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The map label for this gene is eno [H]

Identifier: 226947451

GI number: 226947451

Start: 267160

End: 268455

Strand: Direct

Name: eno [H]

Synonym: CLM_0280

Alternate gene names: 226947451

Gene position: 267160-268455 (Clockwise)

Preceding gene: 226947450

Following gene: 226947452

Centisome position: 6.43

GC content: 33.64

Gene sequence:

>1296_bases
ATGAAAAATTATATTGAAATAGTAGATGTATATGCAAGACAAATCTTAGATTCAAGATGCAACCCTACAGTAGAAGTAGA
AGTAGAATTAGAAGACGGAACAGTAGGAGTAGCAGCAGTTCCATCAGGAGCTTCTACAGGAGCTTTTGAAGCAGTTGAAT
TAAGAGATGGAGATAAATCTCAATACTTAGGTAAAGGGGTTTTAAAAGCTGTAGATAATGTAAACACTACAATAGCAGAT
GAACTTGTTGGAATGAATGTTTTAGATCAAGTAGCTATTGATAAAACTATGATAGAATTAGATGGAACAGACAATAAAGC
AAAATTAGGTGCAAATGCAATGTTAGGAGTATCTTTAGCTTGTGCAAAAGCTGCAGCTAACTCTTTAGGAATGAGTTTAT
ACCAATACATAGGAGGAGTAAACGGAAAAGTTTTACCAGTACCTATGATGAACATAATAAATGGTGGAAAACATGCGGAC
AACAATGTTGACCTTCAAGAATTCATGATAATGCCAGCAGGAGCTCCTTCTTTCAGCGAAGCTTTAAGAATGTGTTCAGA
AGTATATCATGCATTAAAATCAACATTAAAAGCACAAGGATATGATACAGGGGTAGGCGACGAAGGTGGATTTGCTCCAA
ACTTAAAATCAAATGAAGAAGCTATAGTAGTTATAATAGAAGCTATAAAAAAAGCTGGATATACTCCAGGAAAAGATATA
TTCATAGCTTTAGATCCAGCTTCATCAGAAATATTTGAAGATGGAAAATACAACCTAGCAGGAGAAGGAAGAGTATTAAC
TCCAGAAGAAATGGCAAACTACTATGTAGAATTAGCAGAAAAATACCCAATAATCTCTATAGAAGACGGTATGGCAGAAG
AAGATTGGGATGGTTGGAAAATCCTTACTGAAAAAATAGGAAACAAAGTTCAATTAGTAGGAGACGATTTATTCGTTACA
AATACTGAAAGATTATCAAAAGGAATAAAATTAGGAGTTGCAAACTCAATTCTTATAAAACTTAACCAAATAGGAACATT
AACAGAAACATTAAATGCCATAGAAATGGCAGAAAGAGCTGGATATACAGCAGTAGTATCTCATAGATCAGGAGAAACTG
AAGATACAACAATAGCTGACTTAGTTGTTGCAGTAAATGCAGGACAAATAAAAACAGGTGCTCCAGCAAGATCAGAAAGA
GTTGCTAAATACAATCAATTATTAAGAATAGAAGAAGAACTTAATGATATGGGAGAATACAGAGGATTAAAAGCATTCTA
CAATATCAATAAATAA

Upstream 100 bases:

>100_bases
CTTCCTAAACCTATTGACATGACAGGGAATAGTCTTATTAAATAATAGAGAAACTATATTTAAGATAAAATAATTTTTTT
AAAAATAAGGAGGACCCAAC

Downstream 100 bases:

>100_bases
TAATTTTTAAAACAAGGGGCTGCTCATAGTATGGGTAGTCCTTTAATCTATAAAAATGTAAATATAAAAGTATATATGTA
ATTAAAATATAAATTAATCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSQYLGKGVLKAVDNVNTTIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGKDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Sequences:

>Translated_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSQYLGKGVLKAVDNVNTTIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGKDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK
>Mature_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSQYLGKGVLKAVDNVNTTIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGKDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.8348623853211, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=52.4361948955916, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=52.4361948955916, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=47.2941176470588, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=25.7062146892655, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=25.7062146892655, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=25.7062146892655, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=63.6792452830189, Blast_Score=524, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.0603248259861, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.0603248259861, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=48.6772486772487, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.810623556582, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=50.9049773755656, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=50.9049773755656, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=50.6787330316742, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=53.1177829099307, Blast_Score=388, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580918, Length=425, Percent_Identity=53.8823529411765, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580916, Length=425, Percent_Identity=53.8823529411765, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580920, Length=425, Percent_Identity=53.8823529411765, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580914, Length=425, Percent_Identity=53.8823529411765, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281360527, Length=418, Percent_Identity=54.3062200956938, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=418, Percent_Identity=54.3062200956938, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46393; Mature: 46393

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCHH
QYLGKGVLKAVDNVNTTIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGKDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCEE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC
>Mature Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCHH
QYLGKGVLKAVDNVNTTIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGKDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCEE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA