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The map label for this gene is dacA [H]

Identifier: 226225362

GI number: 226225362

Start: 2864422

End: 2865240

Strand: Direct

Name: dacA [H]

Synonym: Lm4b_02785

Alternate gene names: 226225362

Gene position: 2864422-2865240 (Clockwise)

Preceding gene: 226225329

Following gene: 226225365

Centisome position: 98.34

GC content: 40.78

Gene sequence:

>819_bases
ATGAAAATACATAAACTAACTTGGGTGCTATTAATGGGGCTACTACTGCTCTCATCCTGTTCCACGAAGCAACCAAACCT
ATATTTGTCAGCAAATGCCGCCGCGGTTTATTCAGTCGAAAATGGTGAACCGTTATATGAAGAAAACGCTGATAAAGTAA
TGCCGATTGCCAGTTTGACCAAATTAATGACAGCTTTTTTAGTTTTAGAGGCTGTGGATAACAATGAATTATCGTGGGAT
GAAAAGCTTGATCTTGTTCGATTGGATGATCCTTCCGCGGTTTCCCTTTACGCGATTACACAAAAGAGAACGTGGTCCGT
CAGAGATTTATACAGCGCGATGCTCACTATGTCTGCAAATGATGCCGCGGAAACACTGGGAGATCGTTTAGACGGAGCTA
ATTTTCCCAAAGAAATGAATAACCAGGCTAAAAAATTAGGTATGTCGAGTAAGACTACATTTGTCAGTGCTAGCGGACTT
GATGTTGACGGAAAATCCGCTGTTTCTACCACAAAGGATTTATTTTTGCTATCATCTAAATTAATTTCCACTCATCCTGA
AGTGTTAGAGACAACATCCAAATCTAGTGTCACCACTGACAAAGGCGCCAAACTTGAATCCACCAATGACTCACTCGGTT
CGATTCAAGGCTTAGATGGACTAAAAACTGGATTTACAGATGAAGCAGGCTATTGCTTTATTGGAACAGCTGAACGTGGA
GGAAAACGTGTGATTTCGATTGTCCTAGATGCGGGAACTGCGGAAAAACGATTTAAAGATACAGAGAAGCTGATGGAGGT
TGGATTTAAAGAAAATTAA

Upstream 100 bases:

>100_bases
AAACTTAAATTTTCTCGTTAAAGCTTTAACTTATCCTTCCACTTCCTTGGCTTTAACTCAATTCTTTTTATCTATACTAA
AGTTAAAAGGAGGAGAAAAA

Downstream 100 bases:

>100_bases
CGAATGAAAAGAACCTCTCTTTCGCTGAGAAGTTCTTTTTTAAATAGTTCAGCATCAACTCTTTAAAGCGCATAGATACT
TTTCCATGTATACATCTTTA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKIHKLTWVLLMGLLLLSSCSTKQPNLYLSANAAAVYSVENGEPLYEENADKVMPIASLTKLMTAFLVLEAVDNNELSWD
EKLDLVRLDDPSAVSLYAITQKRTWSVRDLYSAMLTMSANDAAETLGDRLDGANFPKEMNNQAKKLGMSSKTTFVSASGL
DVDGKSAVSTTKDLFLLSSKLISTHPEVLETTSKSSVTTDKGAKLESTNDSLGSIQGLDGLKTGFTDEAGYCFIGTAERG
GKRVISIVLDAGTAEKRFKDTEKLMEVGFKEN

Sequences:

>Translated_272_residues
MKIHKLTWVLLMGLLLLSSCSTKQPNLYLSANAAAVYSVENGEPLYEENADKVMPIASLTKLMTAFLVLEAVDNNELSWD
EKLDLVRLDDPSAVSLYAITQKRTWSVRDLYSAMLTMSANDAAETLGDRLDGANFPKEMNNQAKKLGMSSKTTFVSASGL
DVDGKSAVSTTKDLFLLSSKLISTHPEVLETTSKSSVTTDKGAKLESTNDSLGSIQGLDGLKTGFTDEAGYCFIGTAERG
GKRVISIVLDAGTAEKRFKDTEKLMEVGFKEN
>Mature_272_residues
MKIHKLTWVLLMGLLLLSSCSTKQPNLYLSANAAAVYSVENGEPLYEENADKVMPIASLTKLMTAFLVLEAVDNNELSWD
EKLDLVRLDDPSAVSLYAITQKRTWSVRDLYSAMLTMSANDAAETLGDRLDGANFPKEMNNQAKKLGMSSKTTFVSASGL
DVDGKSAVSTTKDLFLLSSKLISTHPEVLETTSKSSVTTDKGAKLESTNDSLGSIQGLDGLKTGFTDEAGYCFIGTAERG
GKRVISIVLDAGTAEKRFKDTEKLMEVGFKEN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=250, Percent_Identity=33.2, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082022, Length=257, Percent_Identity=32.6848249027237, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082054, Length=253, Percent_Identity=32.0158102766798, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786851, Length=253, Percent_Identity=27.6679841897233, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 29501; Mature: 29501

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHKLTWVLLMGLLLLSSCSTKQPNLYLSANAAAVYSVENGEPLYEENADKVMPIASLT
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHH
KLMTAFLVLEAVDNNELSWDEKLDLVRLDDPSAVSLYAITQKRTWSVRDLYSAMLTMSAN
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHCCCC
DAAETLGDRLDGANFPKEMNNQAKKLGMSSKTTFVSASGLDVDGKSAVSTTKDLFLLSSK
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LISTHPEVLETTSKSSVTTDKGAKLESTNDSLGSIQGLDGLKTGFTDEAGYCFIGTAERG
HHHCCHHHHHCCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GKRVISIVLDAGTAEKRFKDTEKLMEVGFKEN
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIHKLTWVLLMGLLLLSSCSTKQPNLYLSANAAAVYSVENGEPLYEENADKVMPIASLT
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHH
KLMTAFLVLEAVDNNELSWDEKLDLVRLDDPSAVSLYAITQKRTWSVRDLYSAMLTMSAN
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHCCCC
DAAETLGDRLDGANFPKEMNNQAKKLGMSSKTTFVSASGLDVDGKSAVSTTKDLFLLSSK
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LISTHPEVLETTSKSSVTTDKGAKLESTNDSLGSIQGLDGLKTGFTDEAGYCFIGTAERG
HHHCCHHHHHCCCCCCCCCCCCCEECCCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GKRVISIVLDAGTAEKRFKDTEKLMEVGFKEN
CCEEEEEEEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]