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The map label for this gene is braC [H]

Identifier: 226947689

GI number: 226947689

Start: 582920

End: 584062

Strand: Direct

Name: braC [H]

Synonym: CLM_0536

Alternate gene names: 226947689

Gene position: 582920-584062 (Clockwise)

Preceding gene: 226947688

Following gene: 226947690

Centisome position: 14.03

GC content: 31.76

Gene sequence:

>1143_bases
GTGAAATTTGCAAAATTTAAGAAAAAATCTTTAATTGCATTATCTAGCTTACTAGTATTAGGTAGTTTAGTTGGTTGCCA
ATCAAATCCAGGTGAGAAAGAGGAAACTATAAAGGTTGGACTAGTTGCACCATTATCTGGAGCTATGGCACAGGATGGTA
AAAGTATATTAAATGCAGCTCAATTAGCAGTTGATGAAGTTAATAAAGAAGGCGGAATTAACAAGAAAAAAATAGAATTA
GTTTACGAAGATGATAAGGGAGAGCCAAAGGAGGCTGCATCCATTGCAAATAAATATTCATCAGATAAAGATATTATGGC
GGTTATGGGAAGCTTTTCTGCACCATGTACTTTAGCTGGAATACCTATTTATAATAAAGCTAAAATTCCAATGATGGGAC
CTTGTGGTTCAGCACCAGCTTTAAGTGGTAGCAGTGAATATTATAGAAAAGTAACTCCATCAGATTTAACAACAGGAAAA
GAACTATCAAAATGGATGTTAAAAGATATGAATTATAAAAAAGTTGCTATTATATATGTAAACAACGATTATGGTAAAGG
CGTTGCGGAAAGTGTAGATAATTTTTATAAAGAAAATGGAGGACAAGTTGTAGCTAAAGAAAGTTACATGCCTAAAACTC
AAGACTTTGGAAGTATAATAACAAAAGTAAAAAGCAAAAATCCAGATATAATCATAATGGGGTCTTTTTATGGTGATGCA
GGTGCCTTTCTTAAACAAGCTCATAATGTAGGATACAATCCTAAAGTAGCAGGACCTACTCCATTACTTTCAAAATCATT
AGTAGATTTAGCAGGGAAAGATGCAGAAGGAATATTTACTATAGGATCATTTTCTACTAACCTTGAAAATGAAAAAGTAA
AAGAGTTTGCAGAAAAATATAAAAAGAAATACAACCAAGATCCAAGTAGTTTTGCAGCTTATTCTTATGATGCAACTAAG
GTATTAATAGAAGGAATTAAAAAGGCTGGAAATGATAGAGAAAAAATCAATGAAGCTCTAAAAAATATGGGAACTTATAC
TGGAGTGACAGGAAATACTAAGTTTGAAAAAAGTGGAGATGCAGAAAAGAATCTAATAAGATTTATAGTTAAAGATGGAA
AATTTGTTGAATGGAAGAAATAG

Upstream 100 bases:

>100_bases
TTTATTTGAATGAAGTTTTTTTCCATGATATTCTGTTAAAGAATAATTAAAGAATAATTAAAAAATAGTTAAAAAACAAT
AAAAAGGGGAGAGAATTATA

Downstream 100 bases:

>100_bases
CATATGATTATAGAAGTATTAGTAAATGGAATTACCCTAGGCAGCATATATTGTTTGGCTGCCCTGGGTTTTAATATGAT
TTTTGGTGTATTAAAGATGT

Product: putative high-affinity branched-chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKFAKFKKKSLIALSSLLVLGSLVGCQSNPGEKEETIKVGLVAPLSGAMAQDGKSILNAAQLAVDEVNKEGGINKKKIEL
VYEDDKGEPKEAASIANKYSSDKDIMAVMGSFSAPCTLAGIPIYNKAKIPMMGPCGSAPALSGSSEYYRKVTPSDLTTGK
ELSKWMLKDMNYKKVAIIYVNNDYGKGVAESVDNFYKENGGQVVAKESYMPKTQDFGSIITKVKSKNPDIIIMGSFYGDA
GAFLKQAHNVGYNPKVAGPTPLLSKSLVDLAGKDAEGIFTIGSFSTNLENEKVKEFAEKYKKKYNQDPSSFAAYSYDATK
VLIEGIKKAGNDREKINEALKNMGTYTGVTGNTKFEKSGDAEKNLIRFIVKDGKFVEWKK

