAATATAAACAAAAATAAAGTTAGCTTAATTAGTGGTGGTGGAAGTG
GTCACGAGCCAGCTCATGCCGGATTTATTGGAAAGGGAATGTTAGATGCAGCAGTATGCGGAGACATATTCGCTTCTCCC
TCTCAAATTCAAGTATATAAAGCTATAAAAGCTACAGCTAGCGAAAAAGGAACATTATTAATTATAAAAAACTATAGTGG
AGATATAATGAATTTTAAAAATGCTGCCCATTTAGCAAGTGAGGATGGTATTAAAGTTGACTATGTTAAAGTTGATGACG
ATATAGCTGTTGAAGATAGCTTATATACTGTAGGACGTAGAGGAGTTGCAGGTACTGTACTAGTTCATAAAATAGCAGGT
GCAGCTGCAGAATTAGGCTTTTCATTAGAAAAAGTTAAATCAATTGCTGAAAAAGCTGTTTCTAATGTTAGAAGCTTAGG
TTTTGCTTTCTCTTCTTGTACAGTTCCAGCTAAAAGAACTCCAACTTTTCAATTAGCAGAGGATGAAATGGAATTTGGTG
TTGGAATCCATGGCGAACCTGGAATAGTAAGAGAAAAGGTTGCTACAGCAGATGAATTAGCTAAAAAAATTGTTGATTCC
ATATTAAAAGACATGAAAATAGATGGATCAAATAATGAAGAAGTAGCCTTATTAATTAATGGTTTTGGTGCTACCCCTCT
ACAAGAATTATATCTATTTAATAATTCAGTTACTGCTGAGCTAGCAAAGAAAAATATAAAAATAAATAGAACATTTGTAG
GAAACTATATGACTAGTATAGATATGGAGGGCGCTTCAGTATCTATCATGAAATTAGATGATGAACTTAAAGAATTACTA
TCAAAAGAAAGTGATACTCCTGCATTTAAAGTTTCAGGACCAGTTGAATCAGTTGAATATATAAGCTTAGAAGATAATAA
TGATATTGAAAATGAAGTTAGTTTTGATTTGGAAACTTGTGATTGTCATAGCGAAATAAAAGATAAAAAAATTACTTTAG
ATAATATGATTTATATTATAGATAAAATGAGTGAAGTAATAATAGCAAATGAAGTTCACTTCTGTGAATTAGATTCTCAT
GCTGGTGATGGTGATTTTGGTATGAGTGTAGCAAAAGGTTTCAAACAATTAAAAAGAGAATGGAAACATCTTCTTAAAGA
AGATAATATGAATATTGGTAAATTCTTAAATGAATGCTCTTTAGTAATTATGGAACATTGTGGTGGAGCATCAGGCCCAA
TATGGGGATCAGCATTTAGAGCAGCAGGAAAACAAGTTGGAGATAAAAAAGAATTATCAGTTTCAGATTTTGCTGAAATG
ATGCAAGCAGCTGTAAAAGGAATTCAATCTACAGGAGAACGTTCTTTTGGAAGAGGAGCAGTAGTTGGAGATAAAACTTT
AATTGACGCATTAGTTCCTTGTGCTGATTTGTGGAGTGAAAGTGCTAATAACAATACTTCTATACATGAAGCCTTCCAAA
AAGGTGCCGCTGCAGCAGTTAAAGGTGCAAAAATGACTGAAGAAATAGTAGCTCGTATGGGTAGAGCAGGTACAGTTGGT
GAAAGAAGCATTGGTTACCCAGATGCAGGCGCTTATGGATTAGGAGTTATATTTACAGAAATATCAAATTCATTAAAATA
A

Upstream 100 bases:

>100_bases
TCTATGCCATTTAAGGTAACTCCAGAAGCAGTAGCAGCAGCAATAATAACAGCAGATACTTTAGGTAAAAAATATAAATC
AAATAAATAGAGGTGTAAAC

Downstream 100 bases:

