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The map label for this gene is cobD [H]

Identifier: 226948187

GI number: 226948187

Start: 1112518

End: 1113489

Strand: Direct

Name: cobD [H]

Synonym: CLM_1062

Alternate gene names: 226948187

Gene position: 1112518-1113489 (Clockwise)

Preceding gene: 226948186

Following gene: 226948188

Centisome position: 26.77

GC content: 29.12

Gene sequence:

>972_bases
ATGTATCTAAATATAACTACAAATTTTATAGATATAATTATAGCGGTTTTTATAGATTGGATTATTGGAGATCCTTATTG
GTTTCCCCATCCAGTTATATACATAGGAAAGCTTATTAGTTTTTTAGAAAAAAAATTTAGAACTAAAGTAAAAAATGAAG
AAAATTTAAAAATTTATGGTGGAATAATTGTACTGATAGTTTGTCTTGCTAGTTTTTTAATTCCCTTTATGCTTTTACAA
GCTTTTAAATTTAATTTTTATCTTTATCATGGGCTAAATATTTTAATAATATGGACAACTTTAGCGGCTAAATGTCTCCA
TGGGGAAGCTATAAAAATATATCATGCTTTATATAAAGAAAATATAGAGGAAGCAAGGTTAAAACTTTCTTACATAGTAG
GAAGAGAAACTAAAGATTTAATTGGAAATGAAATAATGAGGGCAGATATAGAAACTGTAGCAGAAAATGCATCAGATGGG
GTAATAGCACCACTTTTTTATGCAATAATTGGTGGAGCACCTTTAGCTATGATGTATAAAGGGATAAATACTATGGATTC
TATGCTTGGATATTTAAATGATGAATATAGATATATAGGATTTTTCCCAGCTAAGGTAGATGATATTTTTAATTTTGTAC
CAGCAAGATTAACAGGAATATTAATGTGTATATCGGCACCGTTAGTAGGTGGGAATCCATTTAAAAGTTTTAAAATAATG
ATAAGAGATAGAAAAAATCATAAAAGTCCTAATTGTGCCTATCCTGAAGGAGCTACAGCAGCAGCCTTAAAGATACAATT
GGGAGGAACGAATGTATATTTTGGCCAAGTGGTAGAAAAACCTACTATAGGAGATAAGATAAAGGAACTGGCTCCAATAC
ATATAAAAGAAAGTATAAAACTTATGTATGCTAGTGAAGCGTTAATGATAGTAATGGTAGGCGTACTTCTTAAACTTTTA
TTTGAAATATAA

Upstream 100 bases:

>100_bases
TATGAACATATGAGAGAAAAAGAGTTAGATAAGCTTGCGGATATAGTAAGGCATAGCTTAGATATGGAAAAAATTTATTC
TATTATAGGGATGAAATAAG

Downstream 100 bases:

>100_bases
AGAATAGTTTTTTTATCTTTAAAATTAATGATAAGTGATGATTAATTTAACTATAGAGATAGTTCTTTAATTGCAGTAAT
TAAGTGAAAGTGAAAATCAG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGKLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYLYHGLNILIIWTTLAAKCLHGEAIKIYHALYKENIEEARLKLSYIVGRETKDLIGNEIMRADIETVAENASDG
VIAPLFYAIIGGAPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPLVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEALMIVMVGVLLKLL
FEI

Sequences:

>Translated_323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGKLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYLYHGLNILIIWTTLAAKCLHGEAIKIYHALYKENIEEARLKLSYIVGRETKDLIGNEIMRADIETVAENASDG
VIAPLFYAIIGGAPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPLVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEALMIVMVGVLLKLL
FEI
>Mature_323_residues
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGKLISFLEKKFRTKVKNEENLKIYGGIIVLIVCLASFLIPFMLLQ
AFKFNFYLYHGLNILIIWTTLAAKCLHGEAIKIYHALYKENIEEARLKLSYIVGRETKDLIGNEIMRADIETVAENASDG
VIAPLFYAIIGGAPLAMMYKGINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPLVGGNPFKSFKIM
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIKLMYASEALMIVMVGVLLKLL
FEI

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 36442; Mature: 36442

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGKLISFLEKKFRTKVKNEENLKIYG
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
GIIVLIVCLASFLIPFMLLQAFKFNFYLYHGLNILIIWTTLAAKCLHGEAIKIYHALYKE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHCCCHHHHHHHHHHH
NIEEARLKLSYIVGRETKDLIGNEIMRADIETVAENASDGVIAPLFYAIIGGAPLAMMYK
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
GINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPLVGGNPFKSFKIM
CCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEE
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIK
EEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCCCHHHHHHHCCCHHHHHHH
LMYASEALMIVMVGVLLKLLFEI
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYLNITTNFIDIIIAVFIDWIIGDPYWFPHPVIYIGKLISFLEKKFRTKVKNEENLKIYG
CEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
GIIVLIVCLASFLIPFMLLQAFKFNFYLYHGLNILIIWTTLAAKCLHGEAIKIYHALYKE
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEHHHHHHHHHHCCCHHHHHHHHHHH
NIEEARLKLSYIVGRETKDLIGNEIMRADIETVAENASDGVIAPLFYAIIGGAPLAMMYK
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
GINTMDSMLGYLNDEYRYIGFFPAKVDDIFNFVPARLTGILMCISAPLVGGNPFKSFKIM
CCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEE
IRDRKNHKSPNCAYPEGATAAALKIQLGGTNVYFGQVVEKPTIGDKIKELAPIHIKESIK
EEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCCCHHHHHHHCCCHHHHHHH
LMYASEALMIVMVGVLLKLLFEI
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]