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The map label for this gene is brnQ [H]

Identifier: 226948091

GI number: 226948091

Start: 1013871

End: 1015172

Strand: Direct

Name: brnQ [H]

Synonym: CLM_0954

Alternate gene names: 226948091

Gene position: 1013871-1015172 (Clockwise)

Preceding gene: 226948090

Following gene: 226948092

Centisome position: 24.4

GC content: 32.41

Gene sequence:

>1302_bases
ATGAAGAAAACATCAGCAAAAGATTTCATAGTTATTGGTTTTGCACTTTTCGCAATGTTTTTTGGGGCAGGCAACTTAAT
CTTTCCACCTTTTATGGGAAAATTAGTAGGGGATCAGGCACCAGCAGCAATAATAGGTTTTTTAATTACCGGAGTAGGTT
TACCATTAACTGGTATAATAGCCTGTGCTAAAATAAATGGGACATTTTCAGATATATCAGGAAGAGTAGGGAAGATATTT
GCTATTATATCTACAACAGCATTAATATTAGCTATAGGACCTATGCTTGCTATACCAAGAACCGCAGCTACCACTTATGA
ATTAGCAATACATCCAATATTTCCGGGGGTAGCTCCAGTAGTAGCAGTTATAATTTATTTTCTTGTTTGTTTAGCATTTG
TATTAAGACCATCAGGAATAGTAGATAGTATAGGTAAAGTTTTAACACCAGCTTTATTAGTTATGTTAGCAATTATAATA
ATTAAAGGATTAGTTTCTCCTCTAGGACCTACTATATCAACTGGATTTAAAGGGGCTTTTTCAAAATCTTTACTTGAAGG
ATATCAAACAATGGATGCTATGGCATCAGTAATATTTGCATCCATAATAATAACAGCTGTAAGAGCAAAGGGATATACAG
AGAAAAAAGATATTGTAAGTCTTACTATAAAATCAGGTATTGTAGCTGCAGTAGGGTTGGCTTTTGTTTATGGAGGACTT
ATGATTCTAGGAAGTCATACTTCACAAATAATTCCTGGAGAAATAGGAAGATCAGCTCTAGTTGTTGAGATAGTTAAAAG
ACTTTTAGGTAATGCAGGTACAGTTATATTAGGATTAGCTGTTGGACTTGCATGTCTTACAACAGCAATCGGACTTATAT
CTACAGGAGCAGAATATTTCAGTAGTTTAACTAAGGGTAAAGTACCCTATAATGTTTTCGCAATTATTATAAGTGCAGTA
AGTGCATTACTTGGAACAGGTGGAGTTGATAAAATAGTTAAATTCTCAGTTCCAATATTGCAAGTTTTATATCCAATAGT
AATAGTTTTAATAGCAATAACTTTAGCAGGAAAACTTGTTAAAAATAATACTGTTGTAAGAATTACTGTTTACACTACAT
TAGTAGTAAGTTTCATAGATACAATAAATATATTAACAAATGGCAATGTAGCTTTTATAAAAAGTTTATTAGGAATTATA
CCACTTTCTAATGCTGGTTTTTCATGGTTAATTCCAGCAGTCATAGCATTTATAATAGGTACAGTAGCCTTTGGAAAGAA
ACAAGATATAGCAGATGCTTAA

Upstream 100 bases:

>100_bases
ATAAAATTAAAATTTCAGAATTCATTAAATTTTGAAAATAAAAATAATATGATATAATAACTAATAAAATTATATAAAAT
TTGAGTTAAGGGGGACTAAA

Downstream 100 bases:

>100_bases
TTTAATATAATAAAAAGCACCTTATTGCAAAACAATAAGGTGTTTTTTTATTTTTCTATTAAAGTTTCAGAATTTAATCA
ATTTTCCCAATAATCTTAAT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDQAPAAIIGFLITGVGLPLTGIIACAKINGTFSDISGRVGKIF
AIISTTALILAIGPMLAIPRTAATTYELAIHPIFPGVAPVVAVIIYFLVCLAFVLRPSGIVDSIGKVLTPALLVMLAIII
IKGLVSPLGPTISTGFKGAFSKSLLEGYQTMDAMASVIFASIIITAVRAKGYTEKKDIVSLTIKSGIVAAVGLAFVYGGL
MILGSHTSQIIPGEIGRSALVVEIVKRLLGNAGTVILGLAVGLACLTTAIGLISTGAEYFSSLTKGKVPYNVFAIIISAV
SALLGTGGVDKIVKFSVPILQVLYPIVIVLIAITLAGKLVKNNTVVRITVYTTLVVSFIDTINILTNGNVAFIKSLLGII
PLSNAGFSWLIPAVIAFIIGTVAFGKKQDIADA

