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The map label for this gene is mdtG [H]

Identifier: 226225292

GI number: 226225292

Start: 2786355

End: 2787563

Strand: Reverse

Name: mdtG [H]

Synonym: Lm4b_02715

Alternate gene names: 226225292

Gene position: 2787563-2786355 (Counterclockwise)

Preceding gene: 226225293

Following gene: 226225291

Centisome position: 95.7

GC content: 43.09

Gene sequence:

>1209_bases
ATGGAAAATTGGAAGAAGAATTTGTATGTTGTCTGGGTGGGGTGCTTTCTAACCGGAACCGGATTAAATTTAATCATGCC
ATTTTTACCATTGTATATTGAAGAATTAGGAGTGCATAATCCGGATCAAGTAAGCATGTGGTCAGGGATTGCACTCAGTT
CGACCTTTTTAGTATCAGCTATTATGTCACCAATTTGGGGTAAACTAGCTGACCAAAAAGGGCGGAGAATTATGCTTTTG
CGAGCTGCGCTAGGTATGGCGATAGCGATGATTCTGATGGGACTCGTAAGCAATGTATACCAATTTGTTGGTTTGCGCTT
ATTAATGGGGATTTTTTCAGGATATATTTCAACAGCAAATGCCCTTATTGCTACACAAGTTCCTCGTCATCGTAGCGGCT
GGGCTCTCGGGGCATTATCCACGGCGGCGGTTTCGGGTGTGCTAATTGGCCCGTTAATTGGCGGAGCGCTGTCTGATACA
TTTGGCGTCAGACCAGTGTTTTACATCACAGGAGTACTTCTTTTAGGTAGCTTTTTCCTCACACTGTTCTTTGTGAAAGA
GAAATTTACACCAGTAGAAAAAAAGGAAATGCGGTCGGGGAAAGAAGTTTTCTTGTCGCTTAAAAACCCGGGCTTGATTA
TTTCCTTATTTATTACGACAATGATGATTCAAATTGCGTCCAACTCAGTGAACCCGATTTTGACATTATACGTGCGTGAT
TTGGCAGGAAATGCGCAAAACATCGCTTTTATTAGTGGGATGATTGCATCTGTTCCAGGGGTTGCGGCACTTATTGCGGC
ACCGAGACTTGGGAGATGGGGAGACCGAATTGGTTCGGAGCGAATATTGTTAGGTGCGCTCATAGGTTCAATGTTACTCC
AAATTCCAATGGCTTTCGCACAAAACCCACTACAACTTGGGATTTTACGTTTCTTATTAGGGCTTACAGACGGCGCCTTG
CTTCCAGCGGTTCAATCATTGCTTACAAAAAATACACCGCGCGAGGTTTCAGGTCGGATTTTTGGTTATAATCAATCGTT
TCAATACATTGGGAATGTTATCGGACCGCTTGTTGGGTCAAGTGTTGCCGCCCATTTTGGTTACGGAGATGTCTTCCTCG
TAGTTGCAGGCTTTATTTTTATCAATGTACTAATCAGTTTTTATTTCAACCGAAAAATGCATGGAGAGAAGGGAAATCAT
GCCAACTGA

Upstream 100 bases:

>100_bases
CATTTTACGCAAATATAGCAAAAAAGCGAAGGGTTTAGTTTTACTTTCGCTTTTTTTGTGGAATAATTAAGGATAACGAT
AGAAGAATCTAGGTGGGTTT

Downstream 100 bases:

>100_bases
ACTAATAAAATTACCAGGAATAGGTAAAAAAATGGTGTTAATGTTAAATGAAATTGGGGTTGAAGAGGTTGCTGATTTAA
AAGGGAAGAATCCGCTTGAG

Product: drug-efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MENWKKNLYVVWVGCFLTGTGLNLIMPFLPLYIEELGVHNPDQVSMWSGIALSSTFLVSAIMSPIWGKLADQKGRRIMLL
RAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTANALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDT
FGVRPVFYITGVLLLGSFFLTLFFVKEKFTPVEKKEMRSGKEVFLSLKNPGLIISLFITTMMIQIASNSVNPILTLYVRD
LAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMAFAQNPLQLGILRFLLGLTDGAL
LPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIGPLVGSSVAAHFGYGDVFLVVAGFIFINVLISFYFNRKMHGEKGNH
AN

Sequences:

>Translated_402_residues
MENWKKNLYVVWVGCFLTGTGLNLIMPFLPLYIEELGVHNPDQVSMWSGIALSSTFLVSAIMSPIWGKLADQKGRRIMLL
RAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTANALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDT
FGVRPVFYITGVLLLGSFFLTLFFVKEKFTPVEKKEMRSGKEVFLSLKNPGLIISLFITTMMIQIASNSVNPILTLYVRD
LAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMAFAQNPLQLGILRFLLGLTDGAL
LPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIGPLVGSSVAAHFGYGDVFLVVAGFIFINVLISFYFNRKMHGEKGNH
AN
>Mature_402_residues
MENWKKNLYVVWVGCFLTGTGLNLIMPFLPLYIEELGVHNPDQVSMWSGIALSSTFLVSAIMSPIWGKLADQKGRRIMLL
RAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTANALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDT
FGVRPVFYITGVLLLGSFFLTLFFVKEKFTPVEKKEMRSGKEVFLSLKNPGLIISLFITTMMIQIASNSVNPILTLYVRD
LAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMAFAQNPLQLGILRFLLGLTDGAL
LPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIGPLVGSSVAAHFGYGDVFLVVAGFIFINVLISFYFNRKMHGEKGNH
AN

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=390, Percent_Identity=62.0512820512821, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.0837696335079, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319871, Length=137, Percent_Identity=29.9270072992701, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43479; Mature: 43479

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENWKKNLYVVWVGCFLTGTGLNLIMPFLPLYIEELGVHNPDQVSMWSGIALSSTFLVSA
CCCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHH
IMSPIWGKLADQKGRRIMLLRAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTAN
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDTFGVRPVFYITGVLLLGSFFL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLFFVKEKFTPVEKKEMRSGKEVFLSLKNPGLIISLFITTMMIQIASNSVNPILTLYVRD
HHHHHHHHCCCCCHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMAFA
HCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHC
QNPLQLGILRFLLGLTDGALLPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIGPLVGS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHH
SVAAHFGYGDVFLVVAGFIFINVLISFYFNRKMHGEKGNHAN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MENWKKNLYVVWVGCFLTGTGLNLIMPFLPLYIEELGVHNPDQVSMWSGIALSSTFLVSA
CCCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHH
IMSPIWGKLADQKGRRIMLLRAALGMAIAMILMGLVSNVYQFVGLRLLMGIFSGYISTAN
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIATQVPRHRSGWALGALSTAAVSGVLIGPLIGGALSDTFGVRPVFYITGVLLLGSFFL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TLFFVKEKFTPVEKKEMRSGKEVFLSLKNPGLIISLFITTMMIQIASNSVNPILTLYVRD
HHHHHHHHCCCCCHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAGNAQNIAFISGMIASVPGVAALIAAPRLGRWGDRIGSERILLGALIGSMLLQIPMAFA
HCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHC
QNPLQLGILRFLLGLTDGALLPAVQSLLTKNTPREVSGRIFGYNQSFQYIGNVIGPLVGS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCEEECCCCHHHHHHHHHHHHHHH
SVAAHFGYGDVFLVVAGFIFINVLISFYFNRKMHGEKGNHAN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA