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The map label for this gene is maeB [C]

Identifier: 226947431

GI number: 226947431

Start: 243075

End: 244247

Strand: Direct

Name: maeB [C]

Synonym: CLM_0260

Alternate gene names: 226947431

Gene position: 243075-244247 (Clockwise)

Preceding gene: 226947430

Following gene: 226947435

Centisome position: 5.85

GC content: 31.2

Gene sequence:

>1173_bases
ATGGATATCAAAGAAAAATCACTAATGGTACATAAAAAATTTAAAGGCAAATTATCTATAGAAGGAAAAATACAAGTAAA
AAATAAAGAAGATTTATCTATAGCATATACTCCAGGAGTTGCAGAACCCTGCGTAAAAATAAATGAAGATAAATCTTTAG
TTTATGAATATACAATGAAGGGAAATACAGTAGCTGTAGTAACTAATGGTACAGCAGTTTTAGGCTTAGGGGATATAGGG
CCTTATGCAGGATTGCCTGTTATGGAAGGAAAAGCTCTTTTATTTAAGGAATTTGCAGATATAGATTCATTTCCAATTTG
TATAGATAGTAAAGACCCTGAAGAAATTATAAAAACAGTTAAACTTATAGCTCCAGGATTTGGAGGAATAAATTTAGAGG
ACATAAAAGCACCAGAATGTTTTTATATAGAAAAAAAATTAAAAGAGGAATTAGATATTCCAGTGTTTCATGATGATCAG
CATGGAACAGCCATAGTTGTATTAGCAGGAATATATAATGCTTTAAGACTTGTAGGGAAAAAATTAGAAGAAGCAAGAAT
AGTTATAAATGGTGCAGGCTCTGCAGGAATATCCATATGCAAATTGTTATTACAAGCAGGTGCTAAAAACATAATAATGT
GTGATAAAGAAGGATCTTTAGTAAAAGGTAATAGTAATTTAAATGAAGCACAAAAATTAATAGTCGAAGTAACAAATAAG
GAAAATGAAAAAGGAACATTAAAAGACGTAATAAAAGGAAAAGATGTATTTATAGGTGTATCAGCCCCAAATATACTTAC
AGAAGAAATGATAACCTCTATGAACAAAGATTCCATAGTATTTGCTATGGCAAATCCAACTCCAGAAATAATGCCGGATA
ATGCAAAAAAGGCAGGAGCTAGAGTAGTAGCTACAGGAAGATCTGATTTCCCAAATCAAATAAATAATGTGTTGGTATTT
CCAGGAGTTTTTAGAGGAGCATTAGATGTAAGATCTAGAGTTATAAATGAGGAAATGAAACTAGCAGCATCTAAAGCTAT
AGCTTCTTTGGTACAGGATAATGAATTAAATGAAGAGTACATAATACCAGGAGCCTTTGATAAAAGAGTAGCAGAAGTTG
TGGCAGAAGAAGTAAAAAAAGTAGCCATAAAAATGGGACTATCAAAATTATAA

Upstream 100 bases:

>100_bases
CGCTCTTTACTCCCATTTTGAAGAAGATGGAAGTATTAGAGCAGGTAGTCATCGGATAAATTAAATAAATCCTATATACA
ATGAAAGAGGGGATAATAAT

Downstream 100 bases:

>100_bases
AATAAGGAAGTTGCGTAGCAACTTCCTTATTTTAAGTTTCTTCTTAAAAAGTCTAGAGCTCTAGTTACAGTTATATTTCT
TATAAACTGTCTGTTACCAG

Product: malate dehydrogenase (oxaloacetate-decarboxylating)

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGAKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMITSMNKDSIVFAMANPTPEIMPDNAKKAGARVVATGRSDFPNQINNVLVF
PGVFRGALDVRSRVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVAIKMGLSKL

Sequences:

>Translated_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGAKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMITSMNKDSIVFAMANPTPEIMPDNAKKAGARVVATGRSDFPNQINNVLVF
PGVFRGALDVRSRVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVAIKMGLSKL
>Mature_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGAKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMITSMNKDSIVFAMANPTPEIMPDNAKKAGARVVATGRSDFPNQINNVLVF
PGVFRGALDVRSRVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVAIKMGLSKL

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=411, Percent_Identity=26.7639902676399, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI62420880, Length=411, Percent_Identity=26.7639902676399, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI239049447, Length=411, Percent_Identity=26.7639902676399, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4505145, Length=411, Percent_Identity=27.9805352798054, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI4505143, Length=359, Percent_Identity=27.5766016713092, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI270265879, Length=370, Percent_Identity=27.8378378378378, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788806, Length=410, Percent_Identity=48.2926829268293, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI87081919, Length=487, Percent_Identity=25.0513347022587, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17537199, Length=450, Percent_Identity=26.8888888888889, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=345, Percent_Identity=26.3768115942029, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281362672, Length=442, Percent_Identity=28.0542986425339, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356279, Length=442, Percent_Identity=28.0542986425339, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362674, Length=442, Percent_Identity=28.0542986425339, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78707242, Length=431, Percent_Identity=25.754060324826, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78707238, Length=431, Percent_Identity=25.754060324826, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78707240, Length=431, Percent_Identity=25.754060324826, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78707232, Length=373, Percent_Identity=26.2734584450402, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363503, Length=373, Percent_Identity=26.2734584450402, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281363505, Length=373, Percent_Identity=26.2734584450402, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78707236, Length=373, Percent_Identity=26.2734584450402, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42274; Mature: 42274

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00331 MALIC_ENZYMES ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHEEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEARIVINGAGSAGISICKLLLQAGAKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMITSMNKDSIVFAMANPTPEIMPDNAKKAGA
CCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
RVVATGRSDFPNQINNVLVFPGVFRGALDVRSRVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVAIKMGLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHEEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEARIVINGAGSAGISICKLLLQAGAKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMITSMNKDSIVFAMANPTPEIMPDNAKKAGA
CCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
RVVATGRSDFPNQINNVLVFPGVFRGALDVRSRVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVAIKMGLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]