Sequences:

>Translated_433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDQAPAAIIGFLITGVGLPLTGIIACAKINGTFSDISGRVGKIF
AIISTTALILAIGPMLAIPRTAATTYELAIHPIFPGVAPVVAVIIYFLVCLAFVLRPSGIVDSIGKVLTPALLVMLAIII
IKGLVSPLGPTISTGFKGAFSKSLLEGYQTMDAMASVIFASIIITAVRAKGYTEKKDIVSLTIKSGIVAAVGLAFVYGGL
MILGSHTSQIIPGEIGRSALVVEIVKRLLGNAGTVILGLAVGLACLTTAIGLISTGAEYFSSLTKGKVPYNVFAIIISAV
SALLGTGGVDKIVKFSVPILQVLYPIVIVLIAITLAGKLVKNNTVVRITVYTTLVVSFIDTINILTNGNVAFIKSLLGII
PLSNAGFSWLIPAVIAFIIGTVAFGKKQDIADA
>Mature_433_residues
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDQAPAAIIGFLITGVGLPLTGIIACAKINGTFSDISGRVGKIF
AIISTTALILAIGPMLAIPRTAATTYELAIHPIFPGVAPVVAVIIYFLVCLAFVLRPSGIVDSIGKVLTPALLVMLAIII
IKGLVSPLGPTISTGFKGAFSKSLLEGYQTMDAMASVIFASIIITAVRAKGYTEKKDIVSLTIKSGIVAAVGLAFVYGGL
MILGSHTSQIIPGEIGRSALVVEIVKRLLGNAGTVILGLAVGLACLTTAIGLISTGAEYFSSLTKGKVPYNVFAIIISAV
SALLGTGGVDKIVKFSVPILQVLYPIVIVLIAITLAGKLVKNNTVVRITVYTTLVVSFIDTINILTNGNVAFIKSLLGII
PLSNAGFSWLIPAVIAFIIGTVAFGKKQDIADA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=421, Percent_Identity=31.1163895486936, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 44883; Mature: 44883

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDQAPAAIIGFLITGVGLPLTGII
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDISGRVGKIFAIISTTALILAIGPMLAIPRTAATTYELAIHPIFPGVAPV
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
VAVIIYFLVCLAFVLRPSGIVDSIGKVLTPALLVMLAIIIIKGLVSPLGPTISTGFKGAF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHH
SKSLLEGYQTMDAMASVIFASIIITAVRAKGYTEKKDIVSLTIKSGIVAAVGLAFVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MILGSHTSQIIPGEIGRSALVVEIVKRLLGNAGTVILGLAVGLACLTTAIGLISTGAEYF
HEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
SSLTKGKVPYNVFAIIISAVSALLGTGGVDKIVKFSVPILQVLYPIVIVLIAITLAGKLV
HHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNTVVRITVYTTLVVSFIDTINILTNGNVAFIKSLLGIIPLSNAGFSWLIPAVIAFIIG
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TVAFGKKQDIADA
HHHCCCCCCCCCC
>Mature Secondary Structure
MKKTSAKDFIVIGFALFAMFFGAGNLIFPPFMGKLVGDQAPAAIIGFLITGVGLPLTGII
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
ACAKINGTFSDISGRVGKIFAIISTTALILAIGPMLAIPRTAATTYELAIHPIFPGVAPV
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHH
VAVIIYFLVCLAFVLRPSGIVDSIGKVLTPALLVMLAIIIIKGLVSPLGPTISTGFKGAF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHH
SKSLLEGYQTMDAMASVIFASIIITAVRAKGYTEKKDIVSLTIKSGIVAAVGLAFVYGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MILGSHTSQIIPGEIGRSALVVEIVKRLLGNAGTVILGLAVGLACLTTAIGLISTGAEYF
HEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
SSLTKGKVPYNVFAIIISAVSALLGTGGVDKIVKFSVPILQVLYPIVIVLIAITLAGKLV
HHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNTVVRITVYTTLVVSFIDTINILTNGNVAFIKSLLGIIPLSNAGFSWLIPAVIAFIIG
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TVAFGKKQDIADA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]