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The map label for this gene is ascB [H]

Identifier: 226948151

GI number: 226948151

Start: 1077200

End: 1078621

Strand: Direct

Name: ascB [H]

Synonym: CLM_1021

Alternate gene names: 226948151

Gene position: 1077200-1078621 (Clockwise)

Preceding gene: 226948150

Following gene: 226948152

Centisome position: 25.92

GC content: 32.35

Gene sequence:

>1422_bases
ATGAGTGGATTAGCAAAGGACTTTTTATGGGGTGGAGCTACAGCAGCTAACCAGTGCGAAGGTGGATATTTAGAAGGAAA
TAGAGGATTAGGAACTGTGGACGTTATTCCAGCAGGAGTAAATCGTAGAGCTGTTATGCAAGGAAAGATGCATTATAATA
ATCTACAAGAGGATTCTTATTTCCCATCACATGAAGCAATAGATATGTATCATAGCTACAAGGAGGATATAGCATTATTT
GCAGAAATGGGATTTAAATGTTATCGTTTTTCACTTTCATGGTCAAGAATTTTCCCAACAGGAAAGGAACAAGAACCAAA
TGAAGAAGGCTTAGCATTTTATGAAAAATTTATTGATGAGCTATTAAAATATAATATTGAACCAGTAGTTACCATATGCC
ATTTTGATGTACCTCTTGTATTGGAGCAGGAATATGGTTCATGGAGAAGTAGAAAATTAATAGATGCATATCTTAGATAT
TGTGAAGCAATATTTAAAAGATTTAATGGGAAAGTAAAGTATTGGATAACTTTTAATGAAATAAATATGCTTATGCATTT
ACCCTTTATGGGAGCTGGAATAATGTTTAAGGAAGGGGAAATTGAGCTAGAAGTAAAATATCAAGCAGCTCATAATGAAT
TAGTGGCATCAGCCTTAGCTACAAAATTAGCAAAGGAAATTAATAAAGATAATAAAGTAGGATGTATGTTAGCGGCAGGG
CAATTCTATCCATATACTTGTAAGCCAGAAGATGTTCTAGAAGCTATGGAGAAAGATAAAAATAATTATTTCTTCATAGA
TGTACAATCTAGGGGAGAATACCCGAGCTATGCTAAAAAATTCTTAGAGAGAAACAATATAAAAATAGAAATGCAGTCAG
AAGATGAGAAGATTTTAAGGGAGAATACTGTTGATTTTATTGCATTTAGTTACTATACTTCAAGACTTGCAAGTAGTGAC
CCGAATGCAGGAGAAAAATCAGCTGGTAATGTAATAACATCATTAAAAAATCCACATTTAAAATCTAGTAAATGGGGATG
GCAAATAGATCCAATAGGCCTTAGAATTACAATGAATTCACTTTATGATCGTTATCAAAAACCGCTATTCATTGTGGAAA
ATGGCTTAGGTGCTGTTGATATTTTAAATGAGGATGGGACTGTAGATGATGATTATAGAATTGCTTATCTTGAAACTCAT
ATAAAAAATATGATTGATGCAGTTGAATTAGATGGTGTTGAACTATTAGGATATACAACTTGGGGATGCATTGATTTAGT
TAGTGCATCAACAGGGGAAATGAAAAAGCGATATGGTTTTATATATGTAGATAAGGATAATGATGGTCATGGAACTTTAA
AGAGATACAAGAAAAAATCTTTCTATTGGTACAAAAATGTTATTAAAAATAATGGACTATAA

Upstream 100 bases:

>100_bases
AGCTTATTCATATGCTTATTACATATATTTTTTATATTTACTATATTAAAATTAAAACTATTTTGAGGCATCACCTTCTT
TAATGAAAGGAAGGAAAAAT

Downstream 100 bases:

>100_bases
ATAATTGAAAAAATATATAATATATAAATTTGTAATAGCAATAGGCCAAAAATAAGGGAAGATTAGGAATGTAAAATATT
TTAGGATTTACTGAGGCATA

Product: glycosyl hydrolase, family 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHSYKEDIALF
AEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGEIELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Sequences:

>Translated_473_residues
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHSYKEDIALF
AEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGEIELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
>Mature_472_residues
SGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHSYKEDIALFA
EMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYC
EAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGEIELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAGQ
FYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSDP
NAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHI
KNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=28.8343558282209, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI13273313, Length=485, Percent_Identity=29.0721649484536, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI110681710, Length=497, Percent_Identity=28.9738430583501, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=27.9835390946502, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI28376633, Length=483, Percent_Identity=25.0517598343685, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1789070, Length=472, Percent_Identity=60.1694915254237, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI2367174, Length=480, Percent_Identity=51.875, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=51.3742071881607, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17552856, Length=496, Percent_Identity=30.4435483870968, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17539390, Length=496, Percent_Identity=28.2258064516129, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21356577, Length=470, Percent_Identity=25.7446808510638, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54312; Mature: 54181

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHSYKEDIALFAEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWITFNE
HHHCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
INMLMHLPFMGAGIMFKEGEIELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
EEEEEECCCCCCCEEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHSYKEDIALFAEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWITFNE
HHHCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
INMLMHLPFMGAGIMFKEGEIELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
EEEEEECCCCCCCEEEECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]