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The map label for this gene is ddl

Identifier: 226947688

GI number: 226947688

Start: 581862

End: 582764

Strand: Direct

Name: ddl

Synonym: CLM_0535

Alternate gene names: 226947688

Gene position: 581862-582764 (Clockwise)

Preceding gene: 226947683

Following gene: 226947689

Centisome position: 14.0

GC content: 30.9

Gene sequence:

>903_bases
ATGAAAATTGGAGTTATAATGGGTGGAATATCTACAGAAAGAGAGGTTTCCTTGAATTCTGGTAGAGAAGTTATAAAATA
TTTAGAGCTTTTAGAGCATGAAATTATTCCTATTATTATAGATAAAAAAGAAGATGTTATGGAAAAAGCTAAGGGTATAG
ATTTTGCTTTCTTAGCATTGCATGGTAAATTTGGTGAAGATGGAACAGTTCAATCAGTTCTTCAAACTTTAGATATACCT
TATTCAGGATGTGGTCCTTTAACAAGCGCTATATGCATGGATAAGGATATGACTAAGAAAATTTTAAAATATGCTAATAT
AAATACAGCAGATTGGGTAAATGTATCTAGTGTAGAGAATATAGATTACGAAGCTATAGAAAAAATAGGGTACCCAGTGT
TCGTAAAACCTAATAGCGGTGGTTCTAGTGTAGCTACAAACTTAGTAAAAGATAAAGAGGGTATAAAAGAAGCAGTAGAA
CTTGCATTAAAATATGATAAAGAAGTTATGATAGAAAATTATACAAAAGGTGAAGAAATAACTTGCTGTATGCTAAATGG
CAAAATGTTACCAGTATTAGCTATAAGACCACACGCAGAATTCTTTGACTACACAGCAAAATATGCAGATGGTGGATCAG
ATGAAGTGGTTATAGAATTAGAAGAAAATCTTCATAAAAAGGTAGAAGAAATGGCCTTAGCTTGTTGGAAAGAATTGAAA
TGTGAAGTATATGTAAGGGTAGATATGATAGTTAAGGATGGAATTCCTTATGTATTAGAATTAAATACATTACCTGGTAT
GACAAAAAATAGCTTGTTCCCTAAGAGTGCTAATGCAGTAGGAATATCCTTTGCAGAATTATTAAATTCAATAGTTAAAT
ATTCATTAGAAGTAGAAAGATAA

Upstream 100 bases:

>100_bases
AGTAGGTTTAAATATAAATAGAAAGATGGTAAACATAAAGAACATTAAATCTAAAATTAATAGCAATGACATTAATAAAA
AGATATTGGAGGAGTAAATA

Downstream 100 bases:

>100_bases
TAAAATAAATTAGAATAAAAAATACTTATAGATTGTAAGTGATCTATAAGTATTTTTTATTTGAATGAAGTTTTTTTCCA
TGATATTCTGTTAAAGAATA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSVENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER

Sequences:

>Translated_300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSVENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
>Mature_300_residues
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLALHGKFGEDGTVQSVLQTLDIP
YSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSVENIDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVE
LALKYDKEVMIENYTKGEEITCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=307, Percent_Identity=38.7622149837134, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=28.9398280802292, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CLOB1 (A7FPH8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382844.1
- ProteinModelPortal:   A7FPH8
- SMR:   A7FPH8
- STRING:   A7FPH8
- GeneID:   5397009
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0493
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   GNEMIAH
- ProtClustDB:   PRK01372
- BioCyc:   CBOT441770:CLB_0493-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33349; Mature: 33349

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLAL
CEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLQTLDIPYSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSVEN
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCEECCCCCC
IDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVELALKYDKEVMIENYTKGEEI
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCE
TCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
EEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
EEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKIGVIMGGISTEREVSLNSGREVIKYLELLEHEIIPIIIDKKEDVMEKAKGIDFAFLAL
CEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEEE
HGKFGEDGTVQSVLQTLDIPYSGCGPLTSAICMDKDMTKKILKYANINTADWVNVSSVEN
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCEECCCCCC
IDYEAIEKIGYPVFVKPNSGGSSVATNLVKDKEGIKEAVELALKYDKEVMIENYTKGEEI
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCE
TCCMLNGKMLPVLAIRPHAEFFDYTAKYADGGSDEVVIELEENLHKKVEEMALACWKELK
EEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
CEVYVRVDMIVKDGIPYVLELNTLPGMTKNSLFPKSANAVGISFAELLNSIVKYSLEVER
EEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA