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The map label for this gene is engD [H]

Identifier: 226225332

GI number: 226225332

Start: 2836107

End: 2837207

Strand: Reverse

Name: engD [H]

Synonym: Lm4b_02755

Alternate gene names: 226225332

Gene position: 2837207-2836107 (Counterclockwise)

Preceding gene: 226225333

Following gene: 226225331

Centisome position: 97.41

GC content: 41.24

Gene sequence:

>1101_bases
ATGGCACTTACAGCTGGTATTGTCGGGCTTCCTAATGTTGGGAAATCGACACTTTTTAATGCAATCACAAAAGCAGGAGC
AGAGGCTGCGAATTATCCGTTTGCGACGATTGACCCGAACGTAGGAATTGTTGAAGTTCCTGACCACAGATTAAACAAAC
TAACGGAACTTGTAAAACCGAAAAAGACCGTTCCAACAACTTTTGAATTTACAGATATCGCCGGAATTGTAAAAGGTGCT
AGTAAAGGGGAAGGACTTGGAAACAAATTCTTATCCCATATTCGTCAAGTGGATGCAATATGCCATGTAACTCGTTGTTT
TGATGACGAAAATATCACCCACGTAGAAGGCCGAGTAGACCCGCTAGACGATATTTCTACAATCAACTTAGAACTTATCT
TAGCGGACTTAGAAACAGTAGAGAAGCGCATTGGACGCGTTGAGAAGCTATCTAAACAAAAAGATAAAGATGCTGTGGCT
GAATATAACGTTTTAGTTAAACTACGTGAGGCTTTCGAAAATGACAAACCAGCTCGTGCGATTGAATTTAACGAAGAAGA
AGAGAAAATCGTTCGCAACCTTTTCTTACTTACAAGAAAACCAGTTTTATATGTAGCAAATGTAAGTGAAGAAGATGTTT
CTAGTCCGGACGATAACAAATATGTACAGCAAGTACGCGAATTCGCTGCGAGCGAGAACTCTGAAGTTATCGTAGTATGT
GCTCGCGCTGAGGAAGAAATTGCTGAACTTGAAGACGAAGATAAGCTAGAGTTTTTAGAAGCGCTTGGAATTGAAGAATC
TGGTTTAGACCAATTGATTCGTTCTGCGTATACATTGCTTGGCTTAGCGACTTACTTCACAGCAGGCGTTCAAGAAGTTC
GCGCTTGGACCTTCATCAAAGGAATGAAAGCTCCACAATGTGCCGGAATCATTCATACTGATTTCGAACGTGGATTCATT
CGCGCTGAAGTTGTTGCATATGACGCTTTACTTGAATACGGCTCAGAGCAAGCAGCCAAAGAAGCGGGGAAAGTACGCTT
GGAAGGTAAAGAATACGAAATGAAAGATGGAGATGTTGTTCATTTCCGCTTTAACGTTTAA

Upstream 100 bases:

>100_bases
GGCTTTTTGTTTGAAAAACCTTTATAATATACGTTAGTGAAAAAATGTCTATTTGACAGGCATTTTCAAAATGAAGTTAA
TGAAAAGGAGTTTATATATA

Downstream 100 bases:

>100_bases
TATGTTTCACGTGAAACAATCTTTTGACAAGTAAGTATGTCAAAAGATTGTTTTTTAGGTTAGTTCATAAATTCTGCGTT
ACTGATATACTTCAAAAGAG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGIVEVPDHRLNKLTELVKPKKTVPTTFEFTDIAGIVKGA
SKGEGLGNKFLSHIRQVDAICHVTRCFDDENITHVEGRVDPLDDISTINLELILADLETVEKRIGRVEKLSKQKDKDAVA
EYNVLVKLREAFENDKPARAIEFNEEEEKIVRNLFLLTRKPVLYVANVSEEDVSSPDDNKYVQQVREFAASENSEVIVVC
ARAEEEIAELEDEDKLEFLEALGIEESGLDQLIRSAYTLLGLATYFTAGVQEVRAWTFIKGMKAPQCAGIIHTDFERGFI
RAEVVAYDALLEYGSEQAAKEAGKVRLEGKEYEMKDGDVVHFRFNV

Sequences:

>Translated_366_residues
MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGIVEVPDHRLNKLTELVKPKKTVPTTFEFTDIAGIVKGA
SKGEGLGNKFLSHIRQVDAICHVTRCFDDENITHVEGRVDPLDDISTINLELILADLETVEKRIGRVEKLSKQKDKDAVA
EYNVLVKLREAFENDKPARAIEFNEEEEKIVRNLFLLTRKPVLYVANVSEEDVSSPDDNKYVQQVREFAASENSEVIVVC
ARAEEEIAELEDEDKLEFLEALGIEESGLDQLIRSAYTLLGLATYFTAGVQEVRAWTFIKGMKAPQCAGIIHTDFERGFI
RAEVVAYDALLEYGSEQAAKEAGKVRLEGKEYEMKDGDVVHFRFNV
>Mature_365_residues
ALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGIVEVPDHRLNKLTELVKPKKTVPTTFEFTDIAGIVKGAS
KGEGLGNKFLSHIRQVDAICHVTRCFDDENITHVEGRVDPLDDISTINLELILADLETVEKRIGRVEKLSKQKDKDAVAE
YNVLVKLREAFENDKPARAIEFNEEEEKIVRNLFLLTRKPVLYVANVSEEDVSSPDDNKYVQQVREFAASENSEVIVVCA
RAEEEIAELEDEDKLEFLEALGIEESGLDQLIRSAYTLLGLATYFTAGVQEVRAWTFIKGMKAPQCAGIIHTDFERGFIR
AEVVAYDALLEYGSEQAAKEAGKVRLEGKEYEMKDGDVVHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=44.3243243243243, Blast_Score=311, Evalue=9e-85,
Organism=Homo sapiens, GI58761502, Length=228, Percent_Identity=38.1578947368421, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24308117, Length=112, Percent_Identity=36.6071428571429, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787454, Length=366, Percent_Identity=58.4699453551913, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789574, Length=134, Percent_Identity=29.1044776119403, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=42.6229508196721, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI17508313, Length=100, Percent_Identity=34, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=42.4324324324324, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321773, Length=384, Percent_Identity=38.5416666666667, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321649, Length=158, Percent_Identity=39.2405063291139, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321962, Length=163, Percent_Identity=28.2208588957055, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=40.0611620795107, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24585318, Length=186, Percent_Identity=27.9569892473118, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129375, Length=111, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40684; Mature: 40552

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGIVEVPDHRLNKLTELVKP
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCEEECCCCCEEECCHHHHHHHHHHHCC
KKTVPTTFEFTDIAGIVKGASKGEGLGNKFLSHIRQVDAICHVTRCFDDENITHVEGRVD
CCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
PLDDISTINLELILADLETVEKRIGRVEKLSKQKDKDAVAEYNVLVKLREAFENDKPARA
CCCCCCCEEHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEE
IEFNEEEEKIVRNLFLLTRKPVLYVANVSEEDVSSPDDNKYVQQVREFAASENSEVIVVC
ECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
ARAEEEIAELEDEDKLEFLEALGIEESGLDQLIRSAYTLLGLATYFTAGVQEVRAWTFIK
ECCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMKAPQCAGIIHTDFERGFIRAEVVAYDALLEYGSEQAAKEAGKVRLEGKEYEMKDGDVV
CCCCCCCCCCEECCHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCEECCCCCEE
HFRFNV
EEEECC
>Mature Secondary Structure 
ALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGIVEVPDHRLNKLTELVKP
CCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCEEECCCCCEEECCHHHHHHHHHHHCC
KKTVPTTFEFTDIAGIVKGASKGEGLGNKFLSHIRQVDAICHVTRCFDDENITHVEGRVD
CCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
PLDDISTINLELILADLETVEKRIGRVEKLSKQKDKDAVAEYNVLVKLREAFENDKPARA
CCCCCCCEEHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEE
IEFNEEEEKIVRNLFLLTRKPVLYVANVSEEDVSSPDDNKYVQQVREFAASENSEVIVVC
ECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
ARAEEEIAELEDEDKLEFLEALGIEESGLDQLIRSAYTLLGLATYFTAGVQEVRAWTFIK
ECCHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMKAPQCAGIIHTDFERGFIRAEVVAYDALLEYGSEQAAKEAGKVRLEGKEYEMKDGDVV
CCCCCCCCCCEECCHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCEECCCCCEE
HFRFNV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]