>100_bases
GCCTTCTAATATTATAATAGCTCATACAATATTATAATTTAAAATTTAACCTAAAAACTCCAGTAAACACCTTATTTTTT
ACTGGAGTTTTTATTTTTAC

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKKIINRPETVVMEMCNGIAMAHPELEFVRKYKVMKKKNINKNKVSLISGGGSGHEPAHAGFIGKGMLDAAVCGDIFASP
SQIQVYKAIKATASEKGTLLIIKNYSGDIMNFKNAAHLASEDGIKVDYVKVDDDIAVEDSLYTVGRRGVAGTVLVHKIAG
AAAELGFSLEKVKSIAEKAVSNVRSLGFAFSSCTVPAKRTPTFQLAEDEMEFGVGIHGEPGIVREKVATADELAKKIVDS
ILKDMKIDGSNNEEVALLINGFGATPLQELYLFNNSVTAELAKKNIKINRTFVGNYMTSIDMEGASVSIMKLDDELKELL
SKESDTPAFKVSGPVESVEYISLEDNNDIENEVSFDLETCDCHSEIKDKKITLDNMIYIIDKMSEVIIANEVHFCELDSH
AGDGDFGMSVAKGFKQLKREWKHLLKEDNMNIGKFLNECSLVIMEHCGGASGPIWGSAFRAAGKQVGDKKELSVSDFAEM
MQAAVKGIQSTGERSFGRGAVVGDKTLIDALVPCADLWSESANNNTSIHEAFQKGAAAAVKGAKMTEEIVARMGRAGTVG
ERSIGYPDAGAYGLGVIFTEISNSLK

Sequences:

>Translated_586_residues
MKKIINRPETVVMEMCNGIAMAHPELEFVRKYKVMKKKNINKNKVSLISGGGSGHEPAHAGFIGKGMLDAAVCGDIFASP
SQIQVYKAIKATASEKGTLLIIKNYSGDIMNFKNAAHLASEDGIKVDYVKVDDDIAVEDSLYTVGRRGVAGTVLVHKIAG
AAAELGFSLEKVKSIAEKAVSNVRSLGFAFSSCTVPAKRTPTFQLAEDEMEFGVGIHGEPGIVREKVATADELAKKIVDS
ILKDMKIDGSNNEEVALLINGFGATPLQELYLFNNSVTAELAKKNIKINRTFVGNYMTSIDMEGASVSIMKLDDELKELL
SKESDTPAFKVSGPVESVEYISLEDNNDIENEVSFDLETCDCHSEIKDKKITLDNMIYIIDKMSEVIIANEVHFCELDSH
AGDGDFGMSVAKGFKQLKREWKHLLKEDNMNIGKFLNECSLVIMEHCGGASGPIWGSAFRAAGKQVGDKKELSVSDFAEM
MQAAVKGIQSTGERSFGRGAVVGDKTLIDALVPCADLWSESANNNTSIHEAFQKGAAAAVKGAKMTEEIVARMGRAGTVG
ERSIGYPDAGAYGLGVIFTEISNSLK
>Mature_586_residues
MKKIINRPETVVMEMCNGIAMAHPELEFVRKYKVMKKKNINKNKVSLISGGGSGHEPAHAGFIGKGMLDAAVCGDIFASP
SQIQVYKAIKATASEKGTLLIIKNYSGDIMNFKNAAHLASEDGIKVDYVKVDDDIAVEDSLYTVGRRGVAGTVLVHKIAG
AAAELGFSLEKVKSIAEKAVSNVRSLGFAFSSCTVPAKRTPTFQLAEDEMEFGVGIHGEPGIVREKVATADELAKKIVDS
ILKDMKIDGSNNEEVALLINGFGATPLQELYLFNNSVTAELAKKNIKINRTFVGNYMTSIDMEGASVSIMKLDDELKELL
SKESDTPAFKVSGPVESVEYISLEDNNDIENEVSFDLETCDCHSEIKDKKITLDNMIYIIDKMSEVIIANEVHFCELDSH
AGDGDFGMSVAKGFKQLKREWKHLLKEDNMNIGKFLNECSLVIMEHCGGASGPIWGSAFRAAGKQVGDKKELSVSDFAEM
MQAAVKGIQSTGERSFGRGAVVGDKTLIDALVPCADLWSESANNNTSIHEAFQKGAAAAVKGAKMTEEIVARMGRAGTVG
ERSIGYPDAGAYGLGVIFTEISNSLK

