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The map label for this gene is sbcD [H]

Identifier: 226947708

GI number: 226947708

Start: 598681

End: 599910

Strand: Direct

Name: sbcD [H]

Synonym: CLM_0555

Alternate gene names: 226947708

Gene position: 598681-599910 (Clockwise)

Preceding gene: 226947707

Following gene: 226947709

Centisome position: 14.41

GC content: 31.22

Gene sequence:

>1230_bases
ATGAGAATATTGCATACCTCCGATTGGCACTTGGGAAAGAACCTTGAAGGCTTTAGTAGAATGGATGAACAGGAAAGATT
TATAGAGGATTTTATTAACATTGTGGATAAAAATAATATAGATATGGTGATAATAGCTGGAGATATATATGATAACGGGA
ATCCACCGGCTAGAGCAGAAAAAATGTTTTATGATTGTATAAAAAAAGTAAGCAATAAAGGAGAAAGATTAGTTTTAATT
ATAGCAGGTAATCACGATAATCCAGAAAGATTAGTTGCTGCAAGTTCTTTAGCCTATGATGAGGGAATAATACTTATGGG
GACCCCTAAGACTATAGTTCCAAAGGGAAAGTATGGGAATTTTAAGGTAATAAATTCAGATTTAGCCTGTTTAGAAATAG
AGGTAAAGGAAGAAAAGGCAGTGATTATATCTTTACCTTATCCTAGTGAGAAAAGATTAAATGAAATACTGTCTAAAGAA
ATGGATAATGATGAGGATAGAAGAAAAACTTATTCAGAAAGGATAGGAGATATATTTTCTAATTTACAGGAAAAATATTA
TAAAGATAACACTGTTAATTTAGCTATTTCTCATATATTTGTAAATGGATCAGAGGAAACAGATTCTGAAAGGCCAATAC
AACTTGGAGGGAGTCTAGCGGTAGATGTAGACAAGTTACCTAAAAAAGCACAATATATAGGCCTTGGACATCTTCATAAA
CCACAAAAGGTAAGGGGGCTAGATAATGCCTATTATTCTGGTTCGCCACTGCCTTATAGTAAAAGTGAAAGAGGATATAG
TAAAGGATGCAAAATAATAGAGGCACATCCAGGGGAAAAAGCTATTATTGAGGAGGTTTATTTTAATAACTATAAACCTA
TAGAGGTATGGCATTGCAACGGTATAAAAGAGGCTATAAATAAATGTGAAGAAAATAAAGAAAAGGATATGTGGCTATAT
TTACATATAAAAACAAAGGAATATATAAGTACAGAATATATAAAAACTTTGAAAGAAATAAAAAAAGATATAGTAGAGAT
AGTACCAGAGATAGAGGAAAAAAGAGAAGAAACTTTTAATGGAAATATAAAAGAAAAATCTATGGCAGAATTATTTAAAT
CCTTCTATTTAAATCAAAGGGGAATAGAGGCTAGTGAAGAATTAATGGATTTATTTTTAAGTATAGCTCAAGAGGAGGAT
GAACATGAAACCGAAGAAACTTGTGATTAA

Upstream 100 bases:

>100_bases
GATAACAATATAATTTATTTTTATATAGATTGTGAGTAGAATAAGCAAAATAATTTAATAAGATGGAAAGTTAACTATAT
ACAAAAGGGAGATGAAGATC

Downstream 100 bases:

>100_bases
AGGGCTAAACAGTTTTATAGAAAAACAAGAAATAGATTTTGAAACTTTAGTTAATAGAGGTTTGTTTGGTATATTTGGTC
CTACAGGCAGTGGAAAATCC

Product: exonuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGLDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGEKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEYIKTLKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EHETEETCD

Sequences:

>Translated_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGLDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGEKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEYIKTLKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EHETEETCD
>Mature_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKVSNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
PQKVRGLDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGEKAIIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEYIKTLKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EHETEETCD

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=393, Percent_Identity=26.7175572519084, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 47037; Mature: 47037

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHH
KMFYDCIKKVSNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEEEHHHCCCHHHEECCCCCCC
PQKVRGLDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGEKAIIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEYIKTLKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEHETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHCCC
>Mature Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHH
KMFYDCIKKVSNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIEVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLAVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEEEHHHCCCHHHEECCCCCCC
PQKVRGLDNAYYSGSPLPYSKSERGYSKGCKIIEAHPGEKAIIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEYIKTLKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEHETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]