Sequences:

>Translated_380_residues
MKFAKFKKKSLIALSSLLVLGSLVGCQSNPGEKEETIKVGLVAPLSGAMAQDGKSILNAAQLAVDEVNKEGGINKKKIEL
VYEDDKGEPKEAASIANKYSSDKDIMAVMGSFSAPCTLAGIPIYNKAKIPMMGPCGSAPALSGSSEYYRKVTPSDLTTGK
ELSKWMLKDMNYKKVAIIYVNNDYGKGVAESVDNFYKENGGQVVAKESYMPKTQDFGSIITKVKSKNPDIIIMGSFYGDA
GAFLKQAHNVGYNPKVAGPTPLLSKSLVDLAGKDAEGIFTIGSFSTNLENEKVKEFAEKYKKKYNQDPSSFAAYSYDATK
VLIEGIKKAGNDREKINEALKNMGTYTGVTGNTKFEKSGDAEKNLIRFIVKDGKFVEWKK
>Mature_380_residues
MKFAKFKKKSLIALSSLLVLGSLVGCQSNPGEKEETIKVGLVAPLSGAMAQDGKSILNAAQLAVDEVNKEGGINKKKIEL
VYEDDKGEPKEAASIANKYSSDKDIMAVMGSFSAPCTLAGIPIYNKAKIPMMGPCGSAPALSGSSEYYRKVTPSDLTTGK
ELSKWMLKDMNYKKVAIIYVNNDYGKGVAESVDNFYKENGGQVVAKESYMPKTQDFGSIITKVKSKNPDIIIMGSFYGDA
GAFLKQAHNVGYNPKVAGPTPLLSKSLVDLAGKDAEGIFTIGSFSTNLENEKVKEFAEKYKKKYNQDPSSFAAYSYDATK
VLIEGIKKAGNDREKINEALKNMGTYTGVTGNTKFEKSGDAEKNLIRFIVKDGKFVEWKK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=330, Percent_Identity=29.6969696969697, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789867, Length=331, Percent_Identity=29.0030211480363, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41253; Mature: 41253

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAKFKKKSLIALSSLLVLGSLVGCQSNPGEKEETIKVGLVAPLSGAMAQDGKSILNAA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
QLAVDEVNKEGGINKKKIELVYEDDKGEPKEAASIANKYSSDKDIMAVMGSFSAPCTLAG
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEC
IPIYNKAKIPMMGPCGSAPALSGSSEYYRKVTPSDLTTGKELSKWMLKDMNYKKVAIIYV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
NNDYGKGVAESVDNFYKENGGQVVAKESYMPKTQDFGSIITKVKSKNPDIIIMGSFYGDA
ECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
GAFLKQAHNVGYNPKVAGPTPLLSKSLVDLAGKDAEGIFTIGSFSTNLENEKVKEFAEKY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
KKKYNQDPSSFAAYSYDATKVLIEGIKKAGNDREKINEALKNMGTYTGVTGNTKFEKSGD
HHHHCCCCCHHEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEKNLIRFIVKDGKFVEWKK
HHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MKFAKFKKKSLIALSSLLVLGSLVGCQSNPGEKEETIKVGLVAPLSGAMAQDGKSILNAA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
QLAVDEVNKEGGINKKKIELVYEDDKGEPKEAASIANKYSSDKDIMAVMGSFSAPCTLAG
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEC
IPIYNKAKIPMMGPCGSAPALSGSSEYYRKVTPSDLTTGKELSKWMLKDMNYKKVAIIYV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
NNDYGKGVAESVDNFYKENGGQVVAKESYMPKTQDFGSIITKVKSKNPDIIIMGSFYGDA
ECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCH
GAFLKQAHNVGYNPKVAGPTPLLSKSLVDLAGKDAEGIFTIGSFSTNLENEKVKEFAEKY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
KKKYNQDPSSFAAYSYDATKVLIEGIKKAGNDREKINEALKNMGTYTGVTGNTKFEKSGD
HHHHCCCCCHHEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AEKNLIRFIVKDGKFVEWKK
HHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]