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The map label for this gene is pyrG [H]

Identifier: 226947355

GI number: 226947355

Start: 163374

End: 164975

Strand: Direct

Name: pyrG [H]

Synonym: CLM_0175

Alternate gene names: 226947355

Gene position: 163374-164975 (Clockwise)

Preceding gene: 226947354

Following gene: 226947356

Centisome position: 3.93

GC content: 31.34

Gene sequence:

>1602_bases
ATGAGCACTAAATATATATTTGTAACAGGTGGTGTTGTTTCTTCATTAGGGAAAGGAATAACAGCAGCTTCTTTAGGAAG
ACTTTTAAAAAATAGAGGACTTAAAGTTTCAATACAAAAATTTGATCCATATATAAATATAGATCCAGGAACAATGAGTC
CTTATCAACATGGTGAAGTATTTGTAACAGAGGATGGTGCAGAAACAGATTTAGATTTAGGCCATTATGAAAGGTTTATA
GATGAAAATTTAAGTCAAAATAGTAATGTAACTACAGGAAAGATTTACTGGTCTGTTATATCTAAAGAAAGAAAAGGTGA
TTATTTAGGTGCTACAGTGCAGGTAATACCGCATATAACTACAGAAATAAAATCTAGAGTATATAGAGTCGCTAAAGAAA
AAGAAGTAGATGTAGTTATAACTGAAATAGGTGGAACTATAGGAGATATAGAATCTCTACCTTTTTTAGAATCAATAAGG
CAAATCAAATATGAGGTTGGAAGAGAAAATGTATGCTTTATTCATGTAACCTTACTACCCTTTCTAGGAAAAGCAGGTGA
GTTAAAGACAAAGCCTACACAACACTCAGTTAAAGAACTTAGAGGAATAGGGATACAGCCAGATATCATAGTTTGTCGAT
CAGAAAAACAGATACCAGAGGAACTAAAGGGGAAAATAGGATTATTTTGTAATGTAGATAAAGAAGATGTCATACAAAAT
TTAGACGCAGAAAATCTTTATGAAGTACCATTAATGCTACATAAGGAAAATTTAGATACTTTAGTATGTAAGAAATTAGG
CCTAAATTATAGTAAAATAGATAACACGGAATGGATATCAATGGTAAATAATGCAAAAAACCTTTCTAAGAATACTACCA
TAGCTTTGGTAGGGAAGTATGTAGAATTACATGATGCTTATATATCAATAGTGGAAGCTTTAAACCATGGTGGTTATGCT
AATGATACTAAGGTAAATATAAAATGGATAAATTCTGAAGAGGTAAATGAAAATAACTTTGAACAATGTTTAAAAGATGT
GGATGGTATATTGGTACCAGGTGGATTTGGTGATAGAGGAATAGAAGGTAAAATATGGGCTGCTAAATGGGCAAGAGAAA
ATAAAATACCTTTTTTAGGAATATGTCTTGGAATGCAATGTGCAGTAATAGAGTTTGCCAGAAATGTATTAAATTATAAA
GGAGCTCATAGTGCAGAGATAGATCCAAATACAAAATATCCAGTAATAGATTTAATGCCAGAACAGAAAGATATAGATAA
TATGGGTGGAACTATGAGATTGGGAGCTTATCCTTGTAAATTACTTAAGGGTACAAATTCAGAAAAAGCTTATGGTCAAG
AGGTTGTATACGAAAGACATAGACATAGATATGAATTTAATAATGAATATAGGAGTAAATTAACAGAAGCAGGTTTAATA
TTGTCAGGAACAAGTCCAGACGATAGATTAGTAGAAATAGTAGAGATAAATAATCATCCTTGGTATGTGGCTGTTCAATT
TCACCCAGAATTAAAATCAAGACCCAATAAGCCACATCCATTATTTAAAAACTTTGTAAAGGCCACTTTGGATAAAAAAT
AA

Upstream 100 bases:

>100_bases
TAATGATGTCAATAGATTTTATGTAAATTTAGTGCACAATAATACAAACTTAAACTTTGGAGGTGAAAACTGCTTTATTA
TATATTAAAGCAGGGGAGAT

Downstream 100 bases:

>100_bases
ATTTTGTATACTAGTTAAAATTTAATATATGTTAATAAAGAACACCATCAGAATTTATAAAAATAAATAATTTTGGTGTT
GTTTTTTTATTTATCCGATG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFI
DENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEVNENNFEQCLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEVVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKNFVKATLDKK

Sequences:

>Translated_533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFI
DENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEVNENNFEQCLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEVVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKNFVKATLDKK
>Mature_532_residues
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
ENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQ
IKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQNL
DAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYAN
DTKVNIKWINSEEVNENNFEQCLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKG
AHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEVVYERHRHRYEFNNEYRSKLTEAGLIL
SGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKNFVKATLDKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=48.2758620689655, Blast_Score=504, Evalue=1e-143,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=48.9051094890511, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=48.9051094890511, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=48.9051094890511, Blast_Score=501, Evalue=1e-142,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=56.0296846011132, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI25148299, Length=600, Percent_Identity=38.3333333333333, Blast_Score=413, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=45.3405017921147, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=45.5357142857143, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24664469, Length=550, Percent_Identity=48.9090909090909, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI21357815, Length=495, Percent_Identity=47.0707070707071, Blast_Score=434, Evalue=1e-122,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59945; Mature: 59814

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIDENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCHHHCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEVNENNFEQCLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEIDPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEVVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKNFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIDENLSQNSNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCHHHCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEVNENNFEQCLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEIDPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEVVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKNFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA