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The map label for this gene is rpiR [C]

Identifier: 226947494

GI number: 226947494

Start: 313638

End: 314405

Strand: Reverse

Name: rpiR [C]

Synonym: CLM_0328

Alternate gene names: 226947494

Gene position: 314405-313638 (Counterclockwise)

Preceding gene: 226947495

Following gene: 226947491

Centisome position: 7.57

GC content: 28.12

Gene sequence:

>768_bases
ATGATAGATGACTTAGATGAAAAAATTTTAAAAAAACTAACTCCAAATGAAAAGGATTTATTAAATTATATTAATTCTAA
TAAATTTGAGATTTATAAAATGAGTATACATGAATTTGCACAAAGCACCTATGTATCTACTGCAACCGTTTTACGATTGT
GTAAAAAATTAGGTTTTAATGGCTTTAATGAACTAAAATTTAATCTAAAAAAATCTCTAACTTTAAAAAATAAGGGCTAT
GATTCTTCTATAGGACTTAATGGACTACTTTCTAGACGTCTTTTGGAATTAAAAGAAACTATGCTATCTTTGAATCTAGA
ACAACTAGATAGCATAGTAGACTATCTTTGCAGCGATAAGAATATTCATATATTTGGTAGGGGCCTTACTCAAATGTCTT
TAGAATATCTTTATAATATATTAATATCCTTAAACAGACAATGTATGTTTTACCTTGATCCCCCACTTGTTTACCAAACT
GCTTCTCAAATGGATGAGAATGATGTATTTATAATTGGAAGCTTTGGAGGATCCACAGACCCTATTGTTAAAGCAGTAGA
AATTAGTAAGAACAATACTGGCACTGTTATAGCTATTACTTCTAATCCTAATAGTGAGTTAGCTAAAAAAGCTGATATCG
TTTTAATTGGGAAAACCCAAAATAGATTTTTTTCTGGCATTGATATTAACTCCAGATTATCTATACAATTCATTGTAGAA
ATAATTATAGAATTATATATGTCACGTATGAATATTGTTTCGCATTAG

Upstream 100 bases:

>100_bases
AACCTGTATTTTCTATATTGTAGATATGATAATAGAATGTTATATTAAATATTTAGATAAATCAAAATTTAATAATTTAT
ATTAAAAGGAGAACCACGTC

Downstream 100 bases:

>100_bases
ATTTATAAAAATAGCATCATTTTAAGTTGCTATTTTTATATTTATATAATTTAATATTTCATAGCTTCTTTTACTTGCTT
TGCTACTTTTTCAACTTTAG

Product: RpiR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MIDDLDEKILKKLTPNEKDLLNYINSNKFEIYKMSIHEFAQSTYVSTATVLRLCKKLGFNGFNELKFNLKKSLTLKNKGY
DSSIGLNGLLSRRLLELKETMLSLNLEQLDSIVDYLCSDKNIHIFGRGLTQMSLEYLYNILISLNRQCMFYLDPPLVYQT
ASQMDENDVFIIGSFGGSTDPIVKAVEISKNNTGTVIAITSNPNSELAKKADIVLIGKTQNRFFSGIDINSRLSIQFIVE
IIIELYMSRMNIVSH

Sequences:

>Translated_255_residues
MIDDLDEKILKKLTPNEKDLLNYINSNKFEIYKMSIHEFAQSTYVSTATVLRLCKKLGFNGFNELKFNLKKSLTLKNKGY
DSSIGLNGLLSRRLLELKETMLSLNLEQLDSIVDYLCSDKNIHIFGRGLTQMSLEYLYNILISLNRQCMFYLDPPLVYQT
ASQMDENDVFIIGSFGGSTDPIVKAVEISKNNTGTVIAITSNPNSELAKKADIVLIGKTQNRFFSGIDINSRLSIQFIVE
IIIELYMSRMNIVSH
>Mature_255_residues
MIDDLDEKILKKLTPNEKDLLNYINSNKFEIYKMSIHEFAQSTYVSTATVLRLCKKLGFNGFNELKFNLKKSLTLKNKGY
DSSIGLNGLLSRRLLELKETMLSLNLEQLDSIVDYLCSDKNIHIFGRGLTQMSLEYLYNILISLNRQCMFYLDPPLVYQT
ASQMDENDVFIIGSFGGSTDPIVKAVEISKNNTGTVIAITSNPNSELAKKADIVLIGKTQNRFFSGIDINSRLSIQFIVE
IIIELYMSRMNIVSH

Specific function: Regulatory Protein Involved In Rpib Gene Repression. Also Involved In Als Operon Repression. [C]

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI87082369, Length=229, Percent_Identity=20.9606986899563, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788767, Length=229, Percent_Identity=24.4541484716157, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01418 HTH_6; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 28962; Mature: 28962

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDLDEKILKKLTPNEKDLLNYINSNKFEIYKMSIHEFAQSTYVSTATVLRLCKKLGFN
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
GFNELKFNLKKSLTLKNKGYDSSIGLNGLLSRRLLELKETMLSLNLEQLDSIVDYLCSDK
CCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
NIHIFGRGLTQMSLEYLYNILISLNRQCMFYLDPPLVYQTASQMDENDVFIIGSFGGSTD
CEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCC
PIVKAVEISKNNTGTVIAITSNPNSELAKKADIVLIGKTQNRFFSGIDINSRLSIQFIVE
CCEEEEEECCCCCCEEEEEECCCCHHHHHHCCEEEEECCCCCEECCCCCCCCHHHHHHHH
IIIELYMSRMNIVSH
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDDLDEKILKKLTPNEKDLLNYINSNKFEIYKMSIHEFAQSTYVSTATVLRLCKKLGFN
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
GFNELKFNLKKSLTLKNKGYDSSIGLNGLLSRRLLELKETMLSLNLEQLDSIVDYLCSDK
CCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
NIHIFGRGLTQMSLEYLYNILISLNRQCMFYLDPPLVYQTASQMDENDVFIIGSFGGSTD
CEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCC
PIVKAVEISKNNTGTVIAITSNPNSELAKKADIVLIGKTQNRFFSGIDINSRLSIQFIVE
CCEEEEEECCCCCCEEEEEECCCCHHHHHHCCEEEEECCCCCEECCCCCCCCHHHHHHHH
IIIELYMSRMNIVSH
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]