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The map label for this gene is regA [H]

Identifier: 226947323

GI number: 226947323

Start: 125597

End: 126595

Strand: Direct

Name: regA [H]

Synonym: CLM_0143

Alternate gene names: 226947323

Gene position: 125597-126595 (Clockwise)

Preceding gene: 226947322

Following gene: 226947324

Centisome position: 3.02

GC content: 27.23

Gene sequence:

>999_bases
ATGGCAGTTTCCATTAAAGATGTAGCTAAAGAGGCACAGGTTTCCATAGCTACTGTTTCAAGAGTATTAAATAATATAGA
TGTTGTAAATGAAGATACTAAAAAGAAAGTATTAGATGCAATAGACAAATTAGGATATAGACCTAATATAGTAGCTAGAA
GTCTAAAAACACAAAAATCTAAAACTATAGGCATAATAATACCAGATATATCAAATCAATTTTATCCTGAAATAGTAAGG
GGAGCTGAAGATGTAGCTAACATATATGATTATAATATAATGTTATGTAATACAGATTTAGATCCAGAAAAAGAAATGGA
ATACCTAAGAGTTTTAAGGGAAAAAATGGCAGATGGTGTTTTATATATGAGTAATTCTTTAGAAAAAAATATATTAGAAC
TAATTAAAAGTTTGCAATTACCAGTAGTTTTAGTAGAAACAAAAGATAGTGAAGAAAAGCTACCAAGTGTAACTATAGAT
AATTTAAAAGCATCTTTAGATGCTACAGAATACTTAATTAATAAAGGTAATAAGAATATAGCTTACATAGGATTACATGA
GGATATGGCAAATGCAGCAGTTTTAAGATATGAAGGATATAAATTAGCATTAAGGAAAAATAATATTAAAGAAAATAAAG
ACCTAGTTTATTTAGGTGGCATGAAAGTTAGAGATGGTTACGAAGGTATAAATAAAATATTAGAAAAGGAAAAAATAGAT
GCTATTTTTTGTGCTGATGATGAAATAGCTATGGGAGCAATAAATGCATTAAGGGATAAAAATATAAAAGTTCCTGATGA
GATAGATGTTATGGGATTTAACAATATATATTCCTCTAATGTTTTTTACCCTAAATTAACAACTATAGGGCAACCAATGT
ATGATATGGGTTCTGTAGGAATGAGAATGCTTATAAAAATAATAAATAAAGAAGAATTAGAGAATGATAATTATGTATTG
CAGCACAAAATAGTAGAAAGAGATTCCTGTAAAAAATAG

Upstream 100 bases:

>100_bases
TTTATTATAATAAGTATAATAAGGGAAAACTACTAAACAAGAGAAAATTTCATATAGATAAATATGTTAGATTTCAATAA
ATAATAGGGAGTGATTTTAA

Downstream 100 bases:

>100_bases
ATAGTTATTAATATATTTTATAAAAAAGATGTGTAGATAAATAGCACATCTTTTTTTATTTTATCAAAATTGTTAAAAGC
TTAAAGGAAACAATAAAACC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVR
GAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTID
NLKASLDATEYLINKGNKNIAYIGLHEDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
AIFCADDEIAMGAINALRDKNIKVPDEIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVL
QHKIVERDSCKK

Sequences:

>Translated_332_residues
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVR
GAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTID
NLKASLDATEYLINKGNKNIAYIGLHEDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
AIFCADDEIAMGAINALRDKNIKVPDEIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVL
QHKIVERDSCKK
>Mature_331_residues
AVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKSKTIGIIIPDISNQFYPEIVRG
AEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGVLYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDN
LKASLDATEYLINKGNKNIAYIGLHEDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKIDA
IFCADDEIAMGAINALRDKNIKVPDEIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVGMRMLIKIINKEELENDNYVLQ
HKIVERDSCKK

Specific function: Involved in the regulation of amylase production [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=31.9277108433735, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=31.6265060240964, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.5151515151515, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=30.2931596091205, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=30.2547770700637, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=29.0909090909091, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=27.6276276276276, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790715, Length=329, Percent_Identity=26.1398176291793, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787580, Length=331, Percent_Identity=26.5861027190332, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=25.6716417910448, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790689, Length=288, Percent_Identity=26.3888888888889, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789456, Length=312, Percent_Identity=25.9615384615385, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=23.2628398791541, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1786268, Length=281, Percent_Identity=23.4875444839858, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37555; Mature: 37424

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KTIGIIIPDISNQFYPEIVRGAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGV
CEEEEEECCCCCCHHHHHHHCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCE
LYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDNLKASLDATEYLINKGNKNI
EEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
AYIGLHEDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
EEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCC
AIFCADDEIAMGAINALRDKNIKVPDEIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVG
EEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHH
MRMLIKIINKEELENDNYVLQHKIVERDSCKK
HHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC
>Mature Secondary Structure 
AVSIKDVAKEAQVSIATVSRVLNNIDVVNEDTKKKVLDAIDKLGYRPNIVARSLKTQKS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KTIGIIIPDISNQFYPEIVRGAEDVANIYDYNIMLCNTDLDPEKEMEYLRVLREKMADGV
CEEEEEECCCCCCHHHHHHHCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCE
LYMSNSLEKNILELIKSLQLPVVLVETKDSEEKLPSVTIDNLKASLDATEYLINKGNKNI
EEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCEEHHHHHHHHHHHHHHHHCCCCEE
AYIGLHEDMANAAVLRYEGYKLALRKNNIKENKDLVYLGGMKVRDGYEGINKILEKEKID
EEEEECCCCCCEEEEEECCEEEEEECCCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCC
AIFCADDEIAMGAINALRDKNIKVPDEIDVMGFNNIYSSNVFYPKLTTIGQPMYDMGSVG
EEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHCCCCCCCCHHHCCCCHHHHHHHH
MRMLIKIINKEELENDNYVLQHKIVERDSCKK
HHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]