Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 226225352

GI number: 226225352

Start: 2856027

End: 2856743

Strand: Reverse

Name: gidB

Synonym: Lm4b_02775

Alternate gene names: 226225352

Gene position: 2856743-2856027 (Counterclockwise)

Preceding gene: 226225353

Following gene: 226225351

Centisome position: 98.08

GC content: 41.42

Gene sequence:

>717_bases
ATGAATCCAGAGCAATTCCAAACAGCACTTGCTGAAAAAGGAATAGAATTAAGCGACACGCAACTAAAACAATTCCACGA
TTACTTCGAAATGTTAGTAGAATGGAATGAAAAAATGAATTTAACAGCTATTACTGATGAAAAAGAAGTGTATTTAAAGC
ACTTTTACGATTCTATCTCTGCCGCGTTTTATGTAGACTTTACTAAGTTCGATACGATTTGTGATGTCGGGGCTGGCGCT
GGTTTTCCAAGTCTCCCAATTAAAATCTGCTTCCCGCATCTAAAAGTAAGCATCGTAGACTCCTTGAAAAAACGTATGAC
TTTCCTTGATGCACTAGCAGAAAAATTAGGCCTAACGGATGTGCATTTTTATCATGATCGCGCTGAAACATTTGGTCAAA
ACAAAGCGCACCGCGAAAAATACGACCTTGTAACCGCACGTGCCGTAGCAAGAATGAGCGTATTATCCGAACTGTGCATG
CCACTTGTCAAAAAAGGCGGTTCGTTCCTAGTAATGAAAGCAGCCCAAGCCGAGCAAGAGCTGCAAACAGCCGAAAAAGC
CATCAAACTATTCGGCGGAAAAGTCGAGGAACACTTCGCTTTCTCCCTACCAGTAGAAGAAAGCGAACGCAATATCTACG
TCATCACGAAAACAAAAGAAACCCCCAACAAATATCCACGCAAACCTGGAACACCCAACAAATTACCAATCGAATAA

Upstream 100 bases:

>100_bases
GAGCTAAAATATATGGTACAAGCCTGTTTAAAAGTATAAACCAAACCCGTCTACCATCCTAAACACGGCAAAAATCGCGA
AAATAAAGTGAGGAAAAAAT

Downstream 100 bases:

>100_bases
ACTAACAAAGTCCTTTCAAACGAAAGGACTTTTTATTTTTAACAGCAACGTCATTTTTTAAAGCTAGTTTCCGCAACTAT
CCACACTAAAATATGAAAAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNPEQFQTALAEKGIELSDTQLKQFHDYFEMLVEWNEKMNLTAITDEKEVYLKHFYDSISAAFYVDFTKFDTICDVGAGA
GFPSLPIKICFPHLKVSIVDSLKKRMTFLDALAEKLGLTDVHFYHDRAETFGQNKAHREKYDLVTARAVARMSVLSELCM
PLVKKGGSFLVMKAAQAEQELQTAEKAIKLFGGKVEEHFAFSLPVEESERNIYVITKTKETPNKYPRKPGTPNKLPIE

Sequences:

>Translated_238_residues
MNPEQFQTALAEKGIELSDTQLKQFHDYFEMLVEWNEKMNLTAITDEKEVYLKHFYDSISAAFYVDFTKFDTICDVGAGA
GFPSLPIKICFPHLKVSIVDSLKKRMTFLDALAEKLGLTDVHFYHDRAETFGQNKAHREKYDLVTARAVARMSVLSELCM
PLVKKGGSFLVMKAAQAEQELQTAEKAIKLFGGKVEEHFAFSLPVEESERNIYVITKTKETPNKYPRKPGTPNKLPIE
>Mature_238_residues
MNPEQFQTALAEKGIELSDTQLKQFHDYFEMLVEWNEKMNLTAITDEKEVYLKHFYDSISAAFYVDFTKFDTICDVGAGA
GFPSLPIKICFPHLKVSIVDSLKKRMTFLDALAEKLGLTDVHFYHDRAETFGQNKAHREKYDLVTARAVARMSVLSELCM
PLVKKGGSFLVMKAAQAEQELQTAEKAIKLFGGKVEEHFAFSLPVEESERNIYVITKTKETPNKYPRKPGTPNKLPIE

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=210, Percent_Identity=30.952380952381, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27080; Mature: 27080

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEQFQTALAEKGIELSDTQLKQFHDYFEMLVEWNEKMNLTAITDEKEVYLKHFYDSIS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AAFYVDFTKFDTICDVGAGAGFPSLPIKICFPHLKVSIVDSLKKRMTFLDALAEKLGLTD
HEEEEEHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VHFYHDRAETFGQNKAHREKYDLVTARAVARMSVLSELCMPLVKKGGSFLVMKAAQAEQE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
LQTAEKAIKLFGGKVEEHFAFSLPVEESERNIYVITKTKETPNKYPRKPGTPNKLPIE
HHHHHHHHHHHCCCHHHHEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPEQFQTALAEKGIELSDTQLKQFHDYFEMLVEWNEKMNLTAITDEKEVYLKHFYDSIS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AAFYVDFTKFDTICDVGAGAGFPSLPIKICFPHLKVSIVDSLKKRMTFLDALAEKLGLTD
HEEEEEHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VHFYHDRAETFGQNKAHREKYDLVTARAVARMSVLSELCMPLVKKGGSFLVMKAAQAEQE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
LQTAEKAIKLFGGKVEEHFAFSLPVEESERNIYVITKTKETPNKYPRKPGTPNKLPIE
HHHHHHHHHHHCCCHHHHEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]