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=578, Percent_Identity=31.3148788927336, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=43.1818181818182, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787449, Length=179, Percent_Identity=30.7262569832402, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565018, Length=588, Percent_Identity=30.952380952381, Blast_Score=276, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321055, Length=599, Percent_Identity=32.0534223706177, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323570, Length=593, Percent_Identity=33.0522765598651, Blast_Score=233, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 63270; Mature: 63270

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIINRPETVVMEMCNGIAMAHPELEFVRKYKVMKKKNINKNKVSLISGGGSGHEPAHA
CCCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
GFIGKGMLDAAVCGDIFASPSQIQVYKAIKATASEKGTLLIIKNYSGDIMNFKNAAHLAS
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCC
EDGIKVDYVKVDDDIAVEDSLYTVGRRGVAGTVLVHKIAGAAAELGFSLEKVKSIAEKAV
CCCCEEEEEEECCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SNVRSLGFAFSSCTVPAKRTPTFQLAEDEMEFGVGIHGEPGIVREKVATADELAKKIVDS
HHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILKDMKIDGSNNEEVALLINGFGATPLQELYLFNNSVTAELAKKNIKINRTFVGNYMTSI
HHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCEEEEEEECCHHEEE
DMEGASVSIMKLDDELKELLSKESDTPAFKVSGPVESVEYISLEDNNDIENEVSFDLETC
ECCCCEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHEECEECC
DCHSEIKDKKITLDNMIYIIDKMSEVIIANEVHFCELDSHAGDGDFGMSVAKGFKQLKRE
CCHHHHCCCEEEHHHHHHHHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
WKHLLKEDNMNIGKFLNECSLVIMEHCGGASGPIWGSAFRAAGKQVGDKKELSVSDFAEM
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MQAAVKGIQSTGERSFGRGAVVGDKTLIDALVPCADLWSESANNNTSIHEAFQKGAAAAV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHH
KGAKMTEEIVARMGRAGTVGERSIGYPDAGAYGLGVIFTEISNSLK
HCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIINRPETVVMEMCNGIAMAHPELEFVRKYKVMKKKNINKNKVSLISGGGSGHEPAHA
CCCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
GFIGKGMLDAAVCGDIFASPSQIQVYKAIKATASEKGTLLIIKNYSGDIMNFKNAAHLAS
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCC
EDGIKVDYVKVDDDIAVEDSLYTVGRRGVAGTVLVHKIAGAAAELGFSLEKVKSIAEKAV
CCCCEEEEEEECCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SNVRSLGFAFSSCTVPAKRTPTFQLAEDEMEFGVGIHGEPGIVREKVATADELAKKIVDS
HHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILKDMKIDGSNNEEVALLINGFGATPLQELYLFNNSVTAELAKKNIKINRTFVGNYMTSI
HHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCEEEEEEECCHHEEE
DMEGASVSIMKLDDELKELLSKESDTPAFKVSGPVESVEYISLEDNNDIENEVSFDLETC
ECCCCEEEEEECCHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCHHEECEECC
DCHSEIKDKKITLDNMIYIIDKMSEVIIANEVHFCELDSHAGDGDFGMSVAKGFKQLKRE
CCHHHHCCCEEEHHHHHHHHHHHHHHHEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
WKHLLKEDNMNIGKFLNECSLVIMEHCGGASGPIWGSAFRAAGKQVGDKKELSVSDFAEM
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
MQAAVKGIQSTGERSFGRGAVVGDKTLIDALVPCADLWSESANNNTSIHEAFQKGAAAAV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHH
KGAKMTEEIVARMGRAGTVGERSIGYPDAGAYGLGVIFTEISNSLK
